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Literature summary extracted from

  • Vyazmensky, M.; Barak, Z.; Chipman, D.M., Eichler, J.
    Characterization of acetohydroxy acid synthase activity in the archaeon Haloferax volcanii (2000), Comp. Biochem. Physiol. B Biochem. Mol. Biol., 125, 205-210.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 EDTA dialysis against EDTA leads to an irreversible loss of activity Haloferax volcanii
2.2.1.6 L-isoleucine weakly Haloferax volcanii
2.2.1.6 L-valine inhibits to a maximal activity of approximately 50% at concentrations higher than 0.5 mM Haloferax volcanii
2.2.1.6 Mn2+ activates, high concentrations inhibit Haloferax volcanii
2.2.1.6 additional information leucine had negligible effect on the activity Haloferax volcanii
2.2.1.6 sulfometuron methyl
-
 Haloferax volcanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 25.5
-
 pyruvate pH 7.5, 37°C, in the presence of 3.5 M KCl Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 KCl maximal activity required KCl concentrations of greater than 3.5 M Haloferax volcanii
2.2.1.6 Mg2+ Mg2 is probably the natural cofactor of the enzyme. Halofgerax volcanii is thought to contain a high intracellular Mg2+ concentration, as its native habitat, the Dead Sea, contains 1.4 M MgCl2. The endogenous metal is apparently tightly-enough bound so that added Mg2+ does not stimulate the activity Haloferax volcanii
2.2.1.6 Mn2+ activates, high concentrations inhibit Haloferax volcanii
2.2.1.6 NaCl while raising the NaCl concentration from 1.5 to 3.5 M leads to an increase in enzyme activity, this increase is 3fold lower than that detected in the presence of optimal KCl levels Haloferax volcanii

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Haloferax volcanii
-
-
-
2.2.1.6 Haloferax volcanii DSM 3757
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.6 0.0043
-
 substrate: pyruvate, pH 7.5, 37°C, in the presence of 3.5 M KCl Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Haloferax volcanii 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate
-
 Haloferax volcanii DSM 3757 2-acetolactate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 acetohydroxy acid synthase
-
 Haloferax volcanii
2.2.1.6 AHAS
-
 Haloferax volcanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.6 35 40
-
 Haloferax volcanii
2.2.1.6 37
-
 assay at Haloferax volcanii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.2.1.6 25 45 activity can be detected between 25 and 45°C, with the 35–40°C range being optimal. A rapid loss in catalytic activity is observed above 45°C Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 7.5
-
 assay at Haloferax volcanii

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 thiamine diphosphate required cofactor, apparent Km: 0.0088 mM Haloferax volcanii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.2.1.6 0.005
-
 sulfometuron methyl pH 7.5, 37°C, in the presence of 3.5 M KCl Haloferax volcanii