Literature summary extracted from
Chhabra, G.; Mathur, D.; Dixit, A.; Garg, L.C.
Heterologous expression and biochemical characterization of recombinant alpha phosphoglucomutase from Mycobacterium tuberculosis H37Rv (2012), Protein Expr. Purif., 85, 117-124.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.4.2.2 |
gene pgmA, sequence comparison, phylogenetic analysis and expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies |
Mycobacterium tuberculosis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.4.2.2 |
D-fructose 2,6-diphosphate |
noncompetitive inhibitor |
Mycobacterium tuberculosis |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
5.4.2.2 |
additional information |
- |
additional information |
Michaelis-Menten kinetics |
Mycobacterium tuberculosis |
|
5.4.2.2 |
0.1 |
2 |
alpha-D-glucose 1-phosphate |
recombinant enzyme, pH 7.4, 30°C |
Mycobacterium tuberculosis |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
5.4.2.2 |
Mg2+ |
required |
Mycobacterium tuberculosis |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
5.4.2.2 |
alpha-D-glucose 1-phosphate |
Mycobacterium tuberculosis |
- |
D-glucose 6-phosphate |
- |
r |
|
5.4.2.2 |
alpha-D-glucose 1-phosphate |
Mycobacterium tuberculosis H37Rv |
- |
D-glucose 6-phosphate |
- |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.4.2.2 |
Mycobacterium tuberculosis |
I6Y2G3 |
gene pgmA |
- |
5.4.2.2 |
Mycobacterium tuberculosis H37Rv |
I6Y2G3 |
gene pgmA |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.4.2.2 |
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, protein refolding, and ultrafiltration |
Mycobacterium tuberculosis |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
5.4.2.2 |
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) inclusion bodies |
Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
5.4.2.2 |
67.5 |
- |
purified recombinant enzyme, pH 7.4, 30°C |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.4.2.2 |
alpha-D-glucose 1-phosphate |
- |
Mycobacterium tuberculosis |
D-glucose 6-phosphate |
- |
r |
|
5.4.2.2 |
alpha-D-glucose 1-phosphate |
- |
Mycobacterium tuberculosis H37Rv |
D-glucose 6-phosphate |
- |
r |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
5.4.2.2 |
More |
four-domain architecture, homology modeling, overview |
Mycobacterium tuberculosis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.4.2.2 |
alpha phosphoglucomutase |
- |
Mycobacterium tuberculosis |
5.4.2.2 |
PGM |
- |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
5.4.2.2 |
30 |
40 |
recombinant enzyme |
Mycobacterium tuberculosis |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
5.4.2.2 |
70.31 |
- |
alpha-D-glucose 1-phosphate |
recombinant enzyme, pH 7.4, 30°C |
Mycobacterium tuberculosis |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
5.4.2.2 |
7.4 |
- |
assay at |
Mycobacterium tuberculosis |
pH Stability
EC Number |
pH Stability |
pH Stability Maximum |
Comment |
Organism |
---|
5.4.2.2 |
6.5 |
7.4 |
purified recombinant enzyme, stable at |
Mycobacterium tuberculosis |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
5.4.2.2 |
additional information |
structure homology modeling, overview |
Mycobacterium tuberculosis |
5.4.2.2 |
physiological function |
the enzyme plays an important role in polysaccharide capsule formation and virulence in a number of bacterial pathogens |
Mycobacterium tuberculosis |
kcat/KM [mM/s]
EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Comment |
Organism |
Structure |
---|
5.4.2.2 |
58.4 |
- |
alpha-D-glucose 1-phosphate |
recombinant enzyme, pH 7.4, 30°C |
Mycobacterium tuberculosis |
|