EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.1 | expressed in Escherichia coli | Aeropyrum pernix |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.1 | 0.0004 | - |
NADH | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.001 | - |
NAD+ | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.131 | - |
4-methoxyphenylacetone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.147 | - |
1-Hexanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.147 | - |
2-decanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.215 | - |
2-nonanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.286 | - |
2-Octanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.333 | - |
benzaldehyde | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.396 | - |
1-Pentanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.596 | - |
1-butanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.694 | - |
tert-butyl acetoacetate | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.703 | - |
Cyclohexanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.752 | - |
2-Pentanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 1.03 | - |
1-propanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 1.05 | - |
2-butanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 1.13 | - |
4-methoxybenzyl alcohol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 1.16 | - |
2-Heptanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 1.39 | - |
cyclohexanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 2.44 | - |
2-propanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 5.01 | - |
2-Hexanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 5.15 | - |
2-Pentanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 5.43 | - |
benzyl alcohol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 13.7 | - |
ethanol | pH 8.0, 60°C | Aeropyrum pernix |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.1 | 39570 | - |
4 * 39570, calculated from sequence | Aeropyrum pernix |
1.1.1.1 | 40000 | - |
4 * 40000, SDS-PAGE | Aeropyrum pernix |
1.1.1.1 | 160000 | - |
gel filtration | Aeropyrum pernix |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.1 | Aeropyrum pernix | Q9Y9P9 | - |
- |
1.1.1.1 | Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 | Q9Y9P9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.1 | - |
Aeropyrum pernix |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.1 | 1-butanol + NAD+ | - |
Aeropyrum pernix | butanal + NADH + H+ | - |
? | |
1.1.1.1 | 1-butanol + NAD+ | - |
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 | butanal + NADH + H+ | - |
? | |
1.1.1.1 | 1-hexanol + NAD+ | - |
Aeropyrum pernix | 1-hexanal + NADH + H+ | - |
? | |
1.1.1.1 | 1-pentanol + NAD+ | - |
Aeropyrum pernix | 1-pentanal + NADH + H+ | - |
? | |
1.1.1.1 | 1-propanol + NAD+ | - |
Aeropyrum pernix | propanal + NADH + H+ | - |
? | |
1.1.1.1 | 1-propanol + NAD+ | - |
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 | propanal + NADH + H+ | - |
? | |
1.1.1.1 | 2-butanol + NAD+ | - |
Aeropyrum pernix | 2-butanone + NADH + H+ | - |
? | |
1.1.1.1 | 2-butanol + NAD+ | - |
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 | 2-butanone + NADH + H+ | - |
? | |
1.1.1.1 | 2-decanone + NADH | - |
Aeropyrum pernix | (S)-2-decanol + NAD+ + H+ | 92% enantiomeric excess | ? | |
1.1.1.1 | 2-heptanone + NADH | - |
Aeropyrum pernix | (S)-2-heptanol + NAD+ + H+ | 79% enantiomeric excess | ? | |
1.1.1.1 | 2-hexanone + NADH | - |
Aeropyrum pernix | (S)-2-hexanol + NAD+ + H+ | 37% enantiomeric excess | ? | |
1.1.1.1 | 2-nonanone + NADH | - |
Aeropyrum pernix | (S)-2-nonanol + NAD+ + H+ | 95% enantiomeric excess | ? | |
1.1.1.1 | 2-octanone + NADH | - |
Aeropyrum pernix | (S)-2-octanol + NAD+ + H+ | 92% enantiomeric excess | ? | |
1.1.1.1 | 2-pentanol + NAD+ | - |
Aeropyrum pernix | 2-pentanone + NADH + H+ | - |
? | |
1.1.1.1 | 2-pentanone + NADH + H+ | - |
Aeropyrum pernix | (S)-2-pentanol + NAD+ | 60% enantiomeric excess | ? | |
1.1.1.1 | 2-propanol + NAD+ | - |
Aeropyrum pernix | acetone + NADH + H+ | - |
? | |
1.1.1.1 | 2-propanol + NAD+ | - |
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 | acetone + NADH + H+ | - |
? | |
1.1.1.1 | 4-methoxybenzyl alcohol + NAD+ | - |
Aeropyrum pernix | 4-methoxybenzaldehyde + NADH + H+ | - |
? | |
1.1.1.1 | 4-methoxyphenylacetone + NADH | - |
Aeropyrum pernix | (2S)-1-(4-methoxyphenyl)propan-2-ol + NAD+ + H+ | - |
? | |
1.1.1.1 | benzyl alcohol + NAD+ | i.e. phenylmethanol | Aeropyrum pernix | benzaldehyde + NADH + H+ | - |
? | |
1.1.1.1 | cyclohexanol + NAD+ | - |
Aeropyrum pernix | cyclohexanone + NADH + H+ | - |
? | |
1.1.1.1 | ethanol + NAD+ | - |
Aeropyrum pernix | acetaldehyde + NADH + H+ | - |
? | |
1.1.1.1 | additional information | the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone | Aeropyrum pernix | ? | - |
? | |
1.1.1.1 | additional information | the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone | Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 | ? | - |
? | |
1.1.1.1 | tert-butyl acetoacetate + NADH + H+ | - |
Aeropyrum pernix | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.1 | homotetramer | 4 * 40000, SDS-PAGE | Aeropyrum pernix |
1.1.1.1 | homotetramer | 4 * 39570, calculated from sequence | Aeropyrum pernix |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.1 | APE2239 | - |
Aeropyrum pernix |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.1 | 95 | - |
catalytic activity increased up to 95°C | Aeropyrum pernix |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.1 | 70 | - |
30 min, no loss of activity | Aeropyrum pernix |
1.1.1.1 | 80 | - |
30 min, about 20% loss of activity | Aeropyrum pernix |
1.1.1.1 | 98 | - |
the enzyme maintains 24% of the original catalytic activity after incubation for 30 min | Aeropyrum pernix |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.1 | 0.071 | - |
4-methoxyphenylacetone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.072 | - |
tert-butyl acetoacetate | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.23 | - |
ethanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.24 | - |
2-propanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.26 | - |
1-propanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.37 | - |
1-Hexanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.4 | - |
NAD+ | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.4 | - |
2-decanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.41 | - |
NADH | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.41 | - |
1-butanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.45 | - |
1-Pentanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.48 | - |
2-butanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.52 | - |
Cyclohexanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.6 | - |
4-methoxybenzyl alcohol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.6 | - |
2-Pentanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.7 | 1 | 2-nonanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.73 | - |
2-Heptanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.74 | - |
2-Octanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.77 | - |
2-Pentanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 1.02 | - |
benzyl alcohol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 1.08 | - |
2-Hexanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 1.22 | - |
benzaldehyde | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 1.27 | - |
cyclohexanone | pH 8.0, 60°C | Aeropyrum pernix |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.1 | 8 | - |
reduction of 2-pentanone | Aeropyrum pernix |
1.1.1.1 | 10.5 | - |
oxidation of 2-pentanol | Aeropyrum pernix |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.1 | 7 | 9.6 | pH 7.0: about 70% of maximal activity, pH 9.6: about 50% of maximal activity, reduction of 2-pentanone | Aeropyrum pernix |
1.1.1.1 | 9.6 | 11.5 | pH 9.5: about 40% of maximal activity, pH 11.5: about 30% of maximal activity, oxidation of 2-pentanol | Aeropyrum pernix |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.1 | 0.017 | - |
ethanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.097 | - |
2-propanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.1 | - |
tert-butyl acetoacetate | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.15 | - |
2-Pentanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.189 | - |
benzyl alcohol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.22 | - |
2-Hexanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.25 | - |
1-propanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.46 | - |
2-butanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.53 | - |
4-methoxybenzyl alcohol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.54 | - |
4-methoxyphenylacetone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.62 | - |
2-Heptanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.69 | - |
1-butanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.73 | - |
Cyclohexanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.79 | - |
2-Pentanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 0.91 | - |
cyclohexanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 1.1 | - |
1-Pentanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 2.5 | - |
1-Hexanol | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 2.6 | - |
2-Octanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 2.7 | - |
2-decanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 3.3 | - |
2-nonanone | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 3.66 | - |
benzaldehyde | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 380 | - |
NAD+ | pH 8.0, 60°C | Aeropyrum pernix | |
1.1.1.1 | 1000 | - |
NADH | pH 8.0, 60°C | Aeropyrum pernix |