EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.41 | 0.0683 | - |
NAD+ | pH 8.0, 70°C, the Km-value for NADP+ is about 65fold higher than the KM-value for NAD+ | Pyrococcus furiosus | |
1.1.1.42 | 0.0444 | - |
NADP+ | pH 8.0, 70°C | Aeropyrum pernix | |
1.1.1.42 | 0.0552 | - |
NADP+ | pH 8.0, 70°C, Km-value for NAD+ is about 140fold higher than the KM-value for NADP+ | Thermotoga maritima |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 44300 | - |
2 * 44300, calculated from sequence | Pyrococcus furiosus |
1.1.1.41 | 70000 | - |
analytical ultracentrifugation | Pyrococcus furiosus |
1.1.1.42 | 45400 | - |
2 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence | Thermotoga maritima |
1.1.1.42 | 45400 | - |
4 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence | Thermotoga maritima |
1.1.1.42 | 47900 | - |
2 * 47900, calculated from sequence | Aeropyrum pernix |
1.1.1.42 | 68000 | - |
dimer, analytical ultracentrifugation | Thermotoga maritima |
1.1.1.42 | 70000 | - |
analytical ultracentrifugation | Aeropyrum pernix |
1.1.1.42 | 174000 | - |
tetramer, analytical ultracentrifugation | Thermotoga maritima |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.42 | isocitrate + NADP+ | Thermotoga maritima | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | Aeropyrum pernix | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | Aeropyrum pernix DSM 11879 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | Thermotoga maritima DSM 3109 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.41 | Pyrococcus furiosus | Q8U488 | - |
- |
1.1.1.42 | Aeropyrum pernix | Q9YE81 | - |
- |
1.1.1.42 | Aeropyrum pernix DSM 11879 | Q9YE81 | - |
- |
1.1.1.42 | Thermotoga maritima | Q9X0N2 | - |
- |
1.1.1.42 | Thermotoga maritima DSM 3109 | Q9X0N2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.41 | - |
Pyrococcus furiosus |
1.1.1.42 | - |
Thermotoga maritima |
1.1.1.42 | - |
Aeropyrum pernix |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.41 | isocitrate + NAD+ | low activity with NADP+. Km-value for NADP+ is about 65fold higher than the KM-value for NAD+ | Pyrococcus furiosus | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | - |
Thermotoga maritima | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | - |
Aeropyrum pernix | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | low activity with NAD+. Km-value for NAD+ is about 140fold higher than the KM-value for NADP+ | Thermotoga maritima | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | - |
Aeropyrum pernix DSM 11879 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | - |
Thermotoga maritima DSM 3109 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | low activity with NAD+. Km-value for NAD+ is about 140fold higher than the KM-value for NADP+ | Thermotoga maritima DSM 3109 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.41 | homodimer | 2 * 44300, calculated from sequence | Pyrococcus furiosus |
1.1.1.42 | dimer | 2 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence | Thermotoga maritima |
1.1.1.42 | homodimer | 2 * 47900, calculated from sequence | Aeropyrum pernix |
1.1.1.42 | tetramer | 4 * 45400, equilibrium of dimeric and tetrameric species, calculated from sequence | Thermotoga maritima |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.41 | NAD-IDH | - |
Pyrococcus furiosus |
1.1.1.41 | PF0202 | - |
Pyrococcus furiosus |
1.1.1.42 | NADP-IDH | - |
Thermotoga maritima |
1.1.1.42 | NADP-IDH | - |
Aeropyrum pernix |
1.1.1.42 | TM1148 | - |
Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 95 | - |
above | Pyrococcus furiosus |
1.1.1.42 | 90 | - |
- |
Thermotoga maritima |
1.1.1.42 | 95 | - |
above | Aeropyrum pernix |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | additional information | - |
thermal denaturation is an irreversible process | Pyrococcus furiosus |
1.1.1.41 | 104 | - |
the Tm-value is 103.7°C | Pyrococcus furiosus |
1.1.1.42 | additional information | - |
thermal denaturation is an irreversible process | Thermotoga maritima |
1.1.1.42 | additional information | - |
thermal denaturation is an irreversible process | Aeropyrum pernix |
1.1.1.42 | 98 | - |
the Tm-value is 98.3°C | Thermotoga maritima |
1.1.1.42 | 110 | - |
the Tm-value is 109.9°C | Aeropyrum pernix |