Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Thotsaporn, K.; Chenprakhon, P.; Sucharitakul, J.; Mattevi, A.; Chaiyen, P.
    Stabilization of C4a-hydroperoxyflavin in a two-component flavin-dependent monooxygenase is achieved through interactions at flavin N5 and C4a atoms (2011), J. Biol. Chem., 286, 28170-28180.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.9 expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.9 S171A the mutant shows reduced activity compared to the wild type enzyme Thermus thermophilus
1.14.14.9 S171A/H396V inactive Thermus thermophilus
1.14.14.9 S171T the mutant shows about wild type activity Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.9 4-hydroxyphenylacetate + FMNH + O2 Thermus thermophilus
-
 3,4-dihydroxyphenylacetate + FMN + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + FMNH + O2 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 3,4-dihydroxyphenylacetate + FMN + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.9 Thermus thermophilus
-
-
-
1.14.14.9 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.9 4-hydroxyphenylacetate + FMNH + O2
-
 Thermus thermophilus 3,4-dihydroxyphenylacetate + FMN + H2O
-
 ?
1.14.14.9 4-hydroxyphenylacetate + FMNH + O2
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 3,4-dihydroxyphenylacetate + FMN + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.9 HPAH
-
 Thermus thermophilus
1.14.14.9 p-hydroxyphenylacetate 3-hydroxylase
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.9 flavin dependent on Thermus thermophilus