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Literature summary extracted from

  • Chen, Y.; Xiao, C.; Chen, X.; Yang, L.; Qi, X.; Zheng, J.; Li, M.; Zhang, J.
    Preparation of cross-linked enzyme aggregates in water-in-oil emulsion: application to trehalose synthase (2014), J. Mol. Catal. B, 100, 84-90.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.16 recombinant expression in Escherichia coli Meiothermus ruber

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.16 additional information preparation of cross-linked enzyme aggregates in a water-in-oil emulsion, spherical aggregates of recombinant trehalose synthase are obtained through the emulsion based process, method development and evaluation Meiothermus ruber

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.16 Al3+ complete inhibition at 2 mM, 8% inhibition of aggregated enzyme Meiothermus ruber
5.4.99.16 Cu2+ complete inhibition at 2 mM, 23% inhibition of aggregated enzyme Meiothermus ruber
5.4.99.16 Fe2+ 95% inhibition at 2 mM, 5% inhibition of aggregated enzyme Meiothermus ruber
5.4.99.16 Guanidine-HCl complete inhibition at 1 M, 51% inhibition of aggregated enzyme Meiothermus ruber
5.4.99.16 Tris complete inhibition at 25 mM, 23% inhibition of aggregated enzyme Meiothermus ruber
5.4.99.16 Urea 90% inhibition at 2 M, 1% inhibition of aggregated enzyme Meiothermus ruber
5.4.99.16 Zn2+ 92% inhibition at 2 mM, 1% inhibition of aggregated enzyme Meiothermus ruber

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.16 Meiothermus ruber
-
-
-

Synonyms

EC Number Synonyms Comment Organism
5.4.99.16 Trehalose synthase
-
Meiothermus ruber
5.4.99.16 TreS
-
Meiothermus ruber

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.16 53
-
free enzyme Meiothermus ruber
5.4.99.16 70
-
spherical aggregated enzyme Meiothermus ruber

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.4.99.16 40 70 free enzyme Meiothermus ruber
5.4.99.16 50 80 spherical aggregated enzyme Meiothermus ruber

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.16 6.5
-
free enzyme Meiothermus ruber
5.4.99.16 7
-
spherical aggregated enzyme Meiothermus ruber

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.99.16 5 8 free and spherical aggregated enzyme Meiothermus ruber