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Literature summary extracted from
- Purification and characterization of the recombinant human prostaglandin H synthase-2 expressed in Pichia pastoris (2012), Protein Expr. Purif., 83, 182-189.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.99.1 | functional expression of C-terminally or N-terminally His6-tagged and non-tagged PGHS-2 in Pichia pastoris strain GS115, GS117 and KM71 using native or yeast signal sequences, method evaluation by enzyme activity, overview | Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.99.1 | membrane | membrane associated glycoprotein | Homo sapiens | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.1 | arachidonate + AH2 + 2 O2 | Homo sapiens | - |
prostaglandin H2 + A + H2O | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.1 | Homo sapiens | P23219 | - |
- |
| 1.14.99.1 | Homo sapiens | P35354 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.14.99.1 | glycoprotein | membrane associated glycoprotein | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.99.1 | recombinant C-terminally or N-terminally His6-tagged PGHS-2 from Pichia pastoris strain GS115 microsomes by ultracentrifugation, nickel affinity and anion exchange chromatography, purification of the C-terminally His6 tagged hPGHS-2 is more efficient, relative efficiency of the detergents used for solubilization of the recombinant hPGHS-2, CHAPS is most effective, method evaluation, overview | Homo sapiens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 4220 | - |
purified recombinant His6-tagged PGHS-2, pH 8.0, 37°C | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.1 | arachidonate + AH2 + 2 O2 | - |
Homo sapiens | prostaglandin H2 + A + H2O | - |
? | |
| 1.14.99.1 | arachidonate + reduced N,N,N',N'-tetramethylphenylenediamine + 2 O2 | - |
Homo sapiens | prostaglandin H2 + oxidized N,N,N',N'-tetramethylphenylenediamine + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.99.1 | PGHS-1 | - |
Homo sapiens |
| 1.14.99.1 | PGHS-2 | - |
Homo sapiens |
| 1.14.99.1 | prostaglandin H synthase-1 | - |
Homo sapiens |
| 1.14.99.1 | prostaglandin H synthase-2 | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.1 | 8 | - |
assay at | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.1 | additional information | peroxidase activity of PGHS requires reducing co-substrates as electron donors | Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.99.1 | metabolism | prostaglandin H synthase-1 catalyzes the first two steps in prostaglandin synthesis | Homo sapiens |
| 1.14.99.1 | metabolism | prostaglandin H synthase-2 catalyzes the first two steps in prostaglandin synthesis | Homo sapiens |
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Release 2023.1 (January 2023)
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