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Literature summary extracted from

  • Hirakawa, H.; Kamiya, N.; Kawarabayashi, Y.; Nagamune, T.
    Properties of an alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix K1 (2004), J. Biosci. Bioeng., 97, 202-206.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expressed in Escherichia coli Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.0004
-
 NADH pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.001
-
 NAD+ pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.131
-
 4-methoxyphenylacetone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.147
-
 1-Hexanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.147
-
 2-decanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.215
-
 2-nonanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.286
-
 2-Octanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.333
-
 benzaldehyde pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.396
-
 1-Pentanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.596
-
 1-butanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.694
-
 tert-butyl acetoacetate pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.703
-
 Cyclohexanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.752
-
 2-Pentanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 1.03
-
 1-propanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 1.05
-
 2-butanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 1.13
-
 4-methoxybenzyl alcohol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 1.16
-
 2-Heptanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 1.39
-
 cyclohexanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 2.44
-
 2-propanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 5.01
-
 2-Hexanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 5.15
-
 2-Pentanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 5.43
-
 benzyl alcohol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 13.7
-
 ethanol pH 8.0, 60°C Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 39570
-
 4 * 39570, calculated from sequence Aeropyrum pernix
1.1.1.1 40000
-
 4 * 40000, SDS-PAGE Aeropyrum pernix
1.1.1.1 160000
-
 gel filtration Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Aeropyrum pernix Q9Y9P9
-
-
1.1.1.1 Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 Q9Y9P9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1-butanol + NAD+
-
 Aeropyrum pernix butanal + NADH + H+
-
 ?
1.1.1.1 1-butanol + NAD+
-
 Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 butanal + NADH + H+
-
 ?
1.1.1.1 1-hexanol + NAD+
-
 Aeropyrum pernix 1-hexanal + NADH + H+
-
 ?
1.1.1.1 1-pentanol + NAD+
-
 Aeropyrum pernix 1-pentanal + NADH + H+
-
 ?
1.1.1.1 1-propanol + NAD+
-
 Aeropyrum pernix propanal + NADH + H+
-
 ?
1.1.1.1 1-propanol + NAD+
-
 Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 propanal + NADH + H+
-
 ?
1.1.1.1 2-butanol + NAD+
-
 Aeropyrum pernix 2-butanone + NADH + H+
-
 ?
1.1.1.1 2-butanol + NAD+
-
 Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 2-butanone + NADH + H+
-
 ?
1.1.1.1 2-decanone + NADH
-
 Aeropyrum pernix (S)-2-decanol + NAD+ + H+ 92% enantiomeric excess ?
1.1.1.1 2-heptanone + NADH
-
 Aeropyrum pernix (S)-2-heptanol + NAD+ + H+ 79% enantiomeric excess ?
1.1.1.1 2-hexanone + NADH
-
 Aeropyrum pernix (S)-2-hexanol + NAD+ + H+ 37% enantiomeric excess ?
1.1.1.1 2-nonanone + NADH
-
 Aeropyrum pernix (S)-2-nonanol + NAD+ + H+ 95% enantiomeric excess ?
1.1.1.1 2-octanone + NADH
-
 Aeropyrum pernix (S)-2-octanol + NAD+ + H+ 92% enantiomeric excess ?
1.1.1.1 2-pentanol + NAD+
-
 Aeropyrum pernix 2-pentanone + NADH + H+
-
 ?
1.1.1.1 2-pentanone + NADH + H+
-
 Aeropyrum pernix (S)-2-pentanol + NAD+ 60% enantiomeric excess ?
1.1.1.1 2-propanol + NAD+
-
 Aeropyrum pernix acetone + NADH + H+
-
 ?
1.1.1.1 2-propanol + NAD+
-
 Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 acetone + NADH + H+
-
 ?
1.1.1.1 4-methoxybenzyl alcohol + NAD+
-
 Aeropyrum pernix 4-methoxybenzaldehyde + NADH + H+
-
 ?
1.1.1.1 4-methoxyphenylacetone + NADH
-
 Aeropyrum pernix (2S)-1-(4-methoxyphenyl)propan-2-ol + NAD+ + H+
-
 ?
1.1.1.1 benzyl alcohol + NAD+ i.e. phenylmethanol Aeropyrum pernix benzaldehyde + NADH + H+
-
 ?
1.1.1.1 cyclohexanol + NAD+
-
 Aeropyrum pernix cyclohexanone + NADH + H+
-
 ?
1.1.1.1 ethanol + NAD+
-
 Aeropyrum pernix acetaldehyde + NADH + H+
-
 ?
1.1.1.1 additional information the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone Aeropyrum pernix ?
-
 ?
1.1.1.1 additional information the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 ?
-
 ?
1.1.1.1 tert-butyl acetoacetate + NADH + H+
-
 Aeropyrum pernix ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 homotetramer 4 * 40000, SDS-PAGE Aeropyrum pernix
1.1.1.1 homotetramer 4 * 39570, calculated from sequence Aeropyrum pernix

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 APE2239
-
 Aeropyrum pernix

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 95
-
 catalytic activity increased up to 95°C Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 70
-
 30 min, no loss of activity Aeropyrum pernix
1.1.1.1 80
-
 30 min, about 20% loss of activity Aeropyrum pernix
1.1.1.1 98
-
 the enzyme maintains 24% of the original catalytic activity after incubation for 30 min Aeropyrum pernix

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 0.071
-
 4-methoxyphenylacetone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.072
-
 tert-butyl acetoacetate pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.23
-
 ethanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.24
-
 2-propanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.26
-
 1-propanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.37
-
 1-Hexanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.4
-
 NAD+ pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.4
-
 2-decanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.41
-
 NADH pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.41
-
 1-butanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.45
-
 1-Pentanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.48
-
 2-butanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.52
-
 Cyclohexanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.6
-
 4-methoxybenzyl alcohol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.6
-
 2-Pentanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.7 1 2-nonanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.73
-
 2-Heptanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.74
-
 2-Octanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.77
-
 2-Pentanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 1.02
-
 benzyl alcohol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 1.08
-
 2-Hexanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 1.22
-
 benzaldehyde pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 1.27
-
 cyclohexanone pH 8.0, 60°C Aeropyrum pernix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 8
-
 reduction of 2-pentanone Aeropyrum pernix
1.1.1.1 10.5
-
 oxidation of 2-pentanol Aeropyrum pernix

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 7 9.6 pH 7.0: about 70% of maximal activity, pH 9.6: about 50% of maximal activity, reduction of 2-pentanone Aeropyrum pernix
1.1.1.1 9.6 11.5 pH 9.5: about 40% of maximal activity, pH 11.5: about 30% of maximal activity, oxidation of 2-pentanol Aeropyrum pernix

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.1 0.017
-
 ethanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.097
-
 2-propanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.1
-
 tert-butyl acetoacetate pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.15
-
 2-Pentanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.189
-
 benzyl alcohol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.22
-
 2-Hexanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.25
-
 1-propanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.46
-
 2-butanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.53
-
 4-methoxybenzyl alcohol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.54
-
 4-methoxyphenylacetone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.62
-
 2-Heptanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.69
-
 1-butanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.73
-
 Cyclohexanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.79
-
 2-Pentanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 0.91
-
 cyclohexanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 1.1
-
 1-Pentanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 2.5
-
 1-Hexanol pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 2.6
-
 2-Octanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 2.7
-
 2-decanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 3.3
-
 2-nonanone pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 3.66
-
 benzaldehyde pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 380
-
 NAD+ pH 8.0, 60°C Aeropyrum pernix
1.1.1.1 1000
-
 NADH pH 8.0, 60°C Aeropyrum pernix