BRENDA - Enzyme Database show

Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506

Hirota, R.; Yamane, S.T.; Fujibuchi, T.; Motomura, K.; Ishida, T.; Ikeda, T.; Kuroda, A.; J. Biosci. Bioeng. 113, 445-450 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.20.1.1
expression in Escherichia coli
Pseudomonas stutzeri
1.20.1.1
expression in Escherichia coli
Ralstonia sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.20.1.1
arsenite
4 mM, 90% residual activity
Pseudomonas stutzeri
1.20.1.1
additional information
not inhibitory: arsenite, NaCl
Ralstonia sp.
1.20.1.1
NaCl
4 mM, 87% residual activity
Pseudomonas stutzeri
1.20.1.1
nitrate
4 mM, 21% residual activity
Pseudomonas stutzeri
1.20.1.1
nitrate
4 mM, 40% residual activity
Ralstonia sp.
1.20.1.1
sulfite
4 mM, 16% residual activity
Pseudomonas stutzeri
1.20.1.1
sulfite
4 mM, 56% residual activity
Ralstonia sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.1.1
0.0026
-
NAD+
pH 7.2, 30°C
Ralstonia sp.
1.20.1.1
0.0073
-
phosphonate
pH 7.2, 30°C
Pseudomonas stutzeri
1.20.1.1
0.0087
-
phosphonate
pH 7.2, 30°C
Ralstonia sp.
1.20.1.1
0.016
-
NAD+
pH 7.2, 40°C
Ralstonia sp.
1.20.1.1
0.024
-
phosphonate
pH 7.2, 40°C
Ralstonia sp.
1.20.1.1
0.056
-
NAD+
pH 7.2, 30°C
Pseudomonas stutzeri
1.20.1.1
0.128
-
NAD+
pH 7.2, 40°C
Pseudomonas stutzeri
1.20.1.1
0.287
-
phosphonate
pH 7.2, 40°C
Pseudomonas stutzeri
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.20.1.1
soluble
recombinant protein
Ralstonia sp.
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.1.1
Pseudomonas stutzeri
-
-
-
1.20.1.1
Pseudomonas stutzeri WM88
-
-
-
1.20.1.1
Ralstonia sp.
G4XDR8
-
-
1.20.1.1
Ralstonia sp. 4506
G4XDR8
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.1.1
phosphonate + NAD+ + H2O
-
728004
Pseudomonas stutzeri
phosphate + NADH + H+
-
-
-
?
1.20.1.1
phosphonate + NAD+ + H2O
-
728004
Ralstonia sp.
phosphate + NADH + H+
-
-
-
?
1.20.1.1
phosphonate + NAD+ + H2O
-
728004
Pseudomonas stutzeri WM88
phosphate + NADH + H+
-
-
-
?
1.20.1.1
phosphonate + NAD+ + H2O
-
728004
Ralstonia sp. 4506
phosphate + NADH + H+
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.20.1.1
50
-
-
Pseudomonas stutzeri
1.20.1.1
50
-
-
Ralstonia sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.20.1.1
45
-
half-life 80.5 h
Pseudomonas stutzeri
1.20.1.1
45
-
half-life 80.5 h
Ralstonia sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.20.1.1
expression in Escherichia coli
Pseudomonas stutzeri
1.20.1.1
expression in Escherichia coli
Ralstonia sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.20.1.1
arsenite
4 mM, 90% residual activity
Pseudomonas stutzeri
1.20.1.1
additional information
not inhibitory: arsenite, NaCl
Ralstonia sp.
1.20.1.1
NaCl
4 mM, 87% residual activity
Pseudomonas stutzeri
1.20.1.1
nitrate
4 mM, 21% residual activity
Pseudomonas stutzeri
1.20.1.1
nitrate
4 mM, 40% residual activity
Ralstonia sp.
1.20.1.1
sulfite
4 mM, 16% residual activity
Pseudomonas stutzeri
1.20.1.1
sulfite
4 mM, 56% residual activity
Ralstonia sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.1.1
0.0026
-
NAD+
pH 7.2, 30°C
Ralstonia sp.
1.20.1.1
0.0073
-
phosphonate
pH 7.2, 30°C
Pseudomonas stutzeri
1.20.1.1
0.0087
-
phosphonate
pH 7.2, 30°C
Ralstonia sp.
1.20.1.1
0.016
-
NAD+
pH 7.2, 40°C
Ralstonia sp.
1.20.1.1
0.024
-
phosphonate
pH 7.2, 40°C
Ralstonia sp.
1.20.1.1
0.056
-
NAD+
pH 7.2, 30°C
Pseudomonas stutzeri
1.20.1.1
0.128
-
NAD+
pH 7.2, 40°C
Pseudomonas stutzeri
1.20.1.1
0.287
-
phosphonate
pH 7.2, 40°C
Pseudomonas stutzeri
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.20.1.1
soluble
recombinant protein
Ralstonia sp.
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.1.1
phosphonate + NAD+ + H2O
-
728004
Pseudomonas stutzeri
phosphate + NADH + H+
-
-
-
?
1.20.1.1
phosphonate + NAD+ + H2O
-
728004
Ralstonia sp.
phosphate + NADH + H+
-
-
-
?
1.20.1.1
phosphonate + NAD+ + H2O
-
728004
Pseudomonas stutzeri WM88
phosphate + NADH + H+
-
-
-
?
1.20.1.1
phosphonate + NAD+ + H2O
-
728004
Ralstonia sp. 4506
phosphate + NADH + H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.20.1.1
50
-
-
Pseudomonas stutzeri
1.20.1.1
50
-
-
Ralstonia sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.20.1.1
45
-
half-life 80.5 h
Pseudomonas stutzeri
1.20.1.1
45
-
half-life 80.5 h
Ralstonia sp.