BRENDA - Enzyme Database

Direct and selective small-molecule inhibition of photosynthetic PEP carboxylase: New approach to combat C4 weeds in arable crops

Paulus, J.K.; Foerster, K.; Groth, G.; FEBS Lett. 588, 2101-2106 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.1.31
expressed in Escherichia coli strains BL21(DE) and BL21-Gold(DE)
Flaveria pringlei
4.1.1.31
expressed in Escherichia coli strains BL21(DE) and BL21-Gold(DE)
Flaveria trinervia
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.31
(+)-catechin
-
Flaveria pringlei
4.1.1.31
(+)-catechin
-
Flaveria trinervia
4.1.1.31
2-(4-methoxyphenyl)-3-phenyl-quinoxaline
-
Flaveria trinervia
4.1.1.31
AG 1433
i.e. 2-(3,4-dihydroxyphenyl)-6,7-dimethylquinoxaline
Flaveria pringlei
4.1.1.31
AG 1433
i.e. 2-(3,4-dihydroxyphenyl)-6,7-dimethylquinoxaline
Flaveria trinervia
4.1.1.31
epigallocatechin gallate
-
Flaveria pringlei
4.1.1.31
epigallocatechin gallate
-
Flaveria trinervia
4.1.1.31
additional information
not inhibited by 2-(4-methoxyphenyl)-3-phenyl-quinoxaline
Flaveria pringlei
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.31
phosphate + oxaloacetate
Flaveria pringlei
-
phosphoenolpyruvate + HCO3-
-
-
?
4.1.1.31
phosphate + oxaloacetate
Flaveria trinervia
-
phosphoenolpyruvate + HCO3-
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.31
Flaveria pringlei
Q01647
-
-
4.1.1.31
Flaveria trinervia
P30694
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.31
His-Trap FF or HP column chromatography
Flaveria pringlei
4.1.1.31
His-Trap FF or HP column chromatography
Flaveria trinervia
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.31
phosphate + oxaloacetate
-
727533
Flaveria pringlei
phosphoenolpyruvate + HCO3-
-
-
-
?
4.1.1.31
phosphate + oxaloacetate
-
727533
Flaveria trinervia
phosphoenolpyruvate + HCO3-
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.31
acetyl-CoA
-
Flaveria pringlei
4.1.1.31
acetyl-CoA
-
Flaveria trinervia
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.1.1.31
0.039
-
at pH 8.0 and 25C
Flaveria trinervia
AG 1433
4.1.1.31
0.065
-
at pH 8.0 and 25C
Flaveria trinervia
2-(4-methoxyphenyl)-3-phenyl-quinoxaline
4.1.1.31
0.103
-
at pH 8.0 and 25C
Flaveria trinervia
(+)-catechin
4.1.1.31
0.307
-
at pH 8.0 and 25C
Flaveria pringlei
(+)-catechin
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.31
expressed in Escherichia coli strains BL21(DE) and BL21-Gold(DE)
Flaveria pringlei
4.1.1.31
expressed in Escherichia coli strains BL21(DE) and BL21-Gold(DE)
Flaveria trinervia
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.31
acetyl-CoA
-
Flaveria pringlei
4.1.1.31
acetyl-CoA
-
Flaveria trinervia
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.1.1.31
0.039
-
at pH 8.0 and 25C
Flaveria trinervia
AG 1433
4.1.1.31
0.065
-
at pH 8.0 and 25C
Flaveria trinervia
2-(4-methoxyphenyl)-3-phenyl-quinoxaline
4.1.1.31
0.103
-
at pH 8.0 and 25C
Flaveria trinervia
(+)-catechin
4.1.1.31
0.307
-
at pH 8.0 and 25C
Flaveria pringlei
(+)-catechin
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.31
(+)-catechin
-
Flaveria pringlei
4.1.1.31
(+)-catechin
-
Flaveria trinervia
4.1.1.31
2-(4-methoxyphenyl)-3-phenyl-quinoxaline
-
Flaveria trinervia
4.1.1.31
AG 1433
i.e. 2-(3,4-dihydroxyphenyl)-6,7-dimethylquinoxaline
Flaveria pringlei
4.1.1.31
AG 1433
i.e. 2-(3,4-dihydroxyphenyl)-6,7-dimethylquinoxaline
Flaveria trinervia
4.1.1.31
epigallocatechin gallate
-
Flaveria pringlei
4.1.1.31
epigallocatechin gallate
-
Flaveria trinervia
4.1.1.31
additional information
not inhibited by 2-(4-methoxyphenyl)-3-phenyl-quinoxaline
Flaveria pringlei
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.31
phosphate + oxaloacetate
Flaveria pringlei
-
phosphoenolpyruvate + HCO3-
-
-
?
4.1.1.31
phosphate + oxaloacetate
Flaveria trinervia
-
phosphoenolpyruvate + HCO3-
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.31
His-Trap FF or HP column chromatography
Flaveria pringlei
4.1.1.31
His-Trap FF or HP column chromatography
Flaveria trinervia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.31
phosphate + oxaloacetate
-
727533
Flaveria pringlei
phosphoenolpyruvate + HCO3-
-
-
-
?
4.1.1.31
phosphate + oxaloacetate
-
727533
Flaveria trinervia
phosphoenolpyruvate + HCO3-
-
-
-
?