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Literature summary extracted from

  • Liu, J.; Sun, Z.; Zhong, Y.; Huang, J.; Hu, Q.; Chen, F.
    Stearoyl-acyl carrier protein desaturase gene from the oleaginous microalga Chlorella zofingiensis: cloning, characterization and transcriptional analysis (2012), Planta, 236, 1665-1676.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.2 expressed in Escherichia coli BL21(DE3) cells Chromochloris zofingiensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.2 chloroplast
-
 Chromochloris zofingiensis 9507
-
1.14.19.2 soluble
-
 Chromochloris zofingiensis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.2 47040
-
 x * 47040, calculated from amino acid sequence Chromochloris zofingiensis

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.2 Chromochloris zofingiensis D0V0B8
-
-
1.14.19.2 Chromochloris zofingiensis ATCC 30412 D0V0B8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.2 stearoyl-[acyl-carrier protein] + reduced acceptor + O2
-
 Chromochloris zofingiensis oleoyl-[acyl-carrier protein] + acceptor + H2O
-
 ?
1.14.19.2 stearoyl-[acyl-carrier protein] + reduced acceptor + O2
-
 Chromochloris zofingiensis ATCC 30412 oleoyl-[acyl-carrier protein] + acceptor + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.2 ? x * 47040, calculated from amino acid sequence Chromochloris zofingiensis

Synonyms

EC Number Synonyms Comment Organism
1.14.19.2 SAD
-
 Chromochloris zofingiensis
1.14.19.2 stearoyl-ACP desaturase
-
 Chromochloris zofingiensis
1.14.19.2 stearoyl-acyl carrier protein desaturase
-
 Chromochloris zofingiensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.2 Ferredoxin
-
 Chromochloris zofingiensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.19.2 Chromochloris zofingiensis calculated from amino acid sequence
-
 6.4

Expression

EC Number Organism Comment Expression
1.14.19.2 Chromochloris zofingiensis exposure to HL (0.4 mM photons m-2 s-1) significantly increases the enzyme's mRNA level. Nitrogen deficiency also markedly up-regulates the expression of the enzyme. mRNA levels of the enzyme are drastically upregulated, reaching their maximum at 48 h after glucose induction up