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Literature summary extracted from

  • Feil, I.K.; Lerch, H.P.; Schomburg, D.
    Deletion variants of L-hydroxyisocaproate dehydrogenase. Probing substrate specificity (1994), Eur. J. Biochem., 223, 857-863.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.337 del I81/delK82 phenylpyruvate is the only substrate which is converted at a significant catalytic rate by the mutant enzyme. In the publication this mutant is referred to as Ile100ADELTA/Lys100BDELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
1.1.1.337 del I81/delK82/delN87/delP88 specific modifications in catalytic rates and substrate recognition Weissella confusa
1.1.1.337 del L 83 deletion mutant shows an altered substrate specificity. In the publication this mutant is referred to as Leu101DELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
1.1.1.337 delI81 drastic reductions in the catalytic activity for all tested substrates. In the publication this mutant is referred to as Ile100ADELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
1.1.1.337 delK82 drastic reductions in the catalytic activity for all tested substrates. In the publication this mutant is referred to as Lys100BDELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
1.1.1.337 delN87 deletion mutant shows an altered substrate specificity. In the publication this mutant is referred to as Asn105ADELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
1.1.1.337 delN87/delP88 for the deletion mutant enzyme 2-oxo carboxylic acids branched at C4 are better substrates than 2-oxocaproate, the substrate with the best kcat,/KM ratio known for the wild-type enzyme.The mutation results in a 5.2fold increased catalytic efficiency towards 4-methyl-2-oxopentanoate compared to the wild-type enzyme. In the publication this mutant is referred to as Asn105ADELTA/Pro105BDELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
1.1.1.337 delP88 deletion mutant shows an altered substrate specificity. In the publication this mutant is referred to as Pro105BDELTA, according to the numbering system of the dogfish L-lactate dehydrogenase coenzyme loop region Weissella confusa
1.1.1.337 additional information the coenzyme loop, a functional element which is essential for catalysis and substrate specificity, is modified in order to identify the residues involved in the catalytic reaction and substrate specificity Weissella confusa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.337 0.026
-
 phenylpyruvate pH 7.0, 30°C, wild-type enzyme Weissella confusa
1.1.1.337 0.067
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, wild-type enzyme Weissella confusa
1.1.1.337 0.071
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.1.1.337 0.1 1 2-oxocaproate pH 7.0, 30°C, wild-type enzyme Weissella confusa
1.1.1.337 0.1 2 phenylpyruvate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.1.1.337 0.17
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.1.1.337 0.33
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
1.1.1.337 0.41
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1.1.1.337 0.62
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
1.1.1.337 0.9 1 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1.1.1.337 1 4 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
1.1.1.337 2
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1.1.1.337 2 5 pyruvate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1.1.1.337 2.8
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
1.1.1.337 2.8
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del L83 Weissella confusa
1.1.1.337 2.8
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
1.1.1.337 3.3
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
1.1.1.337 3.5
-
 pyruvate pH 7.0, 30°C, wild-type enzyme Weissella confusa
1.1.1.337 5
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1.1.1.337 5.8
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.1.1.337 6.4
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del L83 Weissella confusa
1.1.1.337 9.3
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.1.1.337 11
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del L83 Weissella confusa
1.1.1.337 18
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.1.1.337 18
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.1.1.337 19
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
1.1.1.337 19
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1.1.1.337 27
-
 pyruvate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
1.1.1.337 30
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
1.1.1.337 43
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
1.1.1.337 50
-
 pyruvate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.1.1.337 62
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
1.1.1.337 110
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1.1.1.337 140
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
1.1.1.337 200
-
 pyruvate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.337 Weissella confusa P14295 i.e. Lactobacillus confusus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.337
-
 Weissella confusa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.337 2-oxocaproate + NADH + H+
-
 Weissella confusa 2-hydroxycaproate + NAD+
-
 r
1.1.1.337 4-methyl-2-oxopentanoate + NADH + H+
-
 Weissella confusa (S)-2-hydroxy-4-methylpentanoate + NAD+
-
 r
1.1.1.337 phenylpyruvate + NADH + H+
-
 Weissella confusa 3-phenyllactate + NAD+
-
 r
1.1.1.337 pyruvate + NADH + H+
-
 Weissella confusa L-lactate + NAD+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.337 L-2-hydroxyisocaproate dehydrogenase
-
 Weissella confusa
1.1.1.337 L-HicDH
-
 Weissella confusa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.337 30
-
 assay at Weissella confusa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.337 0.004
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
1.1.1.337 0.03
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
1.1.1.337 0.23
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.1.1.337 0.63
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.1.1.337 0.76
-
 pyruvate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1.1.1.337 1.7
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.1.1.337 8
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.1.1.337 13
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
1.1.1.337 15
-
 pyruvate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1.1.1.337 17
-
 pyruvate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
1.1.1.337 20
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
1.1.1.337 25
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
1.1.1.337 39
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
1.1.1.337 40
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1.1.1.337 46
-
 pyruvate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.1.1.337 90
-
 pyruvate pH 7.0, 30°C, wild-type enzyme Weissella confusa
1.1.1.337 280
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1.1.1.337 490
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.1.1.337 620
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.1.1.337 750
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.1.1.337 920
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1.1.1.337 1000
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del L 83 Weissella confusa
1.1.1.337 1200
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del L 83 Weissella confusa
1.1.1.337 2400
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, wild-type enzyme Weissella confusa
1.1.1.337 2600
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
1.1.1.337 4200
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del L 83 Weissella confusa
1.1.1.337 4400
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1.1.1.337 7300
-
 phenylpyruvate pH 7.0, 30°C, wild-type enzyme Weissella confusa
1.1.1.337 12000
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
1.1.1.337 15000
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1.1.1.337 19000
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1.1.1.337 21000
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
1.1.1.337 89000
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
1.1.1.337 360000
-
 2-oxocaproate pH 7.0, 30°C, wild-type enzyme Weissella confusa
1.1.1.337 520000
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.337 7
-
 assay at Weissella confusa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.337 0.00013
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
1.1.1.337 0.00021
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
1.1.1.337 0.0038
-
 pyruvate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1.1.1.337 0.025
-
 pyruvate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.1.1.337 0.035
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.1.1.337 0.092
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.1.1.337 0.32
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
1.1.1.337 0.6
-
 pyruvate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1.1.1.337 0.63
-
 pyruvate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
1.1.1.337 0.68
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
1.1.1.337 0.91
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
1.1.1.337 0.92
-
 pyruvate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.1.1.337 1.4
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81 Weissella confusa
1.1.1.337 1.8
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
1.1.1.337 8
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1.1.1.337 8.4
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1.1.1.337 15
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delK82 Weissella confusa
1.1.1.337 26
-
 pyruvate pH 7.0, 30°C, wild-type enzyme Weissella confusa
1.1.1.337 160
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme del L 83 Weissella confusa
1.1.1.337 380
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme del L 83 Weissella confusa
1.1.1.337 790
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82 Weissella confusa
1.1.1.337 2200
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1.1.1.337 2900
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.1.1.337 4300
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del I81/delK82/delN87/delP88 Weissella confusa
1.1.1.337 4300
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme del L 83 Weissella confusa
1.1.1.337 6300
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.1.1.337 8700
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delP88 Weissella confusa
1.1.1.337 16000
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1.1.1.337 34000
-
 2-oxocaproate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
1.1.1.337 36000
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, wild-type enzyme Weissella confusa
1.1.1.337 46000
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delN87 Weissella confusa
1.1.1.337 190000
-
 4-methyl-2-oxopentanoate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
1.1.1.337 270000
-
 phenylpyruvate pH 7.0, 30°C, mutant enzyme delN87/delP88 Weissella confusa
1.1.1.337 280000
-
 phenylpyruvate pH 7.0, 30°C, wild-type enzyme Weissella confusa
1.1.1.337 3300000
-
 2-oxocaproate pH 7.0, 30°C, wild-type enzyme Weissella confusa