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Literature summary extracted from

  • Snajder, M.; Vilfan, T.; Cernilec, M.; Rupreht, R.; Popovic, M.; Juntes, P.; Serbec, V.C., Ulrih, N.P.
    Enzymatic degradation of PrPSc by a protease secreted from Aeropyrum pernix K1 (2012), PLoS One, 7, e39548.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B57 expression in Escherichia coli Aeropyrum pernix

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B57 2-mercaptoethanol 5 mM, 61% loss of activity in absence of CaCl2 Aeropyrum pernix
3.4.21.B57 dithiothreitol 5 mM, 64% loss of activity in absence of CaCl2 Aeropyrum pernix
3.4.21.B57 EDTA 5 mM, complete loss of activity Aeropyrum pernix
3.4.21.B57 EGTA 5 mM, complete loss of activity Aeropyrum pernix
3.4.21.B57 Guanidine HCl 5 mM, 65% loss of activity in absence of CaCl2, activation in presence of 1 mM CaCl2 Aeropyrum pernix
3.4.21.B57 PMSF 5 mM, complete loss of activity Aeropyrum pernix
3.4.21.B57 SDS 3%, 80% loss of activity in absence of CaCl2 Aeropyrum pernix
3.4.21.B57 Urea 5 mM, 50% loss of activity in absence of CaCl2 Aeropyrum pernix

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.B57 extracellular
-
 Aeropyrum pernix
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.B57 CaCl2 maximum enhancement of the purified pernisine activity is observed at around 1 mM CaCl2. Further increasing the CaCl2 above 1 mM leads to a gradual decline in this enhanced activity. In the presence of 1 mM CaCl2, the proteolytic activity of pernisine is enhanced, to reach around 75% greater activity, with the maximum activity at 105.0°C Aeropyrum pernix
3.4.21.B57 additional information NaCl and CaCl2 together do not show any cumulative effects, as they appear to separately affect the activity Aeropyrum pernix
3.4.21.B57 NaCl the maximal activity of the purified pernisine seen for 20 mM NaCl in the absence of 1 mM CaCl2 increases to more than 2fold after the addition of CaCl2, with even larger relative enhancement by CaCl2 at higher NaCl concentrations Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B57 23000
-
 x * 23000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms Aeropyrum pernix
3.4.21.B57 34000
-
 x * 34000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B57 Aeropyrum pernix
-
-
-
3.4.21.B57 Aeropyrum pernix DSM 11879
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.B57 proteolytic modification the purified pernisine has a proregion that is autocleaved during maturation Aeropyrum pernix

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B57
-
 Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B57 abnormal prion protein PrP(Sc) + H2O the prion protein PrP(Sc) is a pathological prion protein PrP isoform. The proteolytic activity of pernisine does not depend on the species of origin of the PrP used (bovine, mouse, human) Aeropyrum pernix ?
-
 ?
3.4.21.B57 abnormal prion protein PrP(Sc) + H2O the prion protein PrP(Sc) is a pathological prion protein PrP isoform. The proteolytic activity of pernisine does not depend on the species of origin of the PrP used (bovine, mouse, human) Aeropyrum pernix DSM 11879 ?
-
 ?
3.4.21.B57 azocasein + H2O
-
 Aeropyrum pernix ?
-
 ?
3.4.21.B57 azocasein + H2O
-
 Aeropyrum pernix DSM 11879 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.B57 ? x * 23000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms Aeropyrum pernix
3.4.21.B57 ? x * 34000, SDS-PAGE, the purified pernisine is in active mature form at 23 kDa and in pre-form at 34 kDa, it appears that the purified pernisin is a mixture of two different active forms Aeropyrum pernix

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B57 85
-
 substrate: azocasein, in absence of CaCl2 Aeropyrum pernix
3.4.21.B57 105
-
 substrate: azocasein, in the presence of 1 mM CaCl2 Aeropyrum pernix

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.B57 58 99 active between 58 °C and 99 °C Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.B57 70
-
 20 min, stable in presence of CaCls, about 60% loss of activity in absence of CaCl2 Aeropyrum pernix
3.4.21.B57 90
-
 20 min, stable in presence of CaCl2, about 80% loss of activity in absence of CaCl2 Aeropyrum pernix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B57 6.5
-
 in the presence of 1 mM CaCl2, at 92°C Aeropyrum pernix
3.4.21.B57 7
-
 in absence of CaCl2, at 92°C Aeropyrum pernix

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.B57 3 10 active between pH 3.0 and pH 10.0, in the presence of 1 mM CaCl2, at 92°C Aeropyrum pernix
3.4.21.B57 3.5 8 active between pH 3.5 and pH 8.0, in absence of CaCl2, at 92°C Aeropyrum pernix