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Literature summary extracted from

  • McCarver, A.C.; Lessner, D.J.
    Molecular characterization of the thioredoxin system from Methanosarcina acetivorans (2014), FEBS J., 281, 4598-7611.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 expression in Escherichia coli Methanosarcina acetivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.0063
-
 NADPH cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication Methanosarcina acetivorans
1.8.1.9 0.086
-
 thioredoxin disulfide Methanosarcina acetivorans thioredoxin 7, cosubstrate: NADPH, pH and temperature not specified in the publication Methanosarcina acetivorans
1.8.1.9 0.736
-
 NADH cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.9 thioredoxin disulfide + NADPH + H+ Methanosarcina acetivorans
-
 thioredoxin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Methanosarcina acetivorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9
-
 Methanosarcina acetivorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.9 0.3
-
 activity with 5,5'-dithiobis(2-nitrobenzoic acid) and NADPH, pH and temperature not specified in the publication Methanosarcina acetivorans
1.8.1.9 0.5
-
 activity with 5,5'-dithiobis(2-nitrobenzoic acid) and NADH, pH and temperature not specified in the publication Methanosarcina acetivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
-
 Methanosarcina acetivorans 2-nitro-5-thiobenzoate + NAD+
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Methanosarcina acetivorans 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH + H+
-
 Methanosarcina acetivorans thioredoxin + NADP+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH + H+ the enzyme is specific for Methanosarcina acetivorans thioredoxin 7, no activity with Methanosarcina acetivorans thioredoxin 2 or Methanosarcina acetivorans thioredoxin 6 Methanosarcina acetivorans thioredoxin + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 thioredoxin reductase
-
 Methanosarcina acetivorans
1.8.1.9 TrxR
-
 Methanosarcina acetivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 0.65
-
 NADPH cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication Methanosarcina acetivorans
1.8.1.9 0.817
-
 NADH cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication Methanosarcina acetivorans
1.8.1.9 1.175
-
 thioredoxin disulfide Methanosarcina acetivorans thioredoxin 7, cosubstrate: NADPH, pH and temperature not specified in the publication Methanosarcina acetivorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD flavoprotein Methanosarcina acetivorans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.1.9 1.1
-
 NADH cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication Methanosarcina acetivorans
1.8.1.9 13.7
-
 thioredoxin disulfide Methanosarcina acetivorans thioredoxin 7, cosubstrate: NADPH, pH and temperature not specified in the publication Methanosarcina acetivorans
1.8.1.9 103.3
-
 NADPH cosubstrate: 5,5'-dithiobis(2-nitrobenzoic acid), pH and temperature not specified in the publication Methanosarcina acetivorans