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Literature summary extracted from

  • Montgomery, C.; Pei, Z.; Watkins, P.A.; Miziorko, H.M.
    Identification and characterization of an extramitochondrial human 3-hydroxy-3-methylglutaryl-CoA lyase (2012), J. Biol. Chem., 287, 33227-33236.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.4 cloning of 3-hydroxy-3-methylglutaryl-CoA lyase-like protein, sequence comparison of HMGCLL1 and HMGCL, expression of 3-hydroxy-3-methylglutaryl-CoA lyase-like protein mutant G2A in COS-1 cell cytosol, recombinant expression of wild-type 3-hydroxy-3-methylglutaryl-CoA lyase-like protein in Escherichia coli and Pichia pastoris, in insoluble form Homo sapiens
4.1.3.4 sequence comparison of HMGCLL1 and HMGCL, expression of 3-hydroxy-3-methylglutaryl-CoA lyase wild-type in COS-1 cell cytosol, recombinant expression of wild-type 3-hydroxy-3-methylglutaryl-CoA lyase in Escherichia coli and Pichia pastoris, in active form Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.4 C323S site-directed mutagenesis of HMGCL, the mutant shows 2fold increased activity compared to wild-type HMGCL Homo sapiens
4.1.3.4 G2A site-directed mutagenesis of HMGCLL1, eliminatin of N-terminal myristoylation motif by construction of a 3-hydroxy-3-methylglutaryl-CoA lyase-like protein HMGCLL1 G2A mutant, the mutant shows reduced activity compared to wild-type HMGCLL1 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.4 0.024
-
 (S)-3-hydroxy-3-methylglutaryl-CoA HMGCLL1 mutant G2A and wild-type HMGCL, pH 8.2, 37°C Homo sapiens
4.1.3.4 0.024
-
 (S)-3-hydroxy-3-methylglutaryl-CoA wild-type HMGCL, pH 8.2, 37°C Homo sapiens
4.1.3.4 0.028
-
 (S)-3-hydroxy-3-methylglutaryl-CoA wild-type HMGCLL1, pH 8.2, 37°C Homo sapiens
4.1.3.4 0.045
-
 (S)-3-hydroxy-3-methylglutaryl-CoA wild-type HMGCLL1, pH 8.2, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.3.4 Golgi-derived vesicle endogenous HMGCLL1 localized to punctate vesicles Homo sapiens
-
-
4.1.3.4 mitochondrion 3-hydroxy-3-methylglutaryl-CoA lyase Homo sapiens 5739
-
4.1.3.4 additional information HMGCL is a mitochondrial enzyme Homo sapiens
-
-
4.1.3.4 additional information HMGCLL1 is an extramitochondrial enzyme Homo sapiens
-
-
4.1.3.4 perinuclear space wild-type 3-hydroxy-3-methylglutaryl-CoA lyase-like protein, myristoylation dependent association with nonmitochondrial membrane compartments Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.4 Mg2+ required, Km for wild-type HMGCL is 0.233 mM and for HMGCL mutant C323A 0.322 mM Homo sapiens
4.1.3.4 Mg2+ required, Km of wild-type HMGCLL1 is 0.049 mM and for HMGCLL1 mutant G2A 0.088 mM Homo sapiens
4.1.3.4 Mn2+ required, Km for wild-type HMGCL is 0.00034 mM and for HMGCL mutant C323A 0.00037 mM Homo sapiens
4.1.3.4 Mn2+ required, Km of wild-type HMGCLL1 is 0.00018 mM and for HMGCLL1 mutant G2A 0.00024 mM Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.4 (S)-3-Hydroxy-3-methylglutaryl-CoA Homo sapiens
-
 Acetyl-CoA + acetoacetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.4 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.3.4 lipoprotein the deduced 3-hydroxy-3-methylglutaryl-CoA lyase-like protein, HMGCLL1, sequence contains an N-terminal myristoylation motif. Myristoylation of HMGCLL1 affects its cellular localization Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.4 recombinant active wild-type 3-hydroxy-3-methylglutaryl-CoA lyase-like protein and its mutant G2A from Pichia pastoris by nickel affinity chromatography and ultrafiltration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.4 adrenal gland HMGCL Homo sapiens
-
4.1.3.4 brain cancer cell HMGCLL1 is overexpressed in certain types of human brain cancer cells Homo sapiens
-
4.1.3.4 cecum HMGCL Homo sapiens
-
4.1.3.4 duodenum HMGCL Homo sapiens
-
4.1.3.4 duodenum HMGCLL1 Homo sapiens
-
4.1.3.4 glioblastoma cell HMGCLL1 Homo sapiens
-
4.1.3.4 kidney HMGCL Homo sapiens
-
4.1.3.4 large intestine HMGCL Homo sapiens
-
4.1.3.4 liver HMGCL Homo sapiens
-
4.1.3.4 additional information tissue distribution of 3-hydroxy-3-methylglutaryl-CoA lyase, overview Homo sapiens
-
4.1.3.4 additional information tissue distribution of 3-hydroxy-3-methylglutaryl-CoA lyase-like protein, overview Homo sapiens
-
4.1.3.4 small intestine HMGCL Homo sapiens
-
4.1.3.4 small intestine HMGCLL1 Homo sapiens
-
4.1.3.4 U-87MG cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.4 117
-
 HMGCLL1 mutant G2A, pH 8.2, 37°C Homo sapiens
4.1.3.4 150
-
 wild-type HMGCLL1, pH 8.2, 37°C Homo sapiens
4.1.3.4 159
-
 wild-type HMGCL, pH 8.2, 37°C Homo sapiens
4.1.3.4 348
-
 HMGCL mutant C323A, pH 8.2, 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.4 (S)-3-Hydroxy-3-methylglutaryl-CoA
-
 Homo sapiens Acetyl-CoA + acetoacetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.4 3-Hydroxy-3-methylglutaryl-CoA lyase
-
 Homo sapiens
4.1.3.4 3-hydroxy-3-methylglutaryl-CoA lyase-like protein
-
 Homo sapiens
4.1.3.4 HMG-CoA lyase
-
 Homo sapiens
4.1.3.4 HMGCL
-
 Homo sapiens
4.1.3.4 HMGCLL1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.4 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.4 8.2
-
 assay at Homo sapiens