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Literature summary extracted from

  • Grishin, A.M.; Ajamian, E.; Zhang, L.; Rouiller, I.; Bostina, M.; Cygler, M.
    Protein-protein interactions in the beta-oxidation part of the phenylacetate utilization pathway: crystal structure of the PaaF-PaaG hydratase-isomerase complex (2012), J. Biol. Chem., 287, 37986-37996.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.3.18 sitting drop vapor diffusion method, using 0.1 M sodium citrate, pH 5.5, 0.2 M sodium acetate, and 5% (w/w) PEG 4000 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.18 28779
-
 3 * 28779, MALDI-TOF mass spectrometry Thermus thermophilus
5.3.3.18 30000
-
 3 * 30000, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.18 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA Thermus thermophilus
-
 2-oxepin-2(3H)-ylideneacetyl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.18 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.18 Ni-NTA beads chromatography and Superdex 200 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.18 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
-
 Thermus thermophilus 2-oxepin-2(3H)-ylideneacetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.3.18 homotrimer 3 * 30000, SDS-PAGE Thermus thermophilus
5.3.3.18 homotrimer 3 * 28779, MALDI-TOF mass spectrometry Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
5.3.3.18 paaG
-
 Thermus thermophilus