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Literature summary extracted from

  • Moor, N.; Klipcan, L.; Safro, M.G.
    Bacterial and eukaryotic phenylalanyl-tRNA synthetases catalyze misaminoacylation of tRNAPhe with 3,4-dihydroxy-L-phenylalanine (2011), Chem. Biol., 18, 1221-1229.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.20 in complex with 3,4-dihydroxy-L-phenylalanine Thermus thermophilus
6.1.1.20 in complex with 3,4-dihydroxy-L-phenylalanine Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.20 DL-m-tyrosine
-
 Homo sapiens
6.1.1.20 L-tyrosine
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.20 0.0017
-
 L-phenylalanine in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Thermus thermophilus
6.1.1.20 0.0026
-
 L-phenylalanine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.0032
-
 L-phenylalanine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.012
-
 DL-m-tyrosine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.13
-
 DL-m-tyrosine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.38
-
 3,4-dihydroxy-L-phenylalanine in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Thermus thermophilus
6.1.1.20 0.6
-
 3,4-dihydroxy-L-phenylalanine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.65
-
 3,4-dihydroxy-L-phenylalanine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 1.9
-
 L-tyrosine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.20 cytoplasm
-
 Homo sapiens 5737
-
6.1.1.20 mitochondrion
-
 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe Thermus thermophilus
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-phenylalanine + tRNAPhe Homo sapiens
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Homo sapiens
-
-
-
6.1.1.20 Homo sapiens O95363
-
-
6.1.1.20 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + 3,4-dihydroxy-L-phenylalanine + tRNAPhe
-
 Thermus thermophilus AMP + diphosphate + 3,4-dihydroxy-L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + 3,4-dihydroxy-L-phenylalanine + tRNAPhe 0.13% activity compared to L-phenylalanine Homo sapiens AMP + diphosphate + 3,4-dihydroxy-L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + 3,4-dihydroxy-L-phenylalanine + tRNAPhe 0.33% activity compared to L-phenylalanine Homo sapiens AMP + diphosphate + 3,4-dihydroxy-L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + DL-m-tyrosine + tRNAPhe 1.9% activity compared to L-phenylalanine Homo sapiens AMP + diphosphate + DL-m-tyrosyl-tRNAPhe
-
 ?
6.1.1.20 ATP + DL-m-tyrosine + tRNAPhe 22% activity compared to L-phenylalanine Homo sapiens AMP + diphosphate + DL-m-tyrosyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Thermus thermophilus AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Homo sapiens AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-phenylalanine + tRNAPhe 100% activity Homo sapiens AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 ATP + L-tyrosine + tRNAPhe 0.089% activity compared to L-phenylalanine Homo sapiens AMP + diphosphate + L-tyrosyl-tRNAPhe
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.20 Phenylalanyl-tRNA synthetase
-
 Thermus thermophilus
6.1.1.20 Phenylalanyl-tRNA synthetase
-
 Homo sapiens
6.1.1.20 PheRS
-
 Thermus thermophilus
6.1.1.20 PheRS
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.20 0.033
-
 L-tyrosine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.052
-
 DL-m-tyrosine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.057
-
 3,4-dihydroxy-L-phenylalanine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.075
-
 L-phenylalanine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.16
-
 3,4-dihydroxy-L-phenylalanine in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Thermus thermophilus
6.1.1.20 0.23
-
 3,4-dihydroxy-L-phenylalanine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.65
-
 DL-m-tyrosine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.87
-
 L-phenylalanine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 1.12
-
 L-phenylalanine in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.20 ATP
-
 Thermus thermophilus
6.1.1.20 ATP
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.1.1.20 0.0136
-
 DL-m-tyrosine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.15
-
 DL-m-tyrosine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 3.4
-
 L-tyrosine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.20 0.017
-
 L-tyrosine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.095
-
 3,4-dihydroxy-L-phenylalanine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.37
-
 3,4-dihydroxy-L-phenylalanine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 0.43
-
 3,4-dihydroxy-L-phenylalanine in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Thermus thermophilus
6.1.1.20 4.3
-
 DL-m-tyrosine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 5
-
 DL-m-tyrosine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 28.3
-
 L-phenylalanine mitochondrial enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 270
-
 L-phenylalanine cytoplasmic enzyme, in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Homo sapiens
6.1.1.20 650
-
 L-phenylalanine in 50 mM Tris-HCl, pH 8.0, 30 mM MgCl2, 20 mM KCl, 5 mM dithiothreitol, at 30°C Thermus thermophilus