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Literature summary extracted from

  • Lipman, R.S.; Wang, J.; Sowers, K.R.; Hou, Y.M.
    Prevention of mis-aminoacylation of a dual-specificity aminoacyl-tRNA synthetase (2002), J. Mol. Biol., 315, 943-999.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.15 0.00003
-
tRNAPro pH 7.0, 37°C Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro Methanocaldococcus jannaschii
-
AMP + diphosphate + L-prolyl-tRNAPro
-
?
6.1.1.15 ATP + L-proline + tRNAPro Methanocaldococcus jannaschii DSM 2661
-
AMP + diphosphate + L-prolyl-tRNAPro
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Methanocaldococcus jannaschii Q58635
-
-
6.1.1.15 Methanocaldococcus jannaschii DSM 2661 Q58635
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro
-
Methanocaldococcus jannaschii AMP + diphosphate + L-prolyl-tRNAPro
-
?
6.1.1.15 ATP + L-proline + tRNAPro the unmodified transcript of Methanococcus jannaschii tRNAPro is mis-acylated with cysteine. The origin of mischarging is not at the anticodon or acceptor stem. Replacement of the D loop in the tRNA core with that of tRNACys suppresses mischarging with cysteine without compromising the activity of aminoacylation with proline. Prevention of mis-placement by alteration of the core structure or by nucleotide modifications in the tRNA illustrates a novel strategy of the dual-specificity synthetase Methanocaldococcus jannaschii AMP + diphosphate + L-prolyl-tRNAPro
-
?
6.1.1.15 ATP + L-proline + tRNAPro
-
Methanocaldococcus jannaschii DSM 2661 AMP + diphosphate + L-prolyl-tRNAPro
-
?
6.1.1.15 ATP + L-proline + tRNAPro the unmodified transcript of Methanococcus jannaschii tRNAPro is mis-acylated with cysteine. The origin of mischarging is not at the anticodon or acceptor stem. Replacement of the D loop in the tRNA core with that of tRNACys suppresses mischarging with cysteine without compromising the activity of aminoacylation with proline. Prevention of mis-placement by alteration of the core structure or by nucleotide modifications in the tRNA illustrates a novel strategy of the dual-specificity synthetase Methanocaldococcus jannaschii DSM 2661 AMP + diphosphate + L-prolyl-tRNAPro
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.15 65
-
assay at Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.15 0.58
-
tRNAPro pH 7.0, 37°C Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.15 7
-
assay at Methanocaldococcus jannaschii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.15 19330
-
tRNAPro pH 7.0, 37°C Methanocaldococcus jannaschii