EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.158 | gene LeLUT1, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, expression of gene LeLUT1-GFP fusion protein in chloroplasts of Arabidopsis thaliana mesophyll protoplasts, quantitative real-time PCR expression analysis | Solanum lycopersicum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.158 | additional information | construction of transgenic Nicotiana tabacum plants overexpressing LeLUT1, the transgenic plants have a higher lutein content, which is decreased in cold condition. Under chilling stress, the non-photochemical quenching values are higher in the transgenic plants than in the wild-type plants. Compared with the wild-type plants, the transgenic plants show lower levels of hydrogen peroxide, superoxide radical, relative electrical conductivity, and malondialdehyde content, and relatively higher values of maximal photochemical efficiency of photosystem II, oxidizable P700 of PSI, and net photosynthetic rate. The transgenic seedlings are less suppressed in growth and lose less cotyledon chlorophyll than the wild-type seedlings | Solanum lycopersicum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.158 | chloroplast | - |
Solanum lycopersicum | 9507 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.158 | zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 | Solanum lycopersicum | - |
lutein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.158 | Solanum lycopersicum | BT012891 | mRNA; cv. Zhongshu 4, gene LUT1 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.158 | leaf | expression of LeLUT1 is the highest in the leaves in tomato | Solanum lycopersicum | - |
1.14.14.158 | additional information | expression pattern in various tomato organs, quantitative expression analysis, overview | Solanum lycopersicum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.158 | zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Solanum lycopersicum | lutein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.158 | carotenoid epsilon-hydroxylase | - |
Solanum lycopersicum |
1.14.14.158 | carotenoid epsilon-ring hydroxylase | - |
Solanum lycopersicum |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.14.158 | Solanum lycopersicum | expression of LeLUT1 is downregulated by various abiotic stresses in tomato | down |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.158 | metabolism | the enzyme is involved in the biosynthetic pathway of lutein in plants, overview | Solanum lycopersicum |
1.14.14.158 | physiological function | the overexpression of gene LeLUT1 has a key function in alleviating photoinhibition and photooxidation, and decreases the sensitivity of photosynthesis to chilling stress | Solanum lycopersicum |