Literature summary extracted from
Uehara, R.; Ueda, Y.; You, D.J.; Koga, Y.; Kanaya, S.
Accelerated maturation of Tk-subtilisin by a Leu->Pro mutation at the C-terminus of the propeptide, which reduces the binding of the propeptide to Tk-subtilisin (2013), FEBS J., 280, 994-1006.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.4.21.B57 |
the crystal structure of the complex between L69P-propeptide and S324A-subtilisin (i.e. a protease activity-defective mutant) reveals that the C-terminal region of L69P-propeptide does not well fit into the substrate binding pockets of Tk-subtilisin (S1-S4 subsites) as a result of a conformational change caused by the mutation |
Thermococcus kodakarensis |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.4.21.B57 |
additional information |
the Leu69Pro mutation in the propeptide accelerates the maturation of Pro-Tk-subtilisin by reducing the binding ability of Tk-propeptide to Tk-subtilisin |
Thermococcus kodakarensis |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.21.B57 |
Thermococcus kodakarensis |
P58502 |
sequence including singnal peptide (amino acid 1-24) and propeptide (amino acid 25-106) |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
3.4.21.B57 |
proteolytic modification |
Tk-subtilisin, a subtilisin homologue (Gly70-Gly398) from Thermococcus kodakarensis, is matured from its precursor, Pro-Tk-subtilisin (Tk-subtilisin in a pro form (Gly1-Gly398)), by autoprocessing and degradation of propeptide (Tk-propeptide, a propeptide of Tk-subtilisin (Gly1-Leu69)). The scissile peptide bond between Leu69 and Gly70 of Pro-Tk-subtilisin is first self-cleaved to produce an inactive Tk-propeptide:Tk-subtilisin complex, in which the C-terminal region of Tk-propeptide binds to the active-site cleft of Tk-subtilisin. Tk-propeptide is then dissociated from Tk-subtilisin and degraded by Tk-subtilisin to release active Tk-subtilisin |
Thermococcus kodakarensis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.21.B57 |
Tk-subtilisin |
- |
Thermococcus kodakarensis |