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Literature summary extracted from

  • Haney, P.J.; Stees, M.; Konisky, J.
    Analysis of thermal stabilizing interactions in mesophilic and thermophilic adenylate kinases from the genus Methanococcus (1999), J. Biol. Chem., 274, 28453-28458.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.3 overexpression in Escherichia coli Methanothermococcus thermolithotrophicus
2.7.4.3 overexpression in Escherichia coli Methanotorris igneus
2.7.4.3 overexpression in Escherichia coli, wild-type enzyme, mutant enzymes and chimeric enzymes Methanocaldococcus jannaschii
2.7.4.3 overexpression in Escherichia colii, wild-type enzyme, mutant enzymes and chimeric enzymes Methanococcus voltae

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Methanocaldococcus jannaschii P43409
-
-
2.7.4.3 Methanocaldococcus jannaschii DSM 2661 P43409
-
-
2.7.4.3 Methanococcus voltae P43411
-
-
2.7.4.3 Methanothermococcus thermolithotrophicus
-
-
-
2.7.4.3 Methanotorris igneus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3
-
 Methanothermococcus thermolithotrophicus
2.7.4.3
-
 Methanococcus voltae
2.7.4.3
-
 Methanocaldococcus jannaschii
2.7.4.3
-
 Methanotorris igneus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ATP + AMP
-
 Methanothermococcus thermolithotrophicus 2 ADP
-
 ?
2.7.4.3 ATP + AMP
-
 Methanococcus voltae 2 ADP
-
 ?
2.7.4.3 ATP + AMP
-
 Methanocaldococcus jannaschii 2 ADP
-
 ?
2.7.4.3 ATP + AMP
-
 Methanotorris igneus 2 ADP
-
 ?
2.7.4.3 ATP + AMP
-
 Methanocaldococcus jannaschii DSM 2661 2 ADP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.3 37
-
 wild-type enzyme Methanococcus voltae
2.7.4.3 45
-
 mutant enzyme J36V Methanococcus voltae
2.7.4.3 53
-
 mutant enzyme V160J Methanococcus voltae
2.7.4.3 68
-
 wild-type enzyme Methanothermococcus thermolithotrophicus
2.7.4.3 72
-
 mutant enzyme V36J Methanocaldococcus jannaschii
2.7.4.3 75
-
 mutant enzyme J160V Methanocaldococcus jannaschii
2.7.4.3 80
-
 wild-type enzyme Methanotorris igneus
2.7.4.3 83
-
 wild-type enzyme Methanocaldococcus jannaschii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.4.3 25 60 25°C: about 85% of maximal activity, 60°C: about 60% of maximal activity, wilde-type enzyme Methanococcus voltae
2.7.4.3 60 90 60°C: about 50% of amximal activity, 90°C: about 89% of maximal activity Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.3 69
-
 Tm-value, wild-type enzyme Methanococcus voltae
2.7.4.3 73
-
 Tm-value, mutant enzyme J36V Methanococcus voltae
2.7.4.3 74
-
 Tm-value, mutant enzyme V160J Methanococcus voltae
2.7.4.3 86
-
 Tm-value, wild-type enzyme Methanothermococcus thermolithotrophicus
2.7.4.3 95
-
 tm-value is above 95°C Methanotorris igneus
2.7.4.3 96
-
 Tm-value, mutant enzyme V160J Methanocaldococcus jannaschii
2.7.4.3 98
-
 Tm-value, mutant enzyme J36V Methanocaldococcus jannaschii
2.7.4.3 103
-
 Tm-value, wild-type enzyme Methanocaldococcus jannaschii