EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.302 | expression in Escherichia coli | Aquifex aeolicus |
1.1.1.302 | expression in Escherichia coli | [Candida] glabrata |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.302 | 25300 | - |
2 * 25300, SDS-PAGE | Aquifex aeolicus |
1.1.1.302 | 28500 | - |
2 * 28500, SDS-PAGE | [Candida] glabrata |
1.1.1.302 | 50600 | - |
sedimentation equilibrium analysis | Aquifex aeolicus |
1.1.1.302 | 57800 | - |
sedimentation equilibrium analysis | [Candida] glabrata |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | Methanocaldococcus jannaschii | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | Aquifex aeolicus | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | [Candida] glabrata | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | Methanocaldococcus jannaschii DSM 2661 | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | [Candida] glabrata ATCC 2001 | biosynthesis of riboflavin | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.302 | Aquifex aeolicus | O66747 | - |
- |
1.1.1.302 | Methanocaldococcus jannaschii | Q58085 | - |
- |
1.1.1.302 | Methanocaldococcus jannaschii DSM 2661 | Q58085 | - |
- |
1.1.1.302 | [Candida] glabrata | Q6FU96 | - |
- |
1.1.1.302 | [Candida] glabrata ATCC 2001 | Q6FU96 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.302 | - |
Aquifex aeolicus |
1.1.1.302 | - |
[Candida] glabrata |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | Methanocaldococcus jannaschii | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | Aquifex aeolicus | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | [Candida] glabrata | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation | Aquifex aeolicus | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate | Methanocaldococcus jannaschii | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate | [Candida] glabrata | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | Methanocaldococcus jannaschii DSM 2661 | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate | Methanocaldococcus jannaschii DSM 2661 | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | biosynthesis of riboflavin | [Candida] glabrata ATCC 2001 | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? | |
1.1.1.302 | 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ | the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate | [Candida] glabrata ATCC 2001 | 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.302 | homodimer | 2 * 28500, SDS-PAGE | [Candida] glabrata |
1.1.1.302 | homodimer | 2 * 25300, SDS-PAGE | Aquifex aeolicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.302 | 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase | - |
Methanocaldococcus jannaschii |
1.1.1.302 | 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase | - |
Aquifex aeolicus |
1.1.1.302 | 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase | - |
[Candida] glabrata |
1.1.1.302 | arfC | - |
Methanocaldococcus jannaschii |
1.1.1.302 | MJ0671 | locus name | Methanocaldococcus jannaschii |
1.1.1.302 | ribD2 | - |
Aquifex aeolicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.302 | 37 | - |
assay at | Methanocaldococcus jannaschii |
1.1.1.302 | 37 | - |
assay at | Aquifex aeolicus |
1.1.1.302 | 37 | - |
assay at | [Candida] glabrata |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.302 | 8.2 | - |
assay at | Methanocaldococcus jannaschii |
1.1.1.302 | 8.2 | - |
assay at | [Candida] glabrata |
1.1.1.302 | 8.2 | - |
asay at | Aquifex aeolicus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.302 | physiological function | biosynthesis of riboflavin | Methanocaldococcus jannaschii |
1.1.1.302 | physiological function | biosynthesis of riboflavin | Aquifex aeolicus |
1.1.1.302 | physiological function | biosynthesis of riboflavin | [Candida] glabrata |