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Literature summary extracted from

  • Chodok, P.; Eiamsa-ard, P.; Cove, D.J.; Quatrano, R.S.; Kaewsuwan, S.
    Identification and functional characterization of two DELTA12-fatty acid desaturases associated with essential linoleic acid biosynthesis in Physcomitrella patens (2013), J. Ind. Microbiol. Biotechnol., 40, 901-913.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.39 gene PpFAD2-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Saccharomyces cerevisiae strain INVSc1 Physcomitrium patens
1.14.19.39 genes PpFAD2-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Saccharomyces cerevisiae strain INVSc1 Physcomitrium patens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.39 endoplasmic reticulum membrane the transmembrane protein PpFAD2-1 contains three conserved histidine-rich motifs: the HXXXH motif from amino acid residue 99 to 103 (HECGH), the HXXHH motif from amino acid residue 135 to 139 (HRRHH), and the HXXHH motif from amino acid residue 309 to 313 (HVAHH) in the C-terminus Physcomitrium patens 5789
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1.14.19.39 endoplasmic reticulum membrane the transmembrane protein PpFAD2-2 contains three conserved histidine-rich motifs: the HXXXH motif from amino acid residue 99 to 103 (HECGH), the HXXHH motif from amino acid residue 135 to 139 (HRRHH), and the HXXHH motif from amino acid residue 309 to 313 (HVAHH) in the C-terminus Physcomitrium patens 5789
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1.14.19.39 microsome
-
 Physcomitrium patens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.39 phosphatidylcholine + reduced cytochrome b5 + O2 Physcomitrium patens with NADH ? + cytochrome b5 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.39 Physcomitrium patens A9RQ27 FAD2-2; gene PpFAD2-2
-
1.14.19.39 Physcomitrium patens A9RV06 FAD2-1; gene PpFAD2-1 encoding two isozymes
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.39 phosphatidylcholine + reduced cytochrome b5 + O2 with NADH Physcomitrium patens ? + cytochrome b5 + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.39 DELTA12-fatty acid desaturases
-
 Physcomitrium patens
1.14.19.39 FAD2
-
 Physcomitrium patens
1.14.19.39 FAD2-1
-
 Physcomitrium patens
1.14.19.39 FAD2-2
-
 Physcomitrium patens
1.14.19.39 microsomal DELTA12-fatty acid desaturase
-
 Physcomitrium patens

General Information

EC Number General Information Comment Organism
1.14.19.39 evolution the microsomal DELTA12-fatty acid desaturase (FAD2) is located in the endoplasmic reticulum and uses phosphatidylcholine as acyl substrate and NADH, NADH-cytochrome b5 reductase, and cytochrome b5 as electron donors. This reaction involves concomitant reduction of molecular oxygen to water. In contrast, the plastidial DELTA12-fatty acid desaturase (FAD6) is located in the chloroplast and uses primarily glycolipids as acyl carriers and NAD(P)H, ferredoxin-NAD(P) reductase, and ferredoxin as electron donors Physcomitrium patens
1.14.19.39 additional information the isozyme contains the three histidine clusters typical of the catalytic domains of DELTA12-desaturase enzymes Physcomitrium patens