EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
7.2.1.2 | ETH2120 | reduced ferredoxin:NAD+ oxidoreductase activity at inverted membrane vesicles is not stimulated to 155% of control | Acetobacterium woodii | |
7.2.1.2 | monensin | reduced ferredoxin:NAD+ oxidoreductase activity at inverted membrane vesicles is not stimulated to 138% of control | Acetobacterium woodii | |
7.2.1.2 | valinomycin | reduced ferredoxin:NAD+ oxidoreductase activity at inverted membrane vesicles is not stimulated to 128% of control | Acetobacterium woodii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.2.1.2 | 0.085 | - |
NAD+ | pH 7.7, 30°C | Acetobacterium woodii | |
7.2.1.2 | 0.155 | - |
Na+/in | pH 6, 30°C | Acetobacterium woodii | |
7.2.1.2 | 0.201 | - |
Na+/in | pH 7.7, 30°C | Acetobacterium woodii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.18.1.3 | membrane | - |
Acetobacterium woodii | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.3 | reduced ferredoxin + NAD+ | Acetobacterium woodii | ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles | oxidized ferredoxin + NADH + H+ | - |
r | |
1.18.1.3 | reduced ferredoxin + NAD+ | Acetobacterium woodii DSM 103 | ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles | oxidized ferredoxin + NADH + H+ | - |
r | |
1.18.1.3 | reduced ferredoxin + NAD+ | Acetobacterium woodii DSM 1030 | ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles | oxidized ferredoxin + NADH + H+ | - |
r | |
7.2.1.2 | 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in | Acetobacterium woodii | electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport | 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out | - |
? | |
7.2.1.2 | 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in | Acetobacterium woodii DSM 103 | electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport | 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out | - |
? | |
7.2.1.2 | 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in | Acetobacterium woodii DSM 1030 | electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport | 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.1.3 | Acetobacterium woodii | - |
- |
- |
1.18.1.3 | Acetobacterium woodii DSM 103 | - |
- |
- |
7.2.1.2 | Acetobacterium woodii | - |
- |
- |
7.2.1.2 | Acetobacterium woodii DSM 1030 | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.18.1.3 | 50000 | - |
substrate CO-reduced ferredoxin, pH 7.7, 30°C | Acetobacterium woodii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.3 | oxidized ferredoxin + NADH + H+ | endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a membrane potential, and is accompanied by Na+ efflux out of the vesicles | Acetobacterium woodii | reduced ferredoxin + NAD+ | - |
r | |
1.18.1.3 | oxidized ferredoxin + NADH + H+ | endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a membrane potential, and is accompanied by Na+ efflux out of the vesicles | Acetobacterium woodii DSM 103 | reduced ferredoxin + NAD+ | - |
r | |
1.18.1.3 | oxidized ferredoxin + NADH + H+ | endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a membrane potential, and is accompanied by Na+ efflux out of the vesicles | Acetobacterium woodii DSM 1030 | reduced ferredoxin + NAD+ | - |
r | |
1.18.1.3 | reduced ferredoxin + NAD+ | ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles | Acetobacterium woodii | oxidized ferredoxin + NADH + H+ | - |
r | |
1.18.1.3 | reduced ferredoxin + NAD+ | ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles | Acetobacterium woodii DSM 103 | oxidized ferredoxin + NADH + H+ | - |
r | |
1.18.1.3 | reduced ferredoxin + NAD+ | ferredoxin is reduced with CO, catalyzed by the purified acetyl-CoA synthase/CO dehydrogenase. Using CO-reduced ferredoxin, NAD+ reduction is highly specific and strictly dependent on ferredoxin. Electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport, indicating the generation of a sodium ion potential. Vice versa, endergonic ferredoxin reduction with NADH as reductant is possible, but only in the presence of a sodium ion potential, and is accompanied by Na+ efflux out of the vesicles | Acetobacterium woodii DSM 1030 | oxidized ferredoxin + NADH + H+ | - |
r | |
7.2.1.2 | 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in | electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport | Acetobacterium woodii | 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out | - |
? | |
7.2.1.2 | 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in | electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport. Low activity in absence of Na+ | Acetobacterium woodii | 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out | - |
? | |
7.2.1.2 | 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in | electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport | Acetobacterium woodii DSM 103 | 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out | - |
? | |
7.2.1.2 | 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in | electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport. Low activity in absence of Na+ | Acetobacterium woodii DSM 103 | 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out | - |
? | |
7.2.1.2 | 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in | electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport | Acetobacterium woodii DSM 1030 | 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out | - |
? | |
7.2.1.2 | 2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in | electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport. Low activity in absence of Na+ | Acetobacterium woodii DSM 1030 | 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.2.1.2 | reduced ferredoxin:NAD+ oxidoreductase | - |
Acetobacterium woodii |
7.2.1.2 | Rnf complex | - |
Acetobacterium woodii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
7.2.1.2 | 30 | - |
assay at | Acetobacterium woodii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.2.1.2 | 7 | 8 | - |
Acetobacterium woodii |
7.2.1.2 | 7.7 | - |
assay at | Acetobacterium woodii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
7.2.1.2 | metabolism | electron transport from reduced ferredoxin to NAD+ is coupled to electrogenic Na+ transport | Acetobacterium woodii |