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Literature summary extracted from

  • Kim, T.; Spiegel, D.
    Serendipitous discovery of two highly selective inhibitors of bacterial luciferase (2013), Tetrahedron, 69, 7692-7698.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.3 expressed in Escherichia coli BL21(DE3) cells Vibrio harveyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.3 1-Dodecanol
-
 Vibrio harveyi
1.14.14.3 1-Tetradecanol
-
 Vibrio harveyi
1.14.14.3 2,2-diphenylpropylamine
-
 Vibrio harveyi
1.14.14.3 8-Anilino-1-naphthalenesulfonate
-
 Vibrio harveyi
1.14.14.3 dodecanenitrile
-
 Vibrio harveyi
1.14.14.3 dodecylamine
-
 Vibrio harveyi
1.14.14.3 N,N-Dimethylaniline
-
 Vibrio harveyi
1.14.14.3 N-phenacylthiazolium bromide highly selective inhibition Vibrio harveyi
1.14.14.3 Pargyline
-
 Vibrio harveyi
1.14.14.3 pifithrin-alpha highly selective inhibitor in vivo and in vitro Vibrio harveyi
1.14.14.3 SKF-525A
-
 Vibrio harveyi
1.14.14.3 trans-2-decenal
-
 Vibrio harveyi
1.14.14.3 undecane
-
 Vibrio harveyi

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.3 Vibrio harveyi
-
-
-
1.14.14.3 Vibrio harveyi BB120
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.3 RCHO + FMNH2 + O2
-
 Vibrio harveyi RCOOH + FMN + H2O + hv
-
 ?
1.14.14.3 RCHO + FMNH2 + O2
-
 Vibrio harveyi BB120 RCOOH + FMN + H2O + hv
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.3 luciferase
-
 Vibrio harveyi
1.14.14.3 Lux
-
 Vibrio harveyi

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.14.3 0.00003
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi dodecylamine
1.14.14.3 0.00008
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi dodecanenitrile
1.14.14.3 0.000122
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi undecane
1.14.14.3 0.000356
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi 1-Tetradecanol
1.14.14.3 0.000482
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi 1-Dodecanol
1.14.14.3 0.005
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi trans-2-decenal
1.14.14.3 0.017
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi pifithrin-alpha
1.14.14.3 0.03
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi 8-Anilino-1-naphthalenesulfonate
1.14.14.3 0.09
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi SKF-525A
1.14.14.3 0.095
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi N-phenacylthiazolium bromide
1.14.14.3 0.1
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi N,N-Dimethylaniline
1.14.14.3 0.115
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi 2,2-diphenylpropylamine
1.14.14.3 1
-
 in 50 mM phosphate buffer, pH 7.0 at 23°C Vibrio harveyi Pargyline