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Literature summary extracted from

  • Munshi, T.; Gupta, A.; Evangelopoulos, D.; Guzman, J.D.; Gibbons, S.; Keep, N.H.; Bhakta, S.
    Characterisation of ATP-dependent Mur ligases involved in the biogenesis of cell wall peptidoglycan in Mycobacterium tuberculosis (2013), PLoS ONE, 8, e60143.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.8 gene murC, located in the division/cell wall (dcw) cluster, identification of a promoter driving the co-transcription of mur synthetases along with key cell division genes such as ftsQ and ftsW, recombinant expression of His6-tagged enzyme in Escherichia coli BL21(DE3)/pLysS and Pseudomonas putida KT2442, subcloning in Escherichia coli strain DH5alpha. Coexpression of genes murC/D/E/F/nat and genes pknA, pknB, murI, dapF, ddlA, namH, Rv2160c, ftsW, ftsQ, ftsZ, sepF, wag31 in Mycobacterium smegmatis Mycobacterium tuberculosis
6.3.2.13 gene murE or Rv2158c, located in the division/cell wall (dcw) cluster,, identification of a promoter driving the co-transcription of mur synthetases along with key cell division genes such as ftsQ and ftsW, recombinant expression of His6-tagged enzyme in Escherichia coli BL21(DE3)/pLysS and Pseudomonas putida KT2442, subcloning in Escherichia coli strain DH5alpha.. Coexpression of genes murC/D/E/F/nat and genes pknA, pknB, murI, dapF, ddlA, namH, Rv2160c, ftsW, ftsQ, ftsZ, sepF, wag31 in Mycobacterium smegmatis Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.8 0.0235
-
 UDP-N-acetylmuramate pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis
6.3.2.8 0.043
-
 L-alanine pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis
6.3.2.8 0.0677
-
 ATP pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis
6.3.2.8 0.0997
-
 L-serine pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis
6.3.2.8 0.1465
-
 glycine pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.8 cell wall
-
 Mycobacterium tuberculosis 5618
-
6.3.2.13 cell wall
-
 Mycobacterium tuberculosis 5618
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.8 Co2+ can partially substitute for Mg2+ Mycobacterium tuberculosis
6.3.2.8 Mg2+ required Mycobacterium tuberculosis
6.3.2.8 Mn2+ can substitute for Mg2+ Mycobacterium tuberculosis
6.3.2.8 Zn2+ can partially substitute for Mg2+ Mycobacterium tuberculosis
6.3.2.13 Co2+ can partially substitute for Mg2+ Mycobacterium tuberculosis
6.3.2.13 Mg2+ required Mycobacterium tuberculosis
6.3.2.13 Mn2+ can substitute for Mg2+ Mycobacterium tuberculosis
6.3.2.13 Zn2+ can partially substitute for Mg2+ Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.8 ATP + UDP-N-acetylmuramate + L-alanine Mycobacterium tuberculosis
-
 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-alanine Mycobacterium tuberculosis H37Rv
-
 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
 ?
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate Mycobacterium tuberculosis
-
 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate Mycobacterium tuberculosis H37Rv
-
 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?
6.3.2.13 additional information Mycobacterium tuberculosis MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP ?
-
 ?
6.3.2.13 additional information Mycobacterium tuberculosis H37Rv MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.8 Mycobacterium tuberculosis
-
 gene murC
-
6.3.2.8 Mycobacterium tuberculosis H37Rv
-
 gene murC
-
6.3.2.13 Mycobacterium tuberculosis P9WJL3 gene murE or Rv2158c
-
6.3.2.13 Mycobacterium tuberculosis H37Rv P9WJL3 gene murE or Rv2158c
-

Storage Stability

EC Number Storage Stability Organism
6.3.2.8 25°C, purified recombinant His-tagged enzyme, 50% loss of activity, 1 week Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.8 ATP + UDP-N-acetylmuramate + glycine
-
 Mycobacterium tuberculosis ADP + phosphate + UDP-N-acetylmuramoyl-glycine
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + glycine
-
 Mycobacterium tuberculosis H37Rv ADP + phosphate + UDP-N-acetylmuramoyl-glycine
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-alanine
-
 Mycobacterium tuberculosis ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-alanine
-
 Mycobacterium tuberculosis H37Rv ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-serine
-
 Mycobacterium tuberculosis ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
 ?
6.3.2.8 ATP + UDP-N-acetylmuramate + L-serine
-
 Mycobacterium tuberculosis H37Rv ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
 ?
6.3.2.8 additional information MurC is able to incorporate glycine and L-Ala to UDP-MurNAc in Mycobacterium tuberculosis, the enzyme is highly specific for ATP and UDP-N-acetylmuramate, poor activity with ADP, GTP, CTP, UTP, and dTTP Mycobacterium tuberculosis ?
-
 ?
6.3.2.8 additional information MurC is able to incorporate glycine and L-Ala to UDP-MurNAc in Mycobacterium tuberculosis, the enzyme is highly specific for ATP and UDP-N-acetylmuramate, poor activity with ADP, GTP, CTP, UTP, and dTTP Mycobacterium tuberculosis H37Rv ?
-
 ?
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
-
 Mycobacterium tuberculosis ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
-
 Mycobacterium tuberculosis H37Rv ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
-
 ?
6.3.2.13 additional information MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP Mycobacterium tuberculosis ?
-
 ?
6.3.2.13 additional information MurE is only active in the presence of its specific natural substrates UDP-N-acetylmuramoyl-L-alanine-D-glutamate, meso-2,6-diaminoheptanedioate, and ATP Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.8 Mur ligase
-
 Mycobacterium tuberculosis
6.3.2.8 MurC
-
 Mycobacterium tuberculosis
6.3.2.8 MurC synthetase
-
 Mycobacterium tuberculosis
6.3.2.13 Mur ligase
-
 Mycobacterium tuberculosis
6.3.2.13 MurE
-
 Mycobacterium tuberculosis
6.3.2.13 MurE synthetase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.8 35 40
-
 Mycobacterium tuberculosis
6.3.2.13 35 40
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.8 10 70 purified recombinant His-tagged enzyme, stable up to 40°C, MurC activity diminishes at 45-70°C Mycobacterium tuberculosis
6.3.2.13 10 40 purified recombinant His-tagged enzyme, stable up to Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.8 1
-
 L-alanine pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis
6.3.2.8 1.2
-
 L-serine pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis
6.3.2.8 1.53
-
 glycine pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.8 8.5
-
-
 Mycobacterium tuberculosis
6.3.2.13 8.5
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.8 ATP
-
 Mycobacterium tuberculosis
6.3.2.13 ATP
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
6.3.2.8 additional information protein-protein interaction network of MurC, D, E, and F synthetases, overview Mycobacterium tuberculosis
6.3.2.8 physiological function ATP-dependent Mur ligases play essential roles in the biosynthesis of cell wall peptidoglycan as they catalyze the ligation of key amino acid residues to the stem peptide at the expense of ATP hydrolysis Mycobacterium tuberculosis
6.3.2.13 additional information protein-protein interaction network of MurC, D, E, and F synthetases, overview Mycobacterium tuberculosis
6.3.2.13 physiological function ATP-dependent Mur ligases play essential roles in the biosynthesis of cell wall peptidoglycan as they catalyze the ligation of key amino acid residues to the stem peptide at the expense of ATP hydrolysis Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3.2.8 10.47
-
 glycine pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis
6.3.2.8 12.5
-
 L-serine pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis
6.3.2.8 15.1
-
 ATP pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis
6.3.2.8 23.9
-
 L-alanine pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis
6.3.2.8 44.8
-
 UDP-N-acetylmuramate pH 8.5, 37°C, recombinant enzyme Mycobacterium tuberculosis