EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.98 | expressed in Escherichia coli ubiX and ubiD deletion mutant | Synechocystis sp. |
4.1.3.40 | gene sll1797, phylogenetic analysis and tree, expression in Escherichia coli strain BL21AI as GST-tagged protein, and in Escherichia coli strain BW25113 for quantitative expression analysis by real time quantitative PCR. Complementation of ubiquinone-deficient Escherichia coli mutants by catalytic activity of heterologous Sll1797 | Synechocystis sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.3.40 | additional information | generation of a Synechocystis sp. single-gene knockout mutant lacking the chorismate pyruvate-lyase Sll1797 | Synechocystis sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.1.98 | membrane | - |
Synechocystis sp. | 16020 | - |
4.1.3.40 | membrane | the recombinant enzyme shows severalfold higher specific activities in membrane than in soluble protein fractions | Synechocystis sp. | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.98 | additional information | the addition of divalent cations does not stimulate the enzyme activity | Synechocystis sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.98 | a 4-hydroxy-3-polyprenylbenzoate | Synechocystis sp. | - |
a 2-polyprenylphenol + CO2 | - |
? | |
4.1.3.40 | chorismate | Synechocystis sp. | - |
4-hydroxybenzoate + pyruvate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.98 | Synechocystis sp. | - |
- |
- |
4.1.3.40 | Synechocystis sp. | P73322 | gene sll1797 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.40 | recombinant GST-tagged enzyme from Escherichia coli strain BL21AI by glutathione affinity chromatography | Synechocystis sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.98 | 4-hydroxy-3-farnesylbenzoate | highest activity with 4-hydroxy-3-farnesylbenzoate | Synechocystis sp. | ? + CO2 | - |
? | |
4.1.1.98 | 4-hydroxy-3-geranylgeranylbenzoate | - |
Synechocystis sp. | ? + CO2 | - |
? | |
4.1.1.98 | a 4-hydroxy-3-polyprenylbenzoate | - |
Synechocystis sp. | a 2-polyprenylphenol + CO2 | - |
? | |
4.1.1.98 | additional information | very low specific activity with 4-hydroxy-3-octaprenylbenzoate | Synechocystis sp. | ? | - |
? | |
4.1.3.40 | chorismate | - |
Synechocystis sp. | 4-hydroxybenzoate + pyruvate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.98 | 4-hydroxy-3-solanesylbenzoate decarboxylase | - |
Synechocystis sp. |
4.1.1.98 | slr1099 | - |
Synechocystis sp. |
4.1.3.40 | chorismate pyruvate-lyase | - |
Synechocystis sp. |
4.1.3.40 | Sll1797 | - |
Synechocystis sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.98 | 30 | - |
- |
Synechocystis sp. |
4.1.3.40 | 30 | - |
- |
Synechocystis sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.98 | 8.5 | - |
- |
Synechocystis sp. |
4.1.3.40 | 8 | - |
- |
Synechocystis sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.1.98 | malfunction | disruption of the slr1099 gene results in reduced growth rates and plastoquinone contents | Synechocystis sp. |
4.1.3.40 | malfunction | a knockout mutant shows highyl reduced plastoquinone content, and the mutant requires 4-hydroxybenzoate for growth | Synechocystis sp. |
4.1.3.40 | metabolism | the enzyme is required for initiation of cyanobacterial plastoquinone biosynthesis, together with two 4-hydroxy-3-solanesylbenzoate decarboxylases. The chorismate pyruvate-lyase Sll1797 catalyzes the conversion of chorismate to 4-hydroxybenzoate that is prenylated by Slr0926. Slr1099 and Sll0936 are both involved in the decarboxylation of 4-hydroxy-3-solanesylbenzoate | Synechocystis sp. |