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Literature summary extracted from

  • Chen, C.; Cheng, G.; Hao, H.; Dai, M.; Wang, X.; Huang, L.; Liu, Z.; Yuan, Z.
    Mechanism of porcine liver xanthine oxidoreductase mediated N-oxide reduction of cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide as revealed by docking and mutagenesis studies (2013), PLoS ONE, 8, e73912.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.4 XOR is cloned and heterologously expressed in Spodoptera frugiperda insect cells Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.4 D430H Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) slightly increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) slightly decreased compared to wild-type, Vmax (xanthine) slightly decreased to wild-type Sus scrofa
1.17.1.4 D431A Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) slightly increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) increased compared to wild-type, Km (xanthine) slightly increased compared to wild-type, Vmax (xanthine) increased to wild-type Sus scrofa
1.17.1.4 G47A Km and Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) increased compared to wild-type, Km and Vmax (xanthine) slightly increased compared to wild-type Sus scrofa
1.17.1.4 K1230A Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) 2.5fold increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) 2fold increased compared to wild-type, Vmax (xanthine) 2fold decreased to wild-type Sus scrofa
1.17.1.4 N352A Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) slightly increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) slightly decreased compared to wild-type, Km (xanthine) slightly increased compared to wild-type, Vmax (xanthine) slightly decreased compared to wild-type Sus scrofa
1.17.1.4 R427E Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) slightly increased compared to wild-type, Vmax (xanthine) comparable to wild-type Sus scrofa
1.17.1.4 S1227A Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) 2.5fold increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) 2fold increased compared to wild-type, Vmax (xanthine) 2fold decreased to wild-type Sus scrofa
1.17.1.4 S360P Km (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) increased compared to wild-type, Vmax (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) decreased compared to wild-type, Km (xanthine) 3fold increased compared to wild-type, Vmax (xanthine) 3fold decreased compared to wild-type Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.4 additional information
-
 ? KM (mM) (cyanoacetylhydrazone 2-formylquinoxaline-1,4-dioxide) (pH 7.4, 37°C), wild-type: 0.0582, mutant G47A: 0.06568, mutant N352A: 0.0601, mutant S360P: 0.09353, mutant R427E: 0.02086, mutant D430H: 0.06468, mutant D431A: 0.06129, mutant S1227A: 0.148, mutant K1230A: 0.162 Sus scrofa
1.17.1.4 0.0213
-
 xanthine pH 7.4, 37°C, mutant D430H, Vmax (micromol/min/mg): 7.47 Sus scrofa
1.17.1.4 0.02169
-
 xanthine pH 7.4, 37°C, wild-type, Vmax (micromol/min/mg): 7.74 Sus scrofa
1.17.1.4 0.02172
-
 xanthine pH 7.4, 37°C, mutant D431A, Vmax (micromol/min/mg): 8.52 Sus scrofa
1.17.1.4 0.02227
-
 xanthine pH 7.4, 37°C, mutant N352A, Vmax (micromol/min/mg): 7.69 Sus scrofa
1.17.1.4 0.02324
-
 xanthine pH 7.4, 37°C, mutant R427E, Vmax (micromol/min/mg): 7.75 Sus scrofa
1.17.1.4 0.02413
-
 xanthine pH 7.4, 37°C, mutant G47A, Vmax (micromol/min/mg): 7.91 Sus scrofa
1.17.1.4 0.039
-
 xanthine pH 7.4, 37°C, mutant K1230A, Vmax (micromol/min/mg): 4.02 Sus scrofa
1.17.1.4 0.0508
-
 xanthine pH 7.4, 37°C, mutant S1227A, Vmax (micromol/min/mg): 3.94 Sus scrofa
1.17.1.4 0.06428
-
 xanthine pH 7.4, 37°C, mutant S360P, Vmax (micromol/min/mg): 2.83 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 150000
-
-
 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 additional information in the presence of xanthine and NAD+ the enzyme catalyses the oxidation of cyadox (2-cyano-N'-((E)-1,4-dioxido-2-quinazolinyl)methylene)acetohydrazide to the respective N-oxide Sus scrofa ?
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Sus scrofa urate + NADH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.1.4 xanthine oxidoreductase
-
 Sus scrofa
1.17.1.4 XOR
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.4 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.4 7.4
-
 assay at Sus scrofa