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Literature summary extracted from

  • Kumar, S.; Das, M.; Hadad, C.M.; Musier-Forsyth, K.
    Substrate specificity of bacterial prolyl-tRNA synthetase editing domain is controlled by a tunable hydrophobic pocket (2012), J. Biol. Chem., 287, 3175-3184.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.15 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.15 H366A the mutant shows loss in L-alanine deacylation activity Escherichia coli
6.1.1.15 L266A the mutant shows negligible in L-alanine deacylation at room temperature Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro Escherichia coli
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.15 Talon cobalt affinity resin column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-alanine + tRNAPro
-
 Escherichia coli AMP + diphosphate + L-alanyl-tRNAPro
-
 r
6.1.1.15 ATP + L-cysteine + tRNAPro
-
 Escherichia coli AMP + diphosphate + L-cysteinyl-tRNAPro
-
 r
6.1.1.15 ATP + L-proline + tRNAPro
-
 Escherichia coli AMP + diphosphate + L-prolyl-tRNAPro
-
 r

Synonyms

EC Number Synonyms Comment Organism
6.1.1.15 Prolyl-tRNA synthetase
-
 Escherichia coli
6.1.1.15 ProRS
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.15 ATP
-
 Escherichia coli