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Literature summary extracted from

  • Sen, S.E.; Tomasello, A.; Grasso, M.; Denton, R.; Macor, J.; Beliveau, C.; Cusson, M.; Crowell, D.N.
    Cloning, expression and characterization of lepidopteran isopentenyl diphosphate isomerase (2012), Insect Biochem. Mol. Biol., 42, 739-750.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.2 expressed in Escherichia coli Rosetta cells Manduca sexta
5.3.3.2 expressed in Escherichia coli Rosetta cells Choristoneura fumiferana

Protein Variants

EC Number Protein Variants Comment Organism
5.3.3.2 C113S inactive Choristoneura fumiferana
5.3.3.2 C113S/114S inactive Choristoneura fumiferana
5.3.3.2 C114S inactive Choristoneura fumiferana
5.3.3.2 E169/171Q inactive Choristoneura fumiferana
5.3.3.2 E169Q inactive Choristoneura fumiferana
5.3.3.2 E171Q inactive Choristoneura fumiferana
5.3.3.2 W216F 10% activity compared to the wild type enzyme Choristoneura fumiferana
5.3.3.2 Y105F 115% activity compared to the wild type enzyme Choristoneura fumiferana

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.3.2 (2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate
-
Choristoneura fumiferana
5.3.3.2 (2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate
-
Manduca sexta
5.3.3.2 (2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate
-
Sus scrofa
5.3.3.2 (2Z)-3-methylhex-2-en-1-yl trihydrogen diphosphate
-
Choristoneura fumiferana
5.3.3.2 (2Z)-3-methylhex-2-en-1-yl trihydrogen diphosphate
-
Manduca sexta
5.3.3.2 (2Z)-3-methylhex-2-en-1-yl trihydrogen diphosphate
-
Sus scrofa
5.3.3.2 (2Z)-3-methyloct-2-en-1-yl trihydrogen diphosphate
-
Choristoneura fumiferana
5.3.3.2 (2Z)-3-methyloct-2-en-1-yl trihydrogen diphosphate
-
Manduca sexta
5.3.3.2 (2Z)-3-methyloct-2-en-1-yl trihydrogen diphosphate
-
Sus scrofa
5.3.3.2 2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate
-
Choristoneura fumiferana
5.3.3.2 2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate
-
Manduca sexta
5.3.3.2 2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate
-
Sus scrofa
5.3.3.2 2-[ethyl(propyl)amino]ethyl trihydrogen diphosphate
-
Choristoneura fumiferana
5.3.3.2 2-[ethyl(propyl)amino]ethyl trihydrogen diphosphate
-
Manduca sexta
5.3.3.2 dimethylallyl diphosphate
-
Choristoneura fumiferana
5.3.3.2 dimethylallyl diphosphate
-
Manduca sexta
5.3.3.2 dimethylallyl diphosphate
-
Sus scrofa
5.3.3.2 dimethylammonium diphosphate
-
Choristoneura fumiferana
5.3.3.2 dimethylammonium diphosphate
-
Manduca sexta
5.3.3.2 dimethylammonium diphosphate
-
Sus scrofa
5.3.3.2 homodimethylallyl diphosphate
-
Choristoneura fumiferana
5.3.3.2 homodimethylallyl diphosphate
-
Manduca sexta
5.3.3.2 homodimethylallyl diphosphate
-
Sus scrofa
5.3.3.2 homoisopentenyl diphosphate
-
Choristoneura fumiferana
5.3.3.2 homoisopentenyl diphosphate
-
Manduca sexta
5.3.3.2 homoisopentenyl diphosphate
-
Sus scrofa
5.3.3.2 additional information not inhibited by 2-[ethyl(propyl)amino]ethyl trihydrogen diphosphate Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.2 0.0033
-
isopentenyl diphosphate wild type enzyme, in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana
5.3.3.2 0.006
-
isopentenyl diphosphate mutant enzyme E169Q, in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana
5.3.3.2 0.006
-
isopentenyl diphosphate mutant enzyme Y105F, in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana
5.3.3.2 0.0116
-
isopentenyl diphosphate mutant enzyme W216F, in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana
5.3.3.2 0.0128
-
isopentenyl diphosphate mutant enzyme Y159F, in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.3.2 mitochondrion
-
Sus scrofa 5739
-
5.3.3.2 mitochondrion
-
Manduca sexta 5739
-
5.3.3.2 mitochondrion
-
Choristoneura fumiferana 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.3.2 Mg2+ preference for Mn2+ over Mg2+ Sus scrofa
5.3.3.2 Mg2+ preference for Mn2+ over Mg2+ Manduca sexta
5.3.3.2 Mg2+ preference for Mn2+ over Mg2+ Choristoneura fumiferana
5.3.3.2 Mn2+ preference for Mn2+ over Mg2+ Sus scrofa
5.3.3.2 Mn2+ preference for Mn2+ over Mg2+ Manduca sexta
5.3.3.2 Mn2+ preference for Mn2+ over Mg2+ Choristoneura fumiferana
5.3.3.2 additional information not activated by Zn2*, Fe2*, or Cu2+ Choristoneura fumiferana
5.3.3.2 additional information not activated by Zn2+, Fe2+, or Cu2+ Manduca sexta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.2 29200
-
x * 29200, calculated from amino acid sequence Manduca sexta
5.3.3.2 29200
-
x * 29200, calculated from amino acid sequence Choristoneura fumiferana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.2 isopentenyl diphosphate Sus scrofa
-
dimethylallyl diphosphate
-
?
5.3.3.2 isopentenyl diphosphate Manduca sexta
-
dimethylallyl diphosphate
-
?
5.3.3.2 isopentenyl diphosphate Choristoneura fumiferana
-
dimethylallyl diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.2 Choristoneura fumiferana
-
-
-
5.3.3.2 Manduca sexta
-
-
-
5.3.3.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.2 TALON metal affinity resin column chromatography Manduca sexta
5.3.3.2 TALON metal affinity resin column chromatography Choristoneura fumiferana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.3.2 corpus allatum highly abundant in the corpora allata Manduca sexta
-
5.3.3.2 liver
-
Sus scrofa
-
5.3.3.2 midgut
-
Choristoneura fumiferana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.2 isopentenyl diphosphate
-
Sus scrofa dimethylallyl diphosphate
-
?
5.3.3.2 isopentenyl diphosphate
-
Manduca sexta dimethylallyl diphosphate
-
?
5.3.3.2 isopentenyl diphosphate
-
Choristoneura fumiferana dimethylallyl diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
5.3.3.2 ? x * 29200, calculated from amino acid sequence Manduca sexta
5.3.3.2 ? x * 29200, calculated from amino acid sequence Choristoneura fumiferana

Synonyms

EC Number Synonyms Comment Organism
5.3.3.2 IPPI
-
Sus scrofa
5.3.3.2 IPPI
-
Manduca sexta
5.3.3.2 IPPI
-
Choristoneura fumiferana
5.3.3.2 isopentenyl diphosphate isomerase
-
Sus scrofa
5.3.3.2 isopentenyl diphosphate isomerase
-
Manduca sexta
5.3.3.2 isopentenyl diphosphate isomerase
-
Choristoneura fumiferana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.3.2 Manduca sexta calculated from amino acid sequence
-
6
5.3.3.2 Choristoneura fumiferana calculated from amino acid sequence
-
6.4

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.3.3.2 0.0008
-
in 50 mM Tris-HCl (pH 7.0), at 37°C Sus scrofa dimethylammonium diphosphate
5.3.3.2 0.003
-
in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana 2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate
5.3.3.2 0.0055
-
in 50 mM Tris-HCl (pH 7.0), at 37°C Sus scrofa 2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate
5.3.3.2 0.007
-
in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana 2-[ethyl(propyl)amino]ethyl trihydrogen diphosphate
5.3.3.2 0.066
-
in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana homoisopentenyl diphosphate
5.3.3.2 0.092
-
in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana dimethylallyl diphosphate
5.3.3.2 0.131
-
in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana homodimethylallyl diphosphate
5.3.3.2 0.156
-
in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana (2Z)-3-methylhex-2-en-1-yl trihydrogen diphosphate
5.3.3.2 0.158
-
in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana (2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate
5.3.3.2 0.234
-
in 25 mM Tris-HCl, pH 7.0, at 37°C Choristoneura fumiferana (2Z)-3-methyloct-2-en-1-yl trihydrogen diphosphate