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Literature summary extracted from

  • Terlesky, K.C.; Nelson, M.J.; Ferry, J.G.
    Isolation of an enzyme complex with carbon monoxide dehydrogenase activity containing corrinoid and nickel from acetate-grown Methanosarcina thermophila (1986), J. Bacteriol., 168, 1053-1058.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.4 corrinoid cofactor the multifunctional enzyme complex contains a corrinoid cofactor Methanosarcina thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.4 KCN
-
 Methanosarcina thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.4 Co2+ the multifunctional enzyme complex contains Co2+ Methanosarcina thermophila
1.2.7.4 Fe-S center the multifunctional enzyme complex contains iron-sulfur centers Methanosarcina thermophila
1.2.7.4 Ni2+ the multifunctional enzyme complex contains Ni2+ Methanosarcina thermophila
1.2.7.4 Zn2+ the multifunctional enzyme complex contains Zn2+ Methanosarcina thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.4 19000
-
 x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE Methanosarcina thermophila
1.2.7.4 58000
-
 x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE Methanosarcina thermophila
1.2.7.4 60000
-
 x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE Methanosarcina thermophila
1.2.7.4 71000
-
 x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE Methanosarcina thermophila
1.2.7.4 89000
-
 x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE Methanosarcina thermophila
1.2.7.4 250000
-
 the native enzyme (MW 250000 Da) forms aggregates with an MW of approximately 1000000, gradient gel electrophoresis Methanosarcina thermophila
1.2.7.4 1000000
-
 the native enzyme (MW 250000 Da) forms aggregates with an MW of approximately 1000000, gradient gel electrophoresis Methanosarcina thermophila

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Methanosarcina thermophila
-
-
-
1.2.7.4 Methanosarcina thermophila TM-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.4
-
 Methanosarcina thermophila

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.7.4 culture condition:acetate-grown cell activity of one of the CO dehydrogenases is sixfold greater in acetate-grown compared with methanol-grown cells Methanosarcina thermophila
-
1.2.7.4 culture condition:methanol-grown cell activity of one of the CO dehydrogenases is sixfold greater in acetate-grown compared with methanol-grown cells Methanosarcina thermophila
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + FAD at 15% of the specific activity compared to methyl viologen Methanosarcina thermophila CO2 + FADH2
-
 ?
1.2.7.4 CO + H2O + FAD at 15% of the specific activity compared to methyl viologen Methanosarcina thermophila TM-1 CO2 + FADH2
-
 ?
1.2.7.4 CO + H2O + FMN at 15% of the specific activity compared to methyl viologen Methanosarcina thermophila CO2 + FMNH2
-
 ?
1.2.7.4 CO + H2O + FMN at 15% of the specific activity compared to methyl viologen Methanosarcina thermophila TM-1 CO2 + FMNH2
-
 ?
1.2.7.4 CO + H2O + oxidized methyl viologen no enzyme with cofactor F420 Methanosarcina thermophila CO2 + reduced methyl viologen
-
 ?
1.2.7.4 CO + H2O + oxidized methyl viologen no enzyme with cofactor F420 Methanosarcina thermophila TM-1 CO2 + reduced methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.4 ? x * 89000 + x * 71000 + x * 60000 + x * 58000 + x * 19000, SDS-PAGE Methanosarcina thermophila

Synonyms

EC Number Synonyms Comment Organism
1.2.7.4 CO dehydrogenase
-
 Methanosarcina thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.4 23
-
 assay at Methanosarcina thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.4 8.4
-
 assay with methyl viologen Methanosarcina thermophila