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Literature summary extracted from

  • De Ingeniis, J.; Kazanov, M.D.; Shatalin, K.; Gelfand, M.S.; Osterman, A.L.; Sorci, L.
    Glutamine versus ammonia utilization in the NAD synthetase family (2012), PLoS One, 7, e39115.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.5
-
Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.5 0.13
-
deamido-NAD+ pH 7.5, 37°C Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + NH3 Methanocaldococcus jannaschii last step of NAD synthesis AMP + diphosphate + NAD+
-
?
6.3.1.5 ATP + deamido-NAD+ + NH3 Methanocaldococcus jannaschii DSM 2661 last step of NAD synthesis AMP + diphosphate + NAD+
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.5 Methanocaldococcus jannaschii Q58747
-
-
6.3.1.5 Methanocaldococcus jannaschii DSM 2661 Q58747
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.5
-
Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + NH3 last step of NAD synthesis Methanocaldococcus jannaschii AMP + diphosphate + NAD+
-
?
6.3.1.5 ATP + deamido-NAD+ + NH3 no activity with glutamine as the amide donor Methanocaldococcus jannaschii AMP + diphosphate + NAD+
-
?
6.3.1.5 ATP + deamido-NAD+ + NH3 last step of NAD synthesis Methanocaldococcus jannaschii DSM 2661 AMP + diphosphate + NAD+
-
?
6.3.1.5 ATP + deamido-NAD+ + NH3 no activity with glutamine as the amide donor Methanocaldococcus jannaschii DSM 2661 AMP + diphosphate + NAD+
-
?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.5 ammonia-dependent NADS
-
Methanocaldococcus jannaschii
6.3.1.5 MJ1352 locus name Methanocaldococcus jannaschii
6.3.1.5 mj_NADS
-
Methanocaldococcus jannaschii
6.3.1.5 NAD synthetase
-
Methanocaldococcus jannaschii
6.3.1.5 NadE
-
Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.1.5 0.2
-
deamido-NAD+ pH 7.5, 37°C Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
6.3.1.5 metabolism last step of NAD synthesis Methanocaldococcus jannaschii