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Literature summary extracted from

  • Diaz, M.E.; Mayoral, J.G.; Priestap, H.; Nouzova, M.; Rivera-Perez, C.; Noriega, F.G.
    Characterization of an isopentenyl diphosphate isomerase involved in the juvenile hormone pathway in Aedes aegypti (2012), Insect Biochem. Mol. Biol., 42, 751-757.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.2 expressed in Escherichia coli BL21(DE3) cells Aedes aegypti

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.3.2 iodoacetamide 70% inhibition at 0.001 mM, complete inhibition at 0.1 mM Aedes aegypti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.2 0.2768
-
 isopentenyl diphosphate in 0.4 M Tris, 1 mM dithiothreitol, 10 mM MgCl2, pH 8.0, at 50°C Aedes aegypti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.3.2 Mg2+ required, 2fold increase in activity with 10 mM Mg2+ Aedes aegypti
5.3.3.2 Mn2+ required, 2.5fold increase in activity with 0.1 mM Mn2+ Aedes aegypti
5.3.3.2 additional information not activated by Zn2+ Aedes aegypti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.2 28500
-
 x * 28500, calculated from amino acid sequence Aedes aegypti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.2 isopentenyl diphosphate Aedes aegypti
-
 dimethylallyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.2 Aedes aegypti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.2 cobalt column chromatography Aedes aegypti

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.3.2 corpus allatum
-
 Aedes aegypti
-
5.3.3.2 corpus cardiacum
-
 Aedes aegypti
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.2 isopentenyl diphosphate
-
 Aedes aegypti dimethylallyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.3.2 ? x * 28500, calculated from amino acid sequence Aedes aegypti

Synonyms

EC Number Synonyms Comment Organism
5.3.3.2 IPPI
-
 Aedes aegypti
5.3.3.2 isopentenyl diphosphate isomerase
-
 Aedes aegypti