EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.15 | 0.00003 | - |
tRNAPro | pH 7.0, 37°C | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.15 | ATP + L-proline + tRNAPro | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
6.1.1.15 | ATP + L-proline + tRNAPro | Methanocaldococcus jannaschii DSM 2661 | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.15 | Methanocaldococcus jannaschii | Q58635 | - |
- |
6.1.1.15 | Methanocaldococcus jannaschii DSM 2661 | Q58635 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.15 | ATP + L-proline + tRNAPro | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
6.1.1.15 | ATP + L-proline + tRNAPro | the unmodified transcript of Methanococcus jannaschii tRNAPro is mis-acylated with cysteine. The origin of mischarging is not at the anticodon or acceptor stem. Replacement of the D loop in the tRNA core with that of tRNACys suppresses mischarging with cysteine without compromising the activity of aminoacylation with proline. Prevention of mis-placement by alteration of the core structure or by nucleotide modifications in the tRNA illustrates a novel strategy of the dual-specificity synthetase | Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
6.1.1.15 | ATP + L-proline + tRNAPro | - |
Methanocaldococcus jannaschii DSM 2661 | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
6.1.1.15 | ATP + L-proline + tRNAPro | the unmodified transcript of Methanococcus jannaschii tRNAPro is mis-acylated with cysteine. The origin of mischarging is not at the anticodon or acceptor stem. Replacement of the D loop in the tRNA core with that of tRNACys suppresses mischarging with cysteine without compromising the activity of aminoacylation with proline. Prevention of mis-placement by alteration of the core structure or by nucleotide modifications in the tRNA illustrates a novel strategy of the dual-specificity synthetase | Methanocaldococcus jannaschii DSM 2661 | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.15 | 65 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.15 | 0.58 | - |
tRNAPro | pH 7.0, 37°C | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.15 | 7 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.15 | 19330 | - |
tRNAPro | pH 7.0, 37°C | Methanocaldococcus jannaschii |