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Literature summary extracted from

  • Yang, R.; Yin, Y.; Guo, Q.; Gu, Z.
    Purification, properties and cDNA cloning of glutamate decarboxylase in germinated faba bean (Vicia faba L.) (2013), Food Chem., 138, 1945-1951.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.15 gene gad, cloning from germinated faba bean, DNA and amino acid sequence determination and analysis, sequence comparisons Vicia faba

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.15 2-mercaptoethanol
-
 Vicia faba
4.1.1.15 Aminoxyacetate
-
 Vicia faba
4.1.1.15 Ba2+
-
 Vicia faba
4.1.1.15 Ca2+ activates at 0.2 mM, slight inhibition above 1 mM Vicia faba
4.1.1.15 Cu2+ strong inhibition at 3 mM Vicia faba
4.1.1.15 EGTA
-
 Vicia faba
4.1.1.15 Fe3+
-
 Vicia faba
4.1.1.15 L-cysteine
-
 Vicia faba
4.1.1.15 Mg2+
-
 Vicia faba
4.1.1.15 Na2EDTA
-
 Vicia faba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.15 2.63
-
 L-glutamate pH 6.0, 40°C, recombinant enzyme Vicia faba

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.15 Ca2+ activates at 0.2 mM, slight inhibition above 1 mM Vicia faba

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.15 57740
-
 x * 57740, sequence calculation, x * 58000, SDS-PAGE Vicia faba
4.1.1.15 58000
-
 x * 57740, sequence calculation, x * 58000, SDS-PAGE Vicia faba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.15 L-glutamate Vicia faba
-
 4-aminobutanoate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Vicia faba M1GCP8 cv. Qidou 2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.15 native enzyme 32fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography Vicia faba

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.15 21.44
-
 purified native enzyme, pH 6.0, 40°C Vicia faba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
 Vicia faba 4-aminobutanoate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.15 ? x * 57740, sequence calculation, x * 58000, SDS-PAGE Vicia faba

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 GAD
-
 Vicia faba

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.15 40
-
 recombinant enzyme Vicia faba

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.15 20 60 activity range, profile overview, inactivation above Vicia faba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.15 6
-
 recombinant enzyme Vicia faba

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.15 3 9 activity range, profile overview Vicia faba

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.15 Vicia faba sequence calculation
-
 5.41