Literature summary extracted from

Ke, D.; Tu, S.C.
Activities, kinetics and emission spectra of bacterial luciferase-fluorescent protein fusion enzymes (2011), Photochem. Photobiol., 87, 1346-1353.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.14.3	expressed in Escherichia coli JM109 (native enzyme) and BL21(DE3) cells (luciferase-mOrange fusion enzyme)	Vibrio harveyi

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.14.14.3	0.00018	-	FMNH2	luciferase-mOrange fusion enzyme, at 22°C, pH not specified in the publication	Vibrio harveyi
1.14.14.3	0.0002	-	FMNH2	recombinant wild type enzyme, at 22°C, pH not specified in the publication	Vibrio harveyi
1.14.14.3	0.00113	-	decanal	recombinant wild type enzyme, at 22°C, pH not specified in the publication	Vibrio harveyi
1.14.14.3	0.00165	-	decanal	luciferase-mOrange fusion enzyme, at 22°C, pH not specified in the publication	Vibrio harveyi

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.14.3	Vibrio harveyi	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.14.14.3	DEAE-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography	Vibrio harveyi

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.14.3	decanal + FMNH2 + O2	-	Vibrio harveyi	decanoic acid + FMN + H2O + hv	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.14.3	luciferase	-	Vibrio harveyi
1.14.14.3	Lux	-	Vibrio harveyi

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Release 2023.1 (January 2023)