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Literature summary extracted from

  • Orzolek, A.; Wysocki, P.; Strzezek, J.; Kordan, W.
    Superoxide dismutase (SOD) in boar spermatozoa: purification, biochemical properties and changes in activity during semen storage (16?C) in different extenders (2013), Reprod. Biol., 13, 34-40.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 2-mercaptoethanol 15% inhibition Sus scrofa
1.15.1.1 diethyldithiocarbamate 40% inhibition Sus scrofa
1.15.1.1 H2O2 65% inhibition Sus scrofa
1.15.1.1 additional information urea and iodoacetamide do not affect the enzymatic activity Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 30000
-
 2 * 30000, SDS-PAGE Sus scrofa
1.15.1.1 69000
-
-
 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Sus scrofa
-
 Large White boars
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 native enzyme from sperm 7.5-38fold, superoxide dismutase release from spermatozoa after cold shock and homogenization, followed by ion exchange chromatography and gel filtration Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 sperm changes in SOD activity in boar sperm during preservation at 16°C, overview Sus scrofa
-
1.15.1.1 spermatozoon
-
 Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
1.15.1.1 16°C, changes in superoxide dismutase activity in boar sperm during preservation, overview Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 additional information superoxide dismutase activity is measured by the inhibition of nitro blue tetrazolium reduction in the presence of the superoxide anion generated by the xanthine and xanthine oxidase system Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 homodimer 2 * 30000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 SOD
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 20
-
 monomeric enzyme Sus scrofa
1.15.1.1 20 45 dimeric enzyme, broad optimum Sus scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.15.1.1 20 45 high activity within this range Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 10
-
-
 Sus scrofa