EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.3 | biofuel production | biodiesel production | Haloarcula sp. |
EC Number | General Stability | Organism |
---|---|---|
3.1.1.3 | highly stable in the presence of 2.5-25% NaCl | Haloarcula sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | 2-mercaptoethanol | 10 mM, 7.7% inhibition | Haloarcula sp. | |
3.1.1.3 | Cu2+ | 1 mM, 19% inhibition | Haloarcula sp. | |
3.1.1.3 | diethyldicarbonate | 10 mM, 17% inhibition | Haloarcula sp. | |
3.1.1.3 | EDTA | 10 mM, 92% inhibition | Haloarcula sp. | |
3.1.1.3 | Hg2+ | 1 mM, 89% inhibition | Haloarcula sp. | |
3.1.1.3 | PMSF | 10 mM, 89% inhibition | Haloarcula sp. | |
3.1.1.3 | poly-alpha-olefin | 10 mM, 81% inhibition | Haloarcula sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.3 | extracellular | - |
Haloarcula sp. | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Ca2+ | 1 mM, 1.3fold stimulation | Haloarcula sp. | |
3.1.1.3 | Mg2+ | 1 mM, 1.3fold stimulation | Haloarcula sp. | |
3.1.1.3 | NaCl | no activity is detected in the absence of NaCl, optimal activity at 15% | Haloarcula sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 45000 | - |
gel filtration | Haloarcula sp. |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.1.3 | 1-decanol | 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | Acetone | 30% (v/v), half-life: 1 day, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | acetonitrile | 30% (v/v), half-life: less than 1 day, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | benzene | 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | chloroform | 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | cyclohexane | 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | dimethylformamide | 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | DMSO | 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | Ethanol | 30% (v/v), half-life: less than 1 day, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | Glycerol | 30% (v/v), half-life: 4 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | isooctane | 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | Methanol | 30% (v/v), half-life: 2 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | n-hexane | 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | tert-Butanol | 30% (v/v), half-life: 1 day, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
3.1.1.3 | toluene | 30% (v/v), half-life: more than 5 days, the half-life of the enzyme in absence of organic solvents is 3 days | Haloarcula sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Haloarcula sp. | - |
- |
- |
3.1.1.3 | Haloarcula sp. G41 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.3 | - |
Haloarcula sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.3 | culture supernatant | - |
Haloarcula sp. | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | 4-nitrophenyl acetate + H2O | 12% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + acetate | - |
? | |
3.1.1.3 | 4-nitrophenyl acetate + H2O | 12% of the activity with 4-nitrophenyl myristate | Haloarcula sp. G41 | 4-nitrophenol + acetate | - |
? | |
3.1.1.3 | 4-nitrophenyl butyrate + H2O | 20% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + butyrate | - |
? | |
3.1.1.3 | 4-nitrophenyl butyrate + H2O | 20% of the activity with 4-nitrophenyl myristate | Haloarcula sp. G41 | 4-nitrophenol + butyrate | - |
? | |
3.1.1.3 | 4-nitrophenyl decanoate + H2O | 85% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + decanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl decanoate + H2O | 85% of the activity with 4-nitrophenyl myristate | Haloarcula sp. G41 | 4-nitrophenol + decanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl hexanoate + H2O | 30% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + hexanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl hexanoate + H2O | 30% of the activity with 4-nitrophenyl myristate | Haloarcula sp. G41 | 4-nitrophenol + hexanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl laurate + H2O | 95% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + laurate | - |
? | |
3.1.1.3 | 4-nitrophenyl myristate + H2O | maximal activity among the tested 4-nitrophenyl esters | Haloarcula sp. | 4-nitrophenol + myristate | - |
? | |
3.1.1.3 | 4-nitrophenyl octanoate + H2O | 70% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + octanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl octanoate + H2O | 70% of the activity with 4-nitrophenyl myristate | Haloarcula sp. G41 | 4-nitrophenol + octanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl palmitate + H2O | 95% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + palmitate | - |
? | |
3.1.1.3 | 4-nitrophenyl stearate + H2O | 90% of the activity with 4-nitrophenyl myristate | Haloarcula sp. | 4-nitrophenol + stearate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.3 | monomer | 1 * 45000, SDS-PAGE | Haloarcula sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 70 | - |
- |
Haloarcula sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 30 | 80 | 30°C: about 70% of maximal activity, 80°C: about 70% of maximal activity | Haloarcula sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 60 | - |
24 h, stable | Haloarcula sp. |
3.1.1.3 | 90 | - |
24 h, 40% loss of activity | Haloarcula sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 8 | - |
- |
Haloarcula sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 6 | 11 | pH 6.0: about 55% of maximal activity, pH 11.0: about 50% of maximal activity | Haloarcula sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 7 | 11 | ighly stable in the pH range of 7.011.0 after 24 h incubation | Haloarcula sp. |