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Literature summary extracted from

  • VanNice, J.C.; Skaff, D.A.; Wyckoff, G.J.; Miziorko, H.M.
    Expression in Haloferax volcanii of 3-hydroxy-3-methylglutaryl coenzyme A synthase facilitates isolation and characterization of the active form of a key enzyme required for polyisoprenoid cell membrane biosynthesis in halophilic archaea (2013), J. Bacteriol., 195, 3854-3862.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.10 expression in Haloferax volcanii Haloferax volcanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 hymeglusin
-
 Haloferax volcanii
2.3.3.10 methanol
-
 Haloferax volcanii
2.3.3.10 additional information insensitive to feedback substrate inhibition by acetoacetyl-CoA Haloferax volcanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.0014
-
 acetoacetyl-CoA pH 8.0, 30°C Haloferax volcanii
2.3.3.10 0.05
-
 acetyl-CoA pH 8.0, 30°C Haloferax volcanii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.10 KCl optimal concentration Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.10 51975
-
 x * 51975, matrix-assisted laser desorption ionization-time of flight mass spectrometry Haloferax volcanii
2.3.3.10 65000
-
 x * 65000, SDS-PAGE Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + H2O + acetoacetyl-CoA Haloferax volcanii the enzyme is involved in the mevalonate pathway. Archaea are characterized by cell membrane lipids that contain, as a major component, polyisoprenoids in ether linkages to glycerol rather than the ester-linked fatty acids that characterize membranes of other organisms. Archaeal polyisoprenoids derive from isopentenyl 5-diphosphate produced by enzymes of the mevalonate pathway (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + H2O + acetoacetyl-CoA Haloferax volcanii DSM 3757 the enzyme is involved in the mevalonate pathway. Archaea are characterized by cell membrane lipids that contain, as a major component, polyisoprenoids in ether linkages to glycerol rather than the ester-linked fatty acids that characterize membranes of other organisms. Archaeal polyisoprenoids derive from isopentenyl 5-diphosphate produced by enzymes of the mevalonate pathway (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Haloferax volcanii D4GWR6
-
-
2.3.3.10 Haloferax volcanii DSM 3757 D4GWR6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10
-
 Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + H2O + acetoacetyl-CoA
-
 Haloferax volcanii (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + H2O + acetoacetyl-CoA the enzyme is involved in the mevalonate pathway. Archaea are characterized by cell membrane lipids that contain, as a major component, polyisoprenoids in ether linkages to glycerol rather than the ester-linked fatty acids that characterize membranes of other organisms. Archaeal polyisoprenoids derive from isopentenyl 5-diphosphate produced by enzymes of the mevalonate pathway Haloferax volcanii (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + H2O + acetoacetyl-CoA
-
 Haloferax volcanii DSM 3757 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?
2.3.3.10 acetyl-CoA + H2O + acetoacetyl-CoA the enzyme is involved in the mevalonate pathway. Archaea are characterized by cell membrane lipids that contain, as a major component, polyisoprenoids in ether linkages to glycerol rather than the ester-linked fatty acids that characterize membranes of other organisms. Archaeal polyisoprenoids derive from isopentenyl 5-diphosphate produced by enzymes of the mevalonate pathway Haloferax volcanii DSM 3757 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.10 ? x * 65000, SDS-PAGE Haloferax volcanii
2.3.3.10 ? x * 51975, matrix-assisted laser desorption ionization-time of flight mass spectrometry Haloferax volcanii

Synonyms

EC Number Synonyms Comment Organism
2.3.3.10 3-hydroxy-3-methylglutaryl CoA synthase
-
 Haloferax volcanii
2.3.3.10 3-hydroxy-3-methylglutaryl coenzyme A synthase
-
 Haloferax volcanii
2.3.3.10 HMGCS
-
 Haloferax volcanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.10 30
-
 assay at Haloferax volcanii
2.3.3.10 45
-
-
 Haloferax volcanii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.10 45
-
 in the presence of an excess of acetyl-CoA, the half-life of the active enzyme is 27 h Haloferax volcanii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.10 4.6
-
 acetyl-CoA pH 8.0, 30°C Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.10 8
-
 assay at Haloferax volcanii
2.3.3.10 8.5
-
-
 Haloferax volcanii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.10 0.00057
-
 hymeglusin 18°C, pH 8 Haloferax volcanii

General Information

EC Number General Information Comment Organism
2.3.3.10 physiological function the enzyme is involved in the mevalonate pathway in archaea. Archaea are characterized by cell membrane lipids that contain, as a major component, polyisoprenoids in ether linkages to glycerol rather than the ester-linked fatty acids that characterize membranes of other organisms. Archaeal polyisoprenoids derive from isopentenyl 5-diphosphate produced by enzymes of the mevalonate pathway Haloferax volcanii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.3.10 92
-
 acetyl-CoA pH 8.0, 30°C Haloferax volcanii