EC Number | Application | Comment | Organism |
---|---|---|---|
1.13.11.63 | synthesis | stepwise cleavage by BCO2, i.e. beta-carotene-9',10'-oxygenase, and BCO1 with beta-apo-10'-carotenol as an intermediate can provide a mechanism to tailor asymmetric carotenoids such as beta-cryptoxanthin for vitamin A production | Mus musculus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.71 | expressed in Escherichia coli | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.11.63 | additional information | subjection of wild type, Bco1-/-, Bco2-/-, and Bco1-/-/Bco2-/- double knock-out mice to a controlled diet providing beta-carotene as the sole source for apocarotenoid production | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.63 | beta-apo-10'-carotenol + O2 | Mus musculus | beta-apo-10'-carotenol is the major long-chain beta-apocarotenoid in mouse liver | ? | - |
? | |
1.13.11.63 | beta-carotene + O2 | Mus musculus | - |
2 all-trans-retinal | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.63 | Mus musculus | Q9JJS6 | - |
- |
1.13.11.71 | Mus musculus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.63 | liver | - |
Mus musculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.63 | beta-apo-10'-carotenol + O2 | beta-apo-10'-carotenol is the major long-chain beta-apocarotenoid in mouse liver | Mus musculus | ? | - |
? | |
1.13.11.63 | beta-carotene + O2 | - |
Mus musculus | 2 all-trans-retinal | - |
? | |
1.13.11.63 | additional information | symmetric cleavage by BCO1 yields retinoids, i.e. beta-15'-apocarotenoids, C20 | Mus musculus | ? | - |
? | |
1.13.11.71 | beta-cryptoxanthin + O2 | main clevage product is beta-apo-10'-carotenal only small amounts of 3-OH-beta-apocarotenal are formed | Mus musculus | beta-apo-10'-carotenal + ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.63 | BCO1 | - |
Mus musculus |
1.13.11.63 | beta-carotene-15,15'-oxygenase | - |
Mus musculus |
1.13.11.71 | BCO2 | - |
Mus musculus |
1.13.11.71 | beta-carotene-9',10'-oxygenase | - |
Mus musculus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.71 | 28 | - |
assay at | Mus musculus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.11.71 | malfunction | using BCO2 knock out mice it is shown that BCO2 catalyzes beta-apocarotenoid production in vivo. Bco2-/- mice show a significant hepatic accumulation of beta-cryptoxanthin as compared to Bco1-/- and wild-type mice | Mus musculus |
1.14.99.36 | malfunction | genetic disruption of BCO1 results in beta-carotene accumulation and vitamin A deficiency accompanied by a BCO2-dependent production of minor amounts of beta-apo-10'-carotenol, which can be esterified and transported by the same proteins as vitamin A but with a lower affinity and slower reaction kinetics. all-trans-Retinol treatment of vitamin A-deprived Bco1-/- mice decreases hepatic retinol-binding protein levels | Mus musculus |
1.14.99.36 | physiological function | beta-carotene-15,15'-oxygenase BCO1, but not beta-carotene-9',10'-oxygenase BCO2, is critical for retinoid homeostasis. In wild-type mice, beta-apo-10'-carotenol is converted to retinoids by BCO1 | Mus musculus |