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Literature summary extracted from

  • Testa, F.; Mastronicola, D.; Cabelli, D.E.; Bordi, E.; Pucillo, L.P.; Sarti, P.; Saraiva, L.M.; Giuffre, A.; Teixeira, M.
    The superoxide reductase from the early diverging eukaryote Giardia intestinalis (2011), Free Radic. Biol. Med., 51, 1567-1574.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.2 expression in Escherichia coli strain BL21-Gold (DE3) Giardia intestinalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.2 Fe2+ one Fe per monomer, consistent with full occupancy of the metal center in the active site of the enzyme Giardia intestinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.2 reduced rubredoxin + superoxide + 2 H+ Giardia intestinalis
-
 rubredoxin + H2O2
-
 ?
1.15.1.2 reduced rubredoxin + superoxide + 2 H+ Giardia intestinalis WB clone C6
-
 rubredoxin + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 no activity in Giardia intestinalis
-
-
-
1.15.1.2 Giardia intestinalis
-
-
-
1.15.1.2 Giardia intestinalis WB clone C6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.2 recombinant His-tagged enzyme to homogeneity from Escherichia coli strain BL21-Gold (DE3) by nickel affinity chromatography, gel filtration and cleavage of the His-tag, followed by a second time nickel affinity chromatography and gel filtration Giardia intestinalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.2 trophozoite
-
 Giardia intestinalis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.2 reduced rubredoxin + superoxide + 2 H+
-
 Giardia intestinalis rubredoxin + H2O2
-
 ?
1.15.1.2 reduced rubredoxin + superoxide + 2 H+
-
 Giardia intestinalis WB clone C6 rubredoxin + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.2 tetramer
-
 Giardia intestinalis

Synonyms

EC Number Synonyms Comment Organism
1.15.1.2 SOR
-
 Giardia intestinalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.2 10
-
 assay at Giardia intestinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.2 7 7.5
-
 Giardia intestinalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.15.1.2 rubredoxin rubredoxins, small proteins with a [FeCys4] center known to be involved in electron transfer processes, are generally assumed to be the direct electron donors to SOR, based on the fact that the genes encoding rubredoxin and SOR lie in the same operon in some bacteria. Consistently, reduced rubredoxins are shown to reduce both 1Fe- and 2Fe-SORs, but physiological electron donors other than rubredoxins must exist because rubredoxins are missing in a large number of organisms that encode SORs Giardia intestinalis

General Information

EC Number General Information Comment Organism
1.15.1.2 evolution Giardia trophozoite expresses an enzyme probably acquired from a prokaryote by lateral gene transfer. Rubredoxins, small proteins with a [FeCys4] center known to be involved in electron transfer processes, are generally assumed to be the direct electron donors to SOR, based on the fact that the genes encoding rubredoxin and SOR lie in the same operon in some bacteria. Consistently, reduced rubredoxins are shown to reduce both 1Fe- and 2Fe-SORs, but physiological electron donors other than rubredoxins must exist because rubredoxins are missing in a large number of organisms that encode SORs Giardia intestinalis
1.15.1.2 additional information pH dependent ligand exchange in the final intermediate, overview Giardia intestinalis
1.15.1.2 physiological function Giardia trophozoite expresses a SOR possibly involved in superoxide detoxification Giardia intestinalis