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Literature summary extracted from
- Substrate-dependent dynamics of UDP-galactopyranose mutase: implications for drug design (2013), Protein Sci., 22, 1490-1501.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.4.99.9 | drug development | since the enzyme does not exist in Homo sapiens, the parasite enzyme is a target for drug design | Trypanosoma cruzi |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.4.99.9 | crystal structure analysis, molecular dynamics simulations and modeling, overview | Trypanosoma cruzi |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.9 | R176A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Trypanosoma cruzi |
| 5.4.99.9 | R327A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Trypanosoma cruzi |
| 5.4.99.9 | Y317F | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Trypanosoma cruzi |
| 5.4.99.9 | Y395F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Trypanosoma cruzi |
| 5.4.99.9 | Y429F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Trypanosoma cruzi |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.9 | 0.0334 | - |
UDP-alpha-D-galactopyranose | mutant Y317F, pH 7.5, 37°C | Trypanosoma cruzi |  |
| 5.4.99.9 | 0.09 | - |
UDP-alpha-D-galactopyranose | mutant R327A, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 0.091 | - |
UDP-alpha-D-galactopyranose | mutant Y395F, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 0.1 | - |
UDP-alpha-D-galactopyranose | mutant Y429F, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 0.134 | - |
UDP-alpha-D-galactopyranose | mutant R176A, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 0.14 | - |
UDP-alpha-D-galactopyranose | wild-type enzyme, pH 7.5, 37°C | Trypanosoma cruzi | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.9 | UDP-alpha-D-galactopyranose | Trypanosoma cruzi | - |
UDP-alpha-D-galactofuranose | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.9 | no activity in Homo sapiens | - |
- |
- |
| 5.4.99.9 | Trypanosoma cruzi | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.9 | UDP-alpha-D-galactopyranose | - |
Trypanosoma cruzi | UDP-alpha-D-galactofuranose | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.9 | UGM | - |
Trypanosoma cruzi |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.9 | 37 | - |
assay at | Trypanosoma cruzi |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.9 | 0.0224 | - |
UDP-alpha-D-galactopyranose | mutant R176A, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 0.248 | - |
UDP-alpha-D-galactopyranose | mutant Y395F, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 0.291 | - |
UDP-alpha-D-galactopyranose | mutant Y317F, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 4.26 | - |
UDP-alpha-D-galactopyranose | mutant R327A, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 6.296 | - |
UDP-alpha-D-galactopyranose | mutant Y429F, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 13.4 | - |
UDP-alpha-D-galactopyranose | wild-type enzyme, pH 7.5, 37°C | Trypanosoma cruzi | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.9 | 7.5 | - |
assay at | Trypanosoma cruzi |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.9 | FAD | - |
Trypanosoma cruzi | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.99.9 | additional information | molecular details of the mechanism that controls the uptake and retention of the substrate in the presence or absence of an active site ligand, overview. Interactions with the substrate diphosphate moiety are especially important for stabilizing the closed active site. Protein dynamics play a key role in substrate recognition by UDP-galactopyranose mutases. Residues Arg176, Asn201, and Tyr317, Tyr34, Tyr429, and Arg327 are involved in the active site | Trypanosoma cruzi |
| 5.4.99.9 | physiological function | the flavoenzyme UDP-galactopyranose mutase catalyzes the conversion of UDP-galactopyranose to UDP-galactofuranose, a precursor of the cell surface beta-galactofuranose that is involved in the virulence of the pathogen | Trypanosoma cruzi |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.9 | 0.00017 | - |
UDP-alpha-D-galactopyranose | mutant R176A, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 0.00087 | - |
UDP-alpha-D-galactopyranose | mutant Y317F, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 0.0027 | - |
UDP-alpha-D-galactopyranose | mutant Y395F, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 0.047 | - |
UDP-alpha-D-galactopyranose | mutant R327A, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 0.063 | - |
UDP-alpha-D-galactopyranose | mutant Y429F, pH 7.5, 37°C | Trypanosoma cruzi | |
| 5.4.99.9 | 0.093 | - |
UDP-alpha-D-galactopyranose | wild-type enzyme, pH 7.5, 37°C | Trypanosoma cruzi | |
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