Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Celeste, L.R.; Chai, G.; Bielak, M.; Minor, W.; Lovelace, L.L.; Lebioda, L.
    Mechanism of N10-formyltetrahydrofolate synthetase derived from complexes with intermediates and inhibitors (2012), Protein Sci., 21, 219-228.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.3 expressed in Escherichia coli strain Y1 Moorella thermoacetica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.3 native protein, folate complex, ADP-formylphosphate complex, and ZD9331-formylphosphate complex, hanging drop vapor diffusion method. High salt conditions contain 38-46% (w/v) saturated ammonium sulfate, 1 mM , and dithiohtreitol, 1-3.5% (w/v) PEG 1000 or PEG 1450, in 50-75 mM KMB (pH 7.0-8.0). Low salt conditions contain 18-22% (w/v) PEG 6-8K, 0.2 M ammonium sulfate, 1 mM dithiothreitol, in 75 mM KMB pH 7.0-8.5 Moorella thermoacetica

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.3 folate
-
Moorella thermoacetica
6.3.4.3 ZD9331
-
Moorella thermoacetica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.3 60000
-
4 * 60000, X-ray crystallography Moorella thermoacetica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate Moorella thermoacetica
-
ADP + phosphate + 10-formyltetrahydrofolate
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.3 Moorella thermoacetica P21164
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.3 heparin agarose column chromatography and phenyl Sepharose column chromatography Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate
-
Moorella thermoacetica ADP + phosphate + 10-formyltetrahydrofolate
-
?

Subunits

EC Number Subunits Comment Organism
6.3.4.3 homotetramer 4 * 60000, X-ray crystallography Moorella thermoacetica

Synonyms

EC Number Synonyms Comment Organism
6.3.4.3 FTHFS
-
Moorella thermoacetica
6.3.4.3 N10-formyltetrahydrofolate synthetase
-
Moorella thermoacetica

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.3 ATP
-
Moorella thermoacetica