EC Number | General Stability | Organism |
---|---|---|
2.1.1.86 | during purification, the enzyme is stabilized with 0.01 mM hydroxocobalamin | Methanosarcina mazei |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.1.1.86 | membrane | membrane associated enzyme | Methanosarcina mazei | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.86 | Na+ | maximum activity at 1 mM Na+ | Methanosarcina mazei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.86 | 9000 | - |
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
2.1.1.86 | 12000 | - |
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
2.1.1.86 | 13000 | - |
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
2.1.1.86 | 20000 | - |
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
2.1.1.86 | 28000 | - |
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
2.1.1.86 | 34000 | - |
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
2.1.1.86 | 380000 | - |
non-denaturing gradient PAGE | Methanosarcina mazei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.86 | 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate | Methanosarcina mazei | 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump | 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate | - |
? | |
2.1.1.86 | 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate | Methanosarcina mazei DSM 3647 | 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump | 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.86 | Methanosarcina mazei | P80655 and P80653 and P80651 and P80654 and P80656 and P80650 | P80655: subunit B, P80653: subunit D, P80651: subunit E, P80654: subunit F, P80656: subunit G, P80650: subunit H | - |
2.1.1.86 | Methanosarcina mazei DSM 3647 | P80655 and P80653 and P80651 and P80654 and P80656 and P80650 | P80655: subunit B, P80653: subunit D, P80651: subunit E, P80654: subunit F, P80656: subunit G, P80650: subunit H | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.86 | - |
Methanosarcina mazei |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.86 | culture condition:acetate-grown cell | - |
Methanosarcina mazei | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.86 | 10.4 | - |
37°C, pH 6.9 | Methanosarcina mazei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.86 | 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate | 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump | Methanosarcina mazei | 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate | - |
? | |
2.1.1.86 | 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate | 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump. 1.7 mol Na+ per mol of methyl groups transferred are translocated | Methanosarcina mazei | 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate | - |
? | |
2.1.1.86 | 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate | 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump | Methanosarcina mazei DSM 3647 | 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate | - |
? | |
2.1.1.86 | 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate | 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump. 1.7 mol Na+ per mol of methyl groups transferred are translocated | Methanosarcina mazei DSM 3647 | 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate | - |
? | |
2.1.1.86 | methylcobalamin + 2-mercaptoethanesulfonate | at 13.6% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin | Methanosarcina mazei | ? | - |
? | |
2.1.1.86 | methylcobalamin + 2-mercaptoethanesulfonate | at 13.6% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin | Methanosarcina mazei DSM 3647 | ? | - |
? | |
2.1.1.86 | N5-methyltetrahydrofolate + 2-mercaptoethanesulfonate | at 31.8% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin | Methanosarcina mazei | ? | - |
? | |
2.1.1.86 | N5-methyltetrahydrofolate + 2-mercaptoethanesulfonate | at 31.8% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin | Methanosarcina mazei DSM 3647 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.86 | ? | x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE | Methanosarcina mazei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.86 | H,MPT:coenzyme M methyltransferase | - |
Methanosarcina mazei |
2.1.1.86 | N5-methyltetrahydromethanopterin: coenzyme M methyltransferase | - |
Methanosarcina mazei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.86 | 37 | - |
assay at | Methanosarcina mazei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.86 | 7 | 7.5 | - |
Methanosarcina mazei |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.86 | corrinoid | - |
Methanosarcina mazei |