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Literature summary extracted from

  • Sytykiewicz, H.; Sprawka, I.; Czerniewicz, P.; Sempruch, C.; Leszczy?ski, B.; Sikora, M.
    Biochemical characterisation of chlorophyllase from leaves of selected Prunus species--a comparative study (2013), Acta Biochim. Pol., 60, 457-465.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.14 2-mercaptoethanol subtle stimulation of enzymatic activity at 10 mM Prunus domestica
3.1.1.14 2-mercaptoethanol subtle stimulation of enzymatic activity at 10 mM Prunus padus
3.1.1.14 2-mercaptoethanol subtle stimulation of enzymatic activity at 10 mM Prunus cerasus
3.1.1.14 dithiothreitol subtle stimulation of enzymatic activity at 10 mM Prunus domestica
3.1.1.14 dithiothreitol subtle stimulation of enzymatic activity at 10 mM Prunus padus
3.1.1.14 dithiothreitol subtle stimulation of enzymatic activity at 10 mM Prunus cerasus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.14 Ag+ 82% residual activity at 10 mM Prunus cerasus
3.1.1.14 Ag+ 89% residual activity at 10 mM Prunus domestica
3.1.1.14 Ag+ 85% residual activity at 10 mM Prunus padus
3.1.1.14 Al3+ 52% residual activity at 10 mM Prunus cerasus
3.1.1.14 Al3+ 56% residual activity at 10 mM Prunus domestica
3.1.1.14 Al3+ 49% residual activity at 10 mM Prunus padus
3.1.1.14 Bi3+ 52% residual activity at 10 mM Prunus cerasus
3.1.1.14 Bi3+ 58% residual activity at 10 mM Prunus domestica
3.1.1.14 Bi3+ 55% residual activity at 10 mM Prunus padus
3.1.1.14 Co2+ 75% residual activity at 10 mM Prunus cerasus
3.1.1.14 Co2+ 78% residual activity at 10 mM Prunus domestica
3.1.1.14 Co2+ 72% residual activity at 10 mM Prunus padus
3.1.1.14 Cu2+ 39% residual activity at 10 mM Prunus cerasus
3.1.1.14 Cu2+ 42% residual activity at 10 mM Prunus domestica
3.1.1.14 Cu2+ 34% residual activity at 10 mM Prunus padus
3.1.1.14 diethyldicarbonate 83% residual activity at 10 mM Prunus cerasus
3.1.1.14 diethyldicarbonate 70% residual activity at 10 mM Prunus domestica
3.1.1.14 diethyldicarbonate 72% residual activity at 10 mM Prunus padus
3.1.1.14 Fe2+ 31% residual activity at 10 mM Prunus cerasus
3.1.1.14 Fe2+ 29% residual activity at 10 mM Prunus domestica
3.1.1.14 Fe2+ 35% residual activity at 10 mM Prunus padus
3.1.1.14 Fe3+ 73% residual activity at 10 mM Prunus cerasus
3.1.1.14 Fe3+ 71% residual activity at 10 mM Prunus domestica
3.1.1.14 Fe3+ 78% residual activity at 10 mM Prunus padus
3.1.1.14 Hg2+ 22% residual activity at 10 mM Prunus cerasus
3.1.1.14 Hg2+ 25% residual activity at 10 mM Prunus domestica
3.1.1.14 Hg2+ 20% residual activity at 10 mM Prunus padus
3.1.1.14 Mn2+ 72% residual activity at 10 mM Prunus cerasus
3.1.1.14 Mn2+ 78% residual activity at 10 mM Prunus domestica
3.1.1.14 Mn2+ 73% residual activity at 10 mM Prunus padus
3.1.1.14 phenylmethylsulfonyl fluoride 69% residual activity at 10 mM Prunus cerasus
3.1.1.14 phenylmethylsulfonyl fluoride 58% residual activity at 10 mM Prunus domestica
3.1.1.14 phenylmethylsulfonyl fluoride 65% residual activity at 10 mM Prunus padus
3.1.1.14 Zn2+ 51% residual activity at 10 mM Prunus cerasus
3.1.1.14 Zn2+ 59% residual activity at 10 mM Prunus domestica
3.1.1.14 Zn2+ 54% residual activity at 10 mM Prunus padus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.14 0.0028
-
chlorophyll a isoform Chlase F1, at pH 7.0 and 30°C Prunus padus
3.1.1.14 0.003
-
chlorophyll b isoform Chlase F1, at pH 7.0 and 30°C Prunus padus
3.1.1.14 0.0031
-
chlorophyll a isoform Chlase F2, at pH 7.0 and 30°C Prunus padus
3.1.1.14 0.0034
-
chlorophyll a isoform Chlase F1, at pH 7.0 and 30°C Prunus cerasus
3.1.1.14 0.0035
-
chlorophyll a isoform Chlase F1, at pH 7.0 and 30°C Prunus domestica
3.1.1.14 0.0035
-
chlorophyll b isoform Chlase F2, at pH 7.0 and 30°C Prunus padus
3.1.1.14 0.0037
-
chlorophyll b isoform Chlase F1, at pH 7.0 and 30°C Prunus cerasus
3.1.1.14 0.0038
-
chlorophyll b isoform Chlase F2, at pH 7.0 and 30°C Prunus cerasus
3.1.1.14 0.0039
-
chlorophyll b isoform Chlase F1, at pH 7.0 and 30°C Prunus domestica
3.1.1.14 0.0042
-
chlorophyll a isoform Chlase F2, at pH 7.0 and 30°C Prunus cerasus
3.1.1.14 0.0048
-
chlorophyll a isoform Chlase F2, at pH 7.0 and 30°C Prunus domestica
3.1.1.14 0.0061
-
chlorophyll b isoform Chlase F2, at pH 7.0 and 30°C Prunus domestica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.14 Mg2+ subtle stimulation of enzymatic activity at 10 mM Prunus domestica
3.1.1.14 Mg2+ subtle stimulation of enzymatic activity at 10 mM Prunus padus
3.1.1.14 Mg2+ subtle stimulation of enzymatic activity at 10 mM Prunus cerasus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.14 35000
-
x * 35000, isoform Chlase F2, SDS-PAGE Prunus domestica
3.1.1.14 40000
-
x * 40000, isoform Chlase F2, SDS-PAGE Prunus padus
3.1.1.14 60000
-
x * 60000, isoform Chlase F2, SDS-PAGE Prunus cerasus
3.1.1.14 90000
-
x * 90000, isoform Chlase F1, SDS-PAGE Prunus padus
3.1.1.14 100000
-
x * 100000, isoform Chlase F1, SDS-PAGE Prunus domestica
3.1.1.14 100000
-
x * 100000, isoform Chlase F1, SDS-PAGE Prunus cerasus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.14 chlorophyll a + H2O Prunus domestica
-
phytol + chlorophyllide a
-
?
3.1.1.14 chlorophyll a + H2O Prunus padus
-
phytol + chlorophyllide a
-
?
3.1.1.14 chlorophyll a + H2O Prunus cerasus
-
phytol + chlorophyllide a
-
?
3.1.1.14 chlorophyll b + H2O Prunus domestica
-
phytol + chlorophyllide b
-
?
3.1.1.14 chlorophyll b + H2O Prunus padus
-
phytol + chlorophyllide b
-
?
3.1.1.14 chlorophyll b + H2O Prunus cerasus
-
phytol + chlorophyllide b
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.14 Prunus cerasus
-
sour cherry
-
3.1.1.14 Prunus domestica
-
European plum
-
3.1.1.14 Prunus padus
-
bird cherry
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.14 ammonium sulfate precipitation and Sephadex G-200gel filtration Prunus domestica
3.1.1.14 ammonium sulfate precipitation and Sephadex G-200gel filtration Prunus padus
3.1.1.14 ammonium sulfate precipitation and Sephadex G-200gel filtration Prunus cerasus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.14 leaf
-
Prunus domestica
-
3.1.1.14 leaf
-
Prunus padus
-
3.1.1.14 leaf
-
Prunus cerasus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.14 0.15
-
unpurified homogenate, at pH 7.0 and 30°C Prunus padus
3.1.1.14 0.51
-
unpurified homogenate, at pH 7.0 and 30°C Prunus cerasus
3.1.1.14 0.68
-
unpurified homogenate, at pH 7.0 and 30°C Prunus domestica
3.1.1.14 5.53
-
after 3.73fold purification, isoform Chlase F1, at pH 7.0 and 30°C Prunus padus
3.1.1.14 7.42
-
after 3.73fold purification, isoform Chlase F2, at pH 7.0 and 30°C Prunus padus
3.1.1.14 9.52
-
after 18.67fold purification, isoform Chlase F2, at pH 7.0 and 30°C Prunus cerasus
3.1.1.14 14.75
-
after 28.92fold purification, isoform Chlase F1, at pH 7.0 and 30°C Prunus cerasus
3.1.1.14 16.92
-
after 24.88fold purification, isoform Chlase F2, at pH 7.0 and 30°C Prunus domestica
3.1.1.14 19.47
-
after 28.63fold purification, isoform Chlase F1, at pH 7.0 and 30°C Prunus domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.14 chlorophyll a + H2O
-
Prunus domestica phytol + chlorophyllide a
-
?
3.1.1.14 chlorophyll a + H2O
-
Prunus padus phytol + chlorophyllide a
-
?
3.1.1.14 chlorophyll a + H2O
-
Prunus cerasus phytol + chlorophyllide a
-
?
3.1.1.14 chlorophyll b + H2O
-
Prunus domestica phytol + chlorophyllide b
-
?
3.1.1.14 chlorophyll b + H2O
-
Prunus padus phytol + chlorophyllide b
-
?
3.1.1.14 chlorophyll b + H2O
-
Prunus cerasus phytol + chlorophyllide b
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.14 ? x * 100000, isoform Chlase F1, SDS-PAGE Prunus domestica
3.1.1.14 ? x * 100000, isoform Chlase F1, SDS-PAGE Prunus cerasus
3.1.1.14 ? x * 35000, isoform Chlase F2, SDS-PAGE Prunus domestica
3.1.1.14 ? x * 40000, isoform Chlase F2, SDS-PAGE Prunus padus
3.1.1.14 ? x * 60000, isoform Chlase F2, SDS-PAGE Prunus cerasus
3.1.1.14 ? x * 90000, isoform Chlase F1, SDS-PAGE Prunus padus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.14 CHlase F1 isoform Prunus domestica
3.1.1.14 CHlase F1 isoform Prunus padus
3.1.1.14 CHlase F1 isoform Prunus cerasus
3.1.1.14 Chlase F2 isoform Prunus domestica
3.1.1.14 Chlase F2 isoform Prunus padus
3.1.1.14 Chlase F2 isoform Prunus cerasus
3.1.1.14 chlorophyll chlorophyllido-hydrolase
-
Prunus domestica
3.1.1.14 chlorophyll chlorophyllido-hydrolase
-
Prunus padus
3.1.1.14 chlorophyll chlorophyllido-hydrolase
-
Prunus cerasus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.14 7.6
-
-
Prunus domestica
3.1.1.14 7.8
-
-
Prunus padus
3.1.1.14 8
-
-
Prunus cerasus