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Literature summary extracted from

  • Li, X.; Yu, H.Y.
    Characterization of a halostable endoglucanase with organic solvent-tolerant property from Haloarcula sp. G10 (2013), Int. J. Biol. Macromol., 62, 101-106.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 Benzene enhances enzyme activity Haloarcula sp.
3.2.1.4 n-hexane enhances enzyme activity Haloarcula sp.

Application

EC Number Application Comment Organism
3.2.1.4 biotechnology considering its thermostable, alkali-stable, halostable and organic solvent-tolerant properties, the enzyme might be potentially useful for future applications in biotechnological processes Haloarcula sp.

General Stability

EC Number General Stability Organism
3.2.1.4 halostable, the enzyme retains more than 80% activity after incubation with 10–30% NaCl for 24 h Haloarcula sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 diethyl dicarbonate 10 mM, 17.6% residual activity Haloarcula sp.
3.2.1.4 EDTA 10 mM, 11.2% residual activity Haloarcula sp.
3.2.1.4 Hg2+ 5 mM, 58% inhibition Haloarcula sp.
3.2.1.4 phenylmethylsulfonyl fluoride 10 mM, 18.1% residual activity Haloarcula sp.
3.2.1.4 SDS 10 mM, 49.2% residual activity Haloarcula sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 cellulose Km-value for carboxymethylcellulose-Na as substrate is 3.01 mg/ml Haloarcula sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 extracellular
-
 Haloarcula sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+ 5 mM, 1.3fold stimulation Haloarcula sp.
3.2.1.4 Mg2+ 5 mM, 1.5fold stimulation Haloarcula sp.
3.2.1.4 NaCl optimal activity at 17.5% NaCl Haloarcula sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 36000
-
 gel filtration Haloarcula sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.4 1-butanol decreases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.
3.2.1.4 1-decanol increases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.
3.2.1.4 Acetone decreases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.
3.2.1.4 acetonitrile decreases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.
3.2.1.4 benzene increases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.
3.2.1.4 chloroform no effect on stability Haloarcula sp.
3.2.1.4 cyclohexane increases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.
3.2.1.4 DMSO decreases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.
3.2.1.4 Ethanol decreases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.
3.2.1.4 isooctane increases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.
3.2.1.4 Methanol decreases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.
3.2.1.4 additional information high activity and stability in the presence of non-polar hydrophobic organic solvents Haloarcula sp.
3.2.1.4 n-hexane increases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.
3.2.1.4 phenylmethylsulfonyl fluoride decreases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.
3.2.1.4 toluene increases stability of the enzyme as compared to activity in the absence of organic solvents Haloarcula sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Haloarcula sp.
-
-
-
3.2.1.4 Haloarcula sp. G10
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Haloarcula sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 71.2
-
 pH 9.0, 50°C Haloarcula sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 cellulose + H2O
-
 Haloarcula sp. ?
-
 ?
3.2.1.4 cellulose + H2O
-
 Haloarcula sp. G10 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 monomer 1 * 36000, SDS-PAGE Haloarcula sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
-
 Haloarcula sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 20 80 20°C: about 40% of maximal activity, 80°C: about 80% of maximal activity Haloarcula sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 80
-
 24 h incubation, enzyme retains more than 60% activity Haloarcula sp.
3.2.1.4 90
-
 24 h incubation, complete loss of activity Haloarcula sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 9
-
-
 Haloarcula sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 6 11 pH 6.0: about 80% of maximal activity, pH 11: about 70% of maximal activity Haloarcula sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 7 11 good stability in the pH range from7.0–11.0 Haloarcula sp.