Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lienard, T.; Becher, B.; Marschall, M.; Bowien, S.; Gottschalk, G.
    Sodium ion translocation by N5-methyltetrahydromethanopterin: coenzyme M methyltransferase from Methanosarcina mazei Gö1 reconstituted in ether lipid liposomes (1996), Eur. J. Biochem., 239, 857-864.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.86 during purification, the enzyme is stabilized with 0.01 mM hydroxocobalamin Methanosarcina mazei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.86 membrane membrane associated enzyme Methanosarcina mazei 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.86 Na+ maximum activity at 1 mM Na+ Methanosarcina mazei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.86 9000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE Methanosarcina mazei
2.1.1.86 12000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE Methanosarcina mazei
2.1.1.86 13000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE Methanosarcina mazei
2.1.1.86 20000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE Methanosarcina mazei
2.1.1.86 28000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE Methanosarcina mazei
2.1.1.86 34000
-
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE Methanosarcina mazei
2.1.1.86 380000
-
non-denaturing gradient PAGE Methanosarcina mazei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate Methanosarcina mazei 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
?
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate Methanosarcina mazei DSM 3647 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.86 Methanosarcina mazei P80655 and P80653 and P80651 and P80654 and P80656 and P80650 P80655: subunit B, P80653: subunit D, P80651: subunit E, P80654: subunit F, P80656: subunit G, P80650: subunit H
-
2.1.1.86 Methanosarcina mazei DSM 3647 P80655 and P80653 and P80651 and P80654 and P80656 and P80650 P80655: subunit B, P80653: subunit D, P80651: subunit E, P80654: subunit F, P80656: subunit G, P80650: subunit H
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.86
-
Methanosarcina mazei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.86 culture condition:acetate-grown cell
-
Methanosarcina mazei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.86 10.4
-
37°C, pH 6.9 Methanosarcina mazei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump Methanosarcina mazei 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
?
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump. 1.7 mol Na+ per mol of methyl groups transferred are translocated Methanosarcina mazei 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
?
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump Methanosarcina mazei DSM 3647 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
?
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate 2-mercaptoethanesulfonate i.e. coenzyme M. The enzyme uses the methylation of coenzyme M (HS-CoM) by methyl-tetrahydromethanopterin to drive an energy-conserving sodium ion pump. 1.7 mol Na+ per mol of methyl groups transferred are translocated Methanosarcina mazei DSM 3647 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
-
?
2.1.1.86 methylcobalamin + 2-mercaptoethanesulfonate at 13.6% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin Methanosarcina mazei ?
-
?
2.1.1.86 methylcobalamin + 2-mercaptoethanesulfonate at 13.6% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin Methanosarcina mazei DSM 3647 ?
-
?
2.1.1.86 N5-methyltetrahydrofolate + 2-mercaptoethanesulfonate at 31.8% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin Methanosarcina mazei ?
-
?
2.1.1.86 N5-methyltetrahydrofolate + 2-mercaptoethanesulfonate at 31.8% of the activity compared to 5-methyl-5,6,7,8-tetrahydromethanopterin Methanosarcina mazei DSM 3647 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.86 ? x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE Methanosarcina mazei

Synonyms

EC Number Synonyms Comment Organism
2.1.1.86 H,MPT:coenzyme M methyltransferase
-
Methanosarcina mazei
2.1.1.86 N5-methyltetrahydromethanopterin: coenzyme M methyltransferase
-
Methanosarcina mazei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.86 37
-
assay at Methanosarcina mazei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.86 7 7.5
-
Methanosarcina mazei

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.86 corrinoid
-
Methanosarcina mazei