EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.258 | expression in Escherichia coli | Clostridioides difficile |
2.3.1.169 | expression in Escherichia coli | Clostridioides difficile |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.169 | 1,10-phenanthroline | 1 mM, complete inhibition. 1,10-Phenanthroline additionally has inhibitory effects on growth of Peptoclostridium difficile | Clostridioides difficile | |
2.3.1.169 | 2,2-dipyridyl | 1 mM, complete inhibition. 2,2-Dipyridyl additionally has inhibitory effects on growth of Peptoclostridium difficile | Clostridioides difficile |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.258 | 0.0178 | - |
Cobalamin | 25°C, pH 5.1 | Clostridioides difficile | |
2.1.1.258 | 0.06 | - |
Cobalamin | 25°C, pH 7.2 | Clostridioides difficile |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.169 | Iron | protein contains in average 3.7 Fe atoms and 1.6 Ni atoms per monomer molecule, which is consistent with the presence of a [NipNid]-[Fe4S4]-center | Clostridioides difficile | |
2.3.1.169 | Nickel | protein contains in average 3.7 Fe atoms and 1.6 Ni atoms per monomer molecule, which is consistent with the presence of a [NipNid]-[Fe4S4]-center | Clostridioides difficile |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.258 | Clostridioides difficile | - |
- |
- |
2.1.1.258 | Clostridioides difficile 630 | - |
- |
- |
2.3.1.169 | Clostridioides difficile | - |
- |
- |
2.3.1.169 | Clostridioides difficile 630 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.258 | cobalamin + 5-methyltetrahydrofolate | reaction is pH dependent with the best pH of around 5.0 | Clostridioides difficile | methylcobalamin + tetrahydrofolate | - |
? | |
2.1.1.258 | cobalamin + 5-methyltetrahydrofolate | reaction is pH dependent with the best pH of around 5.0 | Clostridioides difficile 630 | methylcobalamin + tetrahydrofolate | - |
? | |
2.3.1.169 | CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA | - |
Clostridioides difficile | CH3-CO-S-CoA + corrinoid/iron-sulfur protein | - |
? | |
2.3.1.169 | CH3-(corrinoid/iron-sulfur protein) + CO + HS-CoA | - |
Clostridioides difficile 630 | CH3-CO-S-CoA + corrinoid/iron-sulfur protein | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.258 | 2.63 | - |
Cobalamin | 25°C, pH 5.1 | Clostridioides difficile | |
2.1.1.258 | 37.2 | - |
Cobalamin | 25°C, pH 7.2 | Clostridioides difficile |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.169 | [4Fe-4S]-center | protein contains in average 3.7 Fe atoms and 1.6 Ni atoms per monomer molecule, which is consistent with the presence of a [NipNid] [Fe4S4] center. Preparation shows a broad absorption at the 420 nm region | Clostridioides difficile |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.1.1.258 | Clostridioides difficile | calculated | - |
4.8 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.258 | 150 | - |
Cobalamin | 25°C, pH 5.1 | Clostridioides difficile | |
2.1.1.258 | 620 | - |
Cobalamin | 25°C, pH 7.2 | Clostridioides difficile | |
2.3.1.169 | 0.66 | - |
HS-CoA | at 1 atm CO, pH not specified in the publication, temperature not specified in the publication | Clostridioides difficile |