EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.3 | expressed in Escherichia coli BL21(DE3) and HEK-293T cells | Vibrio campbellii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.3 | 77000 | - |
2 * 77000, SDS-PAGE | Vibrio campbellii |
1.14.14.3 | 77800 | - |
2 * 77800, calculated from amino acid sequence | Vibrio campbellii |
1.14.14.3 | 158000 | - |
gel filtration | Vibrio campbellii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.3 | Vibrio campbellii | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.3 | RCHO + FMNH2 + O2 | - |
Vibrio campbellii | RCOOH + FMN + H2O + hv | the enzyme is emitting blue-green light at 490 nm | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.3 | homodimer | 2 * 77000, SDS-PAGE | Vibrio campbellii |
1.14.14.3 | homodimer | 2 * 77800, calculated from amino acid sequence | Vibrio campbellii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.3 | luciferase | - |
Vibrio campbellii |
1.14.14.3 | Lux | - |
Vibrio campbellii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.3 | 30 | 37 | half-life of about 1020 min at 37°C. At 37°C, the native enzyme retains around 60% of the bioluminescence of the enzyme expressed at 30°C | Vibrio campbellii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.3 | 7 | - |
- |
Vibrio campbellii |