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Literature summary extracted from

  • Frick, E.; Spatzal, T.; Gerhardt, S.; Krämer, A.; Einsle, O.; Hüttel, W.
    Structural and functional characterization of 4-hydroxyphenylpyruvate dioxygenase from the thermoacidophilic archaeon Picrophilus torridus (2014), Extremophiles, 18, 641-651.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.27
-
 Picrophilus torridus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.27 crystals are grown by sitting-drop vapor diffusion method at 20°C Picrophilus torridus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.27 2-mercaptoethanol 10 mM, 65% inhibition Picrophilus torridus
1.13.11.27 4-hydroxyphenylpyruvate substrate inhibition Picrophilus torridus
1.13.11.27 Al3+ 10 mM, 7% inhibition Picrophilus torridus
1.13.11.27 Ca2+ 10 mM, 16% inhibition Picrophilus torridus
1.13.11.27 Co2+ 10 mM, 32% inhibition Picrophilus torridus
1.13.11.27 Cu2+ 10 mM, complete inhibition Picrophilus torridus
1.13.11.27 DTT 10 mM, 61% inhibition Picrophilus torridus
1.13.11.27 EDTA 10 mM, 76% inhibition Picrophilus torridus
1.13.11.27 Fe2+ 10 mM, 82% inhibition Picrophilus torridus
1.13.11.27 K+ 10 mM, 6% inhibition Picrophilus torridus
1.13.11.27 Mg2+ 10 mM, 18% inhibition Picrophilus torridus
1.13.11.27 Mn2+ 10 mM, 97% inhibition Picrophilus torridus
1.13.11.27 Na+ 10 mM, 14% inhibition Picrophilus torridus
1.13.11.27 Ni2+ 10 mM, 28% inhibition Picrophilus torridus
1.13.11.27 SDS 2% (w/v), complete inhibition Picrophilus torridus
1.13.11.27 Urea 1 M, 3% inhibition. 3 M, 42% inhibition. 5 M, 56% inhibition Picrophilus torridus
1.13.11.27 Zn2+ 10 mM, 65% inhibition Picrophilus torridus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.27 0.142
-
 4-hydroxyphenylpyruvate pH 7.0, 50°C Picrophilus torridus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2 Picrophilus torridus second step of oxidative tyrosine catabolism homogentisate + CO2
-
 ?
1.13.11.27 4-hydroxyphenylpyruvate + O2 Picrophilus torridus DSM 9790 second step of oxidative tyrosine catabolism homogentisate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Picrophilus torridus Q6KZ98
-
-
1.13.11.27 Picrophilus torridus DSM 9790 Q6KZ98
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.27
-
 Picrophilus torridus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Picrophilus torridus homogentisate + CO2
-
 ?
1.13.11.27 4-hydroxyphenylpyruvate + O2 second step of oxidative tyrosine catabolism Picrophilus torridus homogentisate + CO2
-
 ?
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Picrophilus torridus DSM 9790 homogentisate + CO2
-
 ?
1.13.11.27 4-hydroxyphenylpyruvate + O2 second step of oxidative tyrosine catabolism Picrophilus torridus DSM 9790 homogentisate + CO2
-
 ?
1.13.11.27 additional information no activity is observed for 3-hydroxyphenylpyruvate, 3,4-dihydroxyphenylpyruvate, 4-hydroxyphenylglyoxylate, phenylpyruvate, 2-oxoglutarate, glyoxylate, pyruvate, oxaloacetate or oxobutyrate Picrophilus torridus ?
-
 ?
1.13.11.27 additional information no activity is observed for 3-hydroxyphenylpyruvate, 3,4-dihydroxyphenylpyruvate, 4-hydroxyphenylglyoxylate, phenylpyruvate, 2-oxoglutarate, glyoxylate, pyruvate, oxaloacetate or oxobutyrate Picrophilus torridus DSM 9790 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.27 PTO1369 locus name Picrophilus torridus
1.13.11.27 Pt_Hpd
-
 Picrophilus torridus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.27 50
-
 maximal activity when assayed at pH 4.0 or 7.0 Picrophilus torridus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.27 30 70 30°C: about 65% of maximal activity, 70°C: about 30% of maximal activity, assayed at pH 7.0 Picrophilus torridus
1.13.11.27 40 60 40°C: about 55% of maximal activity, 60°C: about 40% of maximal activity, assayed at pH 4.0 Picrophilus torridus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.27 40
-
 pH 4.0, half-life: 1.5 h Picrophilus torridus
1.13.11.27 77
-
 strong conformational changes are observed at 77°C, indicating melting of the protein Picrophilus torridus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.27 9.9
-
 4-hydroxyphenylpyruvate pH 7.0, 50°C Picrophilus torridus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.27 4
-
 two pH optima are observed at 50°C: pH 4.0 (close to native conditions, narrow maximum) and pH 7.0 (broad maximum) Picrophilus torridus
1.13.11.27 7
-
 two pH optima are observed at 50°C: pH 4.0 (close to native conditions, narrow maximum) and pH 7.0 (broad maximum) Picrophilus torridus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.27 1.9
-
 4-hydroxyphenylpyruvate pH 7.0, 50°C Picrophilus torridus

General Information

EC Number General Information Comment Organism
1.13.11.27 metabolism second step of oxidative tyrosine catabolism Picrophilus torridus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.27 69.7
-
 4-hydroxyphenylpyruvate pH 7.0, 50°C Picrophilus torridus