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Literature summary extracted from

  • Hess, V.; Schuchmann, K.; Müller, V.
    The ferredoxin: NAD+ oxidoreductase (Rnf) from the acetogen Acetobacterium woodii requires Na+ and is reversibly coupled to the membrane potential (2013), J. Biol. Chem., 288, 31496-31502.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.2.1.2 ETH2120 reduced ferredoxin:NAD+ oxidoreductase activity at inverted membrane vesicles is not stimulated to 155% of control Acetobacterium woodii
7.2.1.2 monensin reduced ferredoxin:NAD+ oxidoreductase activity at inverted membrane vesicles is not stimulated to 138% of control Acetobacterium woodii
7.2.1.2 valinomycin reduced ferredoxin:NAD+ oxidoreductase activity at inverted membrane vesicles is not stimulated to 128% of control Acetobacterium woodii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.2.1.2 0.085
-
NAD+ pH 7.7, 30°C Acetobacterium woodii
7.2.1.2 0.155
-
Na+/in pH 6, 30°C Acetobacterium woodii
7.2.1.2 0.201
-
Na+/in pH 7.7, 30°C Acetobacterium woodii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.3 membrane
-
Acetobacterium woodii 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.3 reduced ferredoxin + NAD+ Acetobacterium woodii ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles oxidized ferredoxin + NADH + H+
-
r
1.18.1.3 reduced ferredoxin + NAD+ Acetobacterium woodii DSM 103 ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles oxidized ferredoxin + NADH + H+
-
r
1.18.1.3 reduced ferredoxin + NAD+ Acetobacterium woodii DSM 1030 ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles oxidized ferredoxin + NADH + H+
-
r
7.2.1.2 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in Acetobacterium woodii electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
7.2.1.2 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in Acetobacterium woodii DSM 103 electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
7.2.1.2 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in Acetobacterium woodii DSM 1030 electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.3 Acetobacterium woodii
-
-
-
1.18.1.3 Acetobacterium woodii DSM 103
-
-
-
7.2.1.2 Acetobacterium woodii
-
-
-
7.2.1.2 Acetobacterium woodii DSM 1030
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.3 50000
-
substrate CO-reduced ferredoxin, pH 7.7, 30°C Acetobacterium woodii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.3 oxidized ferredoxin + NADH + H+ endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a membrane potential, and is accompanied by Na+ efflux out of the vesicles Acetobacterium woodii reduced ferredoxin + NAD+
-
r
1.18.1.3 oxidized ferredoxin + NADH + H+ endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a membrane potential, and is accompanied by Na+ efflux out of the vesicles Acetobacterium woodii DSM 103 reduced ferredoxin + NAD+
-
r
1.18.1.3 oxidized ferredoxin + NADH + H+ endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a membrane potential, and is accompanied by Na+ efflux out of the vesicles Acetobacterium woodii DSM 1030 reduced ferredoxin + NAD+
-
r
1.18.1.3 reduced ferredoxin + NAD+ ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles Acetobacterium woodii oxidized ferredoxin + NADH + H+
-
r
1.18.1.3 reduced ferredoxin + NAD+ ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles Acetobacterium woodii DSM 103 oxidized ferredoxin + NADH + H+
-
r
1.18.1.3 reduced ferredoxin + NAD+ ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles Acetobacterium woodii DSM 1030 oxidized ferredoxin + NADH + H+
-
r
7.2.1.2 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport Acetobacterium woodii 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
7.2.1.2 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport. Low activity in absence of Na+ Acetobacterium woodii 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
7.2.1.2 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport Acetobacterium woodii DSM 103 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
7.2.1.2 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport. Low activity in absence of Na+ Acetobacterium woodii DSM 103 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
7.2.1.2 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport Acetobacterium woodii DSM 1030 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?
7.2.1.2 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport. Low activity in absence of Na+ Acetobacterium woodii DSM 1030 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
?

Synonyms

EC Number Synonyms Comment Organism
7.2.1.2 reduced ferredoxin:NAD+ oxidoreductase
-
Acetobacterium woodii
7.2.1.2 Rnf complex
-
Acetobacterium woodii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.2.1.2 30
-
assay at Acetobacterium woodii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2.1.2 7 8
-
Acetobacterium woodii
7.2.1.2 7.7
-
assay at Acetobacterium woodii

General Information

EC Number General Information Comment Organism
7.2.1.2 metabolism electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport Acetobacterium woodii