EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.6.1 | Azotobacter vinelandii | - |
- |
- |
1.18.6.1 | Azotobacter vinelandii DJ1260 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.6.1 | diazene + ? | modified nitrogenase variant V70A/H195Q under turnover conditions using diazene, methyldiazene, or hydrazine as substrate traps a common S = 1/2 intermediate. Such samples also contain a common intermediate with FeMo-co in an integer-spin state having a ground-state non-Kramers doublet. This species, designated H, has NH2 bound to FeMo-co and corresponds to the penultimate intermediate of N2 hydrogenation, the state formed after the accumulation of seven electrons/protons and the release of the first NH3, the S = 1/2 species corresponds to the final intermediate in N2 reduction, the state formed after accumulation of eight electrons/protons, with NH3 still bound to FeMo-co prior to release and regeneration of resting-state FeMo-co | Azotobacter vinelandii | ? | - |
? | |
1.18.6.1 | diazene + ? | modified nitrogenase variant V70A/H195Q under turnover conditions using diazene, methyldiazene, or hydrazine as substrate traps a common S = 1/2 intermediate. Such samples also contain a common intermediate with FeMo-co in an integer-spin state having a ground-state non-Kramers doublet. This species, designated H, has NH2 bound to FeMo-co and corresponds to the penultimate intermediate of N2 hydrogenation, the state formed after the accumulation of seven electrons/protons and the release of the first NH3, the S = 1/2 species corresponds to the final intermediate in N2 reduction, the state formed after accumulation of eight electrons/protons, with NH3 still bound to FeMo-co prior to release and regeneration of resting-state FeMo-co | Azotobacter vinelandii DJ1260 | ? | - |
? | |
1.18.6.1 | hydrazine + ? | - |
Azotobacter vinelandii | ? | - |
? | |
1.18.6.1 | hydrazine + ? | - |
Azotobacter vinelandii DJ1260 | ? | - |
? | |
1.18.6.1 | methyldiazene + ? | - |
Azotobacter vinelandii | ? | - |
? | |
1.18.6.1 | methyldiazene + ? | - |
Azotobacter vinelandii DJ1260 | ? | - |
? | |
1.18.6.1 | additional information | draft mechanism for N2 reduction by nitrogenase. Diazene binds to the one-electron reduced intermediate with the release of H2, and enters the N2 pathway as the final form of the four-electron reduced state. In contrast, N2H4 instead binds to one-electron reduced intermediate, as is proposed for another two-electron substrate, C2H2, and joins the N2 pathway at a stage corresponding to the seven-electron reduced intermediate in the N2 reduction scheme | Azotobacter vinelandii | ? | - |
? | |
1.18.6.1 | additional information | draft mechanism for N2 reduction by nitrogenase. Diazene binds to the one-electron reduced intermediate with the release of H2, and enters the N2 pathway as the final form of the four-electron reduced state. In contrast, N2H4 instead binds to one-electron reduced intermediate, as is proposed for another two-electron substrate, C2H2, and joins the N2 pathway at a stage corresponding to the seven-electron reduced intermediate in the N2 reduction scheme | Azotobacter vinelandii DJ1260 | ? | - |
? |