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Literature summary extracted from

  • Yu, Z.L.; Liu, J.; Wang, F.Q.; Dai, M.; Zhao, B.H.; He, J.G.; Zhang, H.
    Cloning and characterization of a novel CoA-ligase gene from Penicillium chrysogenum (2011), Folia Microbiol. (Praha), 56, 246-252.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.30 gene phlC, cloning from DNA library in lambda ZAP II, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His8-tagged enzyme in Escherichia coli strain BL21 (DE3)-RP, subcloning in Escherichia coli strain DH5alpha, real-time quantitative RT-PCR expression analysis Penicillium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.30 additional information
-
 additional information Michaelis-Menten kinetics Penicillium chrysogenum
6.2.1.30 0.026
-
 caproate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.046
-
 caprylate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.07
-
 caprate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.092
-
 palmitate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.257
-
 Phenoxyacetate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.809
-
 phenylacetate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.841
-
 stearate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.30 peroxisome the enzyme contains a peroxisomal targeting signal 1, A-K-L, on its C terminus, indicating its peroxisomal location Penicillium chrysogenum 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.30 Mg2+ required Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.30 61635
-
 x * 61635, sequence calculation Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.30 ATP + phenylacetate + CoA Penicillium chrysogenum
-
 AMP + diphosphate + phenylacetyl-CoA
-
 ?
6.2.1.30 ATP + phenylacetate + CoA Penicillium chrysogenum WIS 54-1255
-
 AMP + diphosphate + phenylacetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.30 Penicillium chrysogenum D3GE78 gene phlC
-
6.2.1.30 Penicillium chrysogenum WIS 54-1255 D3GE78 gene phlC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.30 recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 (DE3)-RP by nickel affinity chromatography Penicillium chrysogenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.30 0.00013
-
 purified recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.30 ATP + 4-coumarate + CoA reaction of EC 6.2.1.12 Penicillium chrysogenum AMP + diphosphate + 4-coumaryl-CoA
-
 ?
6.2.1.30 ATP + caprate + CoA
-
 Penicillium chrysogenum AMP + diphosphate + capryl-CoA
-
 ?
6.2.1.30 ATP + caproate + CoA best substrate Penicillium chrysogenum AMP + diphosphate + caproyl-CoA
-
 ?
6.2.1.30 ATP + caprylate + CoA
-
 Penicillium chrysogenum AMP + diphosphate + caprylyl-CoA
-
 ?
6.2.1.30 ATP + cinnamate + CoA reaction of EC 6.2.1.12 Penicillium chrysogenum AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.30 ATP + cinnamate + CoA reaction of EC 6.2.1.12 Penicillium chrysogenum WIS 54-1255 AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.30 ATP + palmitate + CoA low activity, reaction of EC 6.2.1.3 Penicillium chrysogenum AMP + diphosphate + palmityl-CoA
-
 ?
6.2.1.30 ATP + phenoxyacetate + CoA low activity Penicillium chrysogenum AMP + diphosphate + phenoxyacetyl-CoA
-
 ?
6.2.1.30 ATP + phenoxyacetate + CoA low activity Penicillium chrysogenum WIS 54-1255 AMP + diphosphate + phenoxyacetyl-CoA
-
 ?
6.2.1.30 ATP + phenylacetate + CoA
-
 Penicillium chrysogenum AMP + diphosphate + phenylacetyl-CoA
-
 ?
6.2.1.30 ATP + phenylacetate + CoA low activity Penicillium chrysogenum AMP + diphosphate + phenylacetyl-CoA
-
 ?
6.2.1.30 ATP + phenylacetate + CoA
-
 Penicillium chrysogenum WIS 54-1255 AMP + diphosphate + phenylacetyl-CoA
-
 ?
6.2.1.30 ATP + phenylacetate + CoA low activity Penicillium chrysogenum WIS 54-1255 AMP + diphosphate + phenylacetyl-CoA
-
 ?
6.2.1.30 ATP + stearate + CoA very low activity, reaction of EC 6.2.1.3 Penicillium chrysogenum AMP + diphosphate + stearyl-CoA
-
 ?
6.2.1.30 additional information the enzyme displays broad substrate spectrum and shows higher activities to medium and long-chain fatty acids. The enzyme is also active with cinnamic acid and coumaric acid, see for EC 6.2.1.12 Penicillium chrysogenum ?
-
 ?
6.2.1.30 additional information the enzyme displays broad substrate spectrum and shows higher activities to medium and long-chain fatty acids. The enzyme is also active with cinnamic acid and coumaric acid, see for EC 6.2.1.12 Penicillium chrysogenum WIS 54-1255 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.30 ? x * 61635, sequence calculation Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
6.2.1.30 PAA-CoA ligase
-
 Penicillium chrysogenum
6.2.1.30 PhlC
-
 Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.30 30
-
 assay at Penicillium chrysogenum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.30 0.026
-
 palmitate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.062
-
 stearate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.063
-
 Phenoxyacetate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.073
-
 caprylate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.076
-
 caproate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.099
-
 phenylacetate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.102
-
 caprate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.30 7.5
-
 assay at Penicillium chrysogenum

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.30 ATP
-
 Penicillium chrysogenum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.2.1.30 Penicillium chrysogenum sequence calculation
-
 7.66

Expression

EC Number Organism Comment Expression
6.2.1.30 Penicillium chrysogenum the enzyme is induced by phenylacetate up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.30 0.074
-
 stearate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.124
-
 phenylacetate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.247
-
 Phenoxyacetate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 0.283
-
 palmitate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 1.455
-
 caprate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 1.575
-
 caprylate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum
6.2.1.30 2.917
-
 caproate recombinant enzyme, pH 7.5, 30°C Penicillium chrysogenum