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Literature summary extracted from

  • Dela Sena, C.; Narayanasamy, S.; Riedl, K.M.; Curley, R.W.; Schwartz, S.J.; Harrison, E.H.
    Substrate specificity of purified recombinant human beta-carotene 15,15'-oxygenase (BCO1) (2013), J. Biol. Chem., 288, 37094-37103.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.63 expression of BCO1 with a C-terminal polyhistidine tag in Escherichia coli strain BL21-Gold(DE3) Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.63 0.0172
-
 beta-carotene recombinant His6-tagged enzyme, pH and temperature not specified in the publication Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.63 63702
-
 x * 63702, His-tagged enzyme, sequence calculation Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.63 beta-carotene + O2 Homo sapiens
-
 2 all-trans-retinal
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.63 Homo sapiens Q9HAY6 gene BCMO1; gene Bcmo1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.63 recombinant BCO1 with a C-terminal polyhistidine tag from Escherichia coli strain BL21-Gold(DE3) by cobalt affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.63 0.0022
-
 purified recombinant His6-tagged enzyme, pH and temperature not specified in the publication Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.63 alpha-carotene + O2
-
 Homo sapiens 2 retinal
-
 ?
1.13.11.63 beta-apo-8'-carotenal + O2
-
 Homo sapiens retinal + ?
-
 ?
1.13.11.63 beta-carotene + O2
-
 Homo sapiens 2 all-trans-retinal
-
 ?
1.13.11.63 beta-cryptoxanthin + O2
-
 Homo sapiens 2 retinal
-
 ?
1.13.11.63 lycopene + O2
-
 Homo sapiens 2 acycloretinal
-
 ?
1.13.11.63 additional information shorter beta-apocarotenals, i.e. beta-apo-10-carotenal, beta-apo-12'-carotenal, and beta-apo-14'-carotenal, do not show Michaelis-Menten behavior under the conditions tested. No activity with lutein, zeaxanthin, and 9-cis-beta-carotene. Purified recombinant BCO1 cleaves beta-carotene solely at the central 15-15' bond Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.63 ? x * 63702, His-tagged enzyme, sequence calculation Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.13.11.63 BCO1
-
 Homo sapiens
1.13.11.63 beta-carotene 15,15'-oxygenase
-
 Homo sapiens