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Literature summary extracted from

  • Steen, I.H.; Madern, D.; Karlström, M.; Lien, T.; Ladenstein, R.; Birkeland, N.K.
    Comparison of isocitrate dehydrogenase from three hyperthermophiles reveals differences in thermostability, cofactor specificity, oligomeric state, and phylogenetic affiliation (2001), J. Biol. Chem., 276, 43924-43931.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.41 0.0683
-
NAD+ pH 8.0, 70°C, the Km-value for NADP+ is about 65fold higher than the KM-value for NAD+ Pyrococcus furiosus
1.1.1.42 0.0444
-
NADP+ pH 8.0, 70°C Aeropyrum pernix
1.1.1.42 0.0552
-
NADP+ pH 8.0, 70°C, Km-value for NAD+ is about 140fold higher than the KM-value for NADP+ Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.41 44300
-
2 * 44300, calculated from sequence Pyrococcus furiosus
1.1.1.41 70000
-
analytical ultracentrifugation Pyrococcus furiosus
1.1.1.42 45400
-
2 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence Thermotoga maritima
1.1.1.42 45400
-
4 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence Thermotoga maritima
1.1.1.42 47900
-
2 * 47900, calculated from sequence Aeropyrum pernix
1.1.1.42 68000
-
dimer, analytical ultracentrifugation Thermotoga maritima
1.1.1.42 70000
-
analytical ultracentrifugation Aeropyrum pernix
1.1.1.42 174000
-
tetramer, analytical ultracentrifugation Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Thermotoga maritima
-
2-oxoglutarate + CO2 + NADPH + H+
-
?
1.1.1.42 isocitrate + NADP+ Aeropyrum pernix
-
2-oxoglutarate + CO2 + NADPH + H+
-
?
1.1.1.42 isocitrate + NADP+ Aeropyrum pernix DSM 11879
-
2-oxoglutarate + CO2 + NADPH + H+
-
?
1.1.1.42 isocitrate + NADP+ Thermotoga maritima DSM 3109
-
2-oxoglutarate + CO2 + NADPH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Pyrococcus furiosus Q8U488
-
-
1.1.1.42 Aeropyrum pernix Q9YE81
-
-
1.1.1.42 Aeropyrum pernix DSM 11879 Q9YE81
-
-
1.1.1.42 Thermotoga maritima Q9X0N2
-
-
1.1.1.42 Thermotoga maritima DSM 3109 Q9X0N2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41
-
Pyrococcus furiosus
1.1.1.42
-
Thermotoga maritima
1.1.1.42
-
Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 isocitrate + NAD+ low activity with NADP+. Km-value for NADP+ is about 65fold higher than the KM-value for NAD+ Pyrococcus furiosus 2-oxoglutarate + CO2 + NADH + H+
-
?
1.1.1.42 isocitrate + NADP+
-
Thermotoga maritima 2-oxoglutarate + CO2 + NADPH + H+
-
?
1.1.1.42 isocitrate + NADP+
-
Aeropyrum pernix 2-oxoglutarate + CO2 + NADPH + H+
-
?
1.1.1.42 isocitrate + NADP+ low activity with NAD+. Km-value for NAD+ is about 140fold higher than the KM-value for NADP+ Thermotoga maritima 2-oxoglutarate + CO2 + NADPH + H+
-
?
1.1.1.42 isocitrate + NADP+
-
Aeropyrum pernix DSM 11879 2-oxoglutarate + CO2 + NADPH + H+
-
?
1.1.1.42 isocitrate + NADP+
-
Thermotoga maritima DSM 3109 2-oxoglutarate + CO2 + NADPH + H+
-
?
1.1.1.42 isocitrate + NADP+ low activity with NAD+. Km-value for NAD+ is about 140fold higher than the KM-value for NADP+ Thermotoga maritima DSM 3109 2-oxoglutarate + CO2 + NADPH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.41 homodimer 2 * 44300, calculated from sequence Pyrococcus furiosus
1.1.1.42 dimer 2 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence Thermotoga maritima
1.1.1.42 homodimer 2 * 47900, calculated from sequence Aeropyrum pernix
1.1.1.42 tetramer 4 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 NAD-IDH
-
Pyrococcus furiosus
1.1.1.41 PF0202
-
Pyrococcus furiosus
1.1.1.42 NADP-IDH
-
Thermotoga maritima
1.1.1.42 NADP-IDH
-
Aeropyrum pernix
1.1.1.42 TM1148
-
Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.41 95
-
above Pyrococcus furiosus
1.1.1.42 90
-
-
Thermotoga maritima
1.1.1.42 95
-
above Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.41 additional information
-
thermal denaturation is an irreversible process Pyrococcus furiosus
1.1.1.41 104
-
the Tm-value is 103.7°C Pyrococcus furiosus
1.1.1.42 additional information
-
thermal denaturation is an irreversible process Thermotoga maritima
1.1.1.42 additional information
-
thermal denaturation is an irreversible process Aeropyrum pernix
1.1.1.42 98
-
the Tm-value is 98.3°C Thermotoga maritima
1.1.1.42 110
-
the Tm-value is 109.9°C Aeropyrum pernix